The importance of particle type selection and temperature control for on-chip free-flow magnetophoresis

Tarn, Mark D.; Peyman, Sally A.; Robert, Damien; Iles, Alexander; Wilhelm, Claire; Pamme, Nicole

doi:10.1016/j.jmmm.2009.08.016

Cited by 47 publications

(34 citation statements)

References 23 publications

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“…It has been reported that the separation efficiency is also affected by temperature, and as the temperature increases, the separation efficiency increases due to a decrease in fluid viscosity. 11 The use of continuous-flow magnetophoresis for bio-separation has been growing in recent years. The biological materials that microfluidic magnetophoresis can target range in size from DNA to biological cells.…”

Section: Introductionmentioning

confidence: 99%

Magnetophoretic-based microfluidic device for DNA isolation

Hale

Darabi

2014

Biomicrofluidics

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This paper presents a continuous flow microfluidic device for the separation of DNA from blood using magnetophoresis for biological applications and analysis. This microfluidic bio-separation device has several benefits, including decreased sample handling, smaller sample and reagent volumes, faster isolation time, and decreased cost to perform DNA isolation. One of the key features of this device is the use of short-range magnetic field gradients, generated by a micro-patterned nickel array on the bottom surface of the separation channel. In addition, the device utilizes an array of oppositely oriented, external permanent magnets to produce strong long-range field gradients at the interfaces between magnets, further increasing the effectiveness of the device. A comprehensive simulation is performed using COMSOL Multiphysics to study the effect of various parameters on the magnetic flux within the separation channel. Additionally, a microfluidic device is designed, fabricated, and tested to isolate DNA from blood. The results show that the device has the capability of separating DNA from a blood sample with a purity of 1.8 or higher, a yield of up to 33 lg of polymerase chain reaction ready DNA per milliliter of blood, and a volumetric throughput of up to 50 ml/h. V C 2014 AIP Publishing LLC.

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Section: Introductionmentioning

confidence: 99%

Magnetophoretic-based microfluidic device for DNA isolation

Hale

Darabi

2014

Biomicrofluidics

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“…Then, an external magnetic force acts on the suspended particles and deflects them to different flow paths in the sheath stream for a continuous sorting. This technique has been demonstrated for the separations of magnetic from diamagnetic (often called nonmagnetic by non-physicists) particles, [17][18][19] magnetic from magnetic particles, [20][21][22] and diamagnetic from diamagnetic particles. [23][24][25] Its main drawback lies in the use of a sheath flow to focus particles, which not only complicates the flow control and device fabrication but also dilutes the separated particles at the cost of chemicals.…”

Section: Introductionmentioning

confidence: 99%

Continuous sheath-free magnetic separation of particles in a U-shaped microchannel

Liang¹,

Xuan²

2012

Biomicrofluidics

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Particle separation is important to many chemical and biomedical applications. Magnetic field-induced particle separation is simple, cheap, and free of fluid heating issues that accompany electric, acoustic, and optical methods. We develop herein a novel microfluidic approach to continuous sheath-free magnetic separation of particles. This approach exploits the negative or positive magnetophoretic deflection to focus and separate particles in the two branches of a U-shaped microchannel, respectively. It is applicable to both magnetic and diamagnetic particle separations, and is demonstrated through the sorting of 5 lm and 15 lm polystyrene particles suspended in a dilute ferrofluid. V C 2012 American Institute of Physics. [http://dx

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“…Park et al [15] fractionated particles by using three parallel fluid flows with different magnetic susceptibilities; here the sample particles were introduced in the central flow and a magnetic force caused them to migrate to the adjacent fluid flow. The magnetophoretic microchip has also been reported; it has a thin inlet channel and a broad separation chamber to apply the magnetic force perpendicular to the flow direction [16]. Twenty-five outlet flow channels were fabricated to fractionate biological particles by a perpendicular magnetic force against the flow direction [17].…”

Section: Introductionmentioning

confidence: 99%

Two-dimensional flow magnetophoresis of microparticles

Kawano

Watarai

2012

Anal Bioanal Chem

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A new two-dimensional micro-flow magnetophoresis device was constructed in a superconducting magnet (10 T) using triangular shaped pole pieces, which could apply a magnetic strength, B(dB/dx), in the range of ca. 0-14,000 T(2) m(-1) across a capillary cell. Polystyrene particles with diameters of 1, 3, and 6 μm were used as test samples in a paramagnetic medium of 1 M MnCl(2) to evaluate the performance of this method. Microparticles migrated across the capillary along the edge of the pole pieces, and then flowed through the gap in the pole piece at a position defined as the migration distance, depending on the magnetic susceptibility and the size of particles as well as the flow rate. The most effective flow rate to exhibit the largest resolution among the particles was theoretically predicted and experimentally confirmed. By this method, the magnetic susceptibilities of individual deoxygenated and non-deoxygenated red blood cells were measured from the relative migration distance.

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The importance of particle type selection and temperature control for on-chip free-flow magnetophoresis

Cited by 47 publications

References 23 publications

Magnetophoretic-based microfluidic device for DNA isolation

Magnetophoretic-based microfluidic device for DNA isolation

Continuous sheath-free magnetic separation of particles in a U-shaped microchannel

Two-dimensional flow magnetophoresis of microparticles

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