Continuous sheath-free magnetic separation of particles in a U-shaped microchannel

Liang, Litao; Xuan, Xiangchun

doi:10.1063/1.4765335

Cited by 43 publications

(41 citation statements)

References 41 publications

(37 reference statements)

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“…Trapping and concentration of the yeast cells [141], mouse fibroblast and algea [111] have been achieved. The diamagnetic separation principle can also be applied for separation by size [102]. The yeast cells have been successfully separated from each other in EMG408 ferrofluid [142] with a permanent magnet.…”

Section: Applicationmentioning

confidence: 99%

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Microfluidic bio-particle manipulation for biotechnology

Çetin

Özer

Solmaz

2014

Biochemical Engineering Journal

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a b s t r a c tMicrofluidics and lab-on-a-chip technology offers unique advantages for the next generation devices for diagnostic therapeutic applications. For chemical, biological and biomedical analysis in microfluidic systems, there are some fundamental operations such as separation, focusing, filtering, concentration, trapping, detection, sorting, counting, washing, lysis of bio-particles, and PCR-like reactions. The combination of these operations led to the complete analysis systems for specific applications. Manipulation of the bio-particles is the key ingredient for these applications. Therefore, microfluidic bio-particle manipulation has attracted a significant attention from the academic community. Considering the size of the bio-particles and the throughput of the practical applications, manipulation of the bio-particles is a challenging problem. Different techniques are available for the manipulation of bio-particles in microfluidic systems. In this review, some of the techniques for the manipulation of bio-particles; namely hydrodynamic based, electrokinetic-based, acoustic-based, magnetic-based and optical-based methods have been discussed. The comparison of different techniques and the recent applications regarding the microfluidic bio-particle manipulation for different biotechnology applications are presented. Finally, challenges and the future research directions for microfluidic bio-particle manipulation are addressed.

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Section: Applicationmentioning

confidence: 99%

“…Due to the ease of process and possibility of embedding the magnets into the device, most widely used material is PDMS [48,64,98,99,65,[100][101][102]. Other materials are also used in this method such as PMMA [66] and silica [76].…”

Section: Materials and Fabricationmentioning

confidence: 99%

Microfluidic bio-particle manipulation for biotechnology

Çetin

Özer

Solmaz

2014

Biochemical Engineering Journal

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“…These forces include hydrodynamic drag force, gravitational force, and magnetic force. There are varying assumptions made by researchers in this field but the ones that are the most common are a Newtonian fluid (for the sample or the buffer solution), 6 spherical magnetic particles, 21 non-rotational, 22 no slip conditions for the walls of the device, 23 and laminar flow. 24,25 The governing equations are Navier-Stokes for fluid flow and Maxwell's equations for magnetic fields.…”

Section: Theoretical Backgroundmentioning

confidence: 99%

Magnetophoretic-based microfluidic device for DNA isolation

Hale

Darabi

2014

Biomicrofluidics

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This paper presents a continuous flow microfluidic device for the separation of DNA from blood using magnetophoresis for biological applications and analysis. This microfluidic bio-separation device has several benefits, including decreased sample handling, smaller sample and reagent volumes, faster isolation time, and decreased cost to perform DNA isolation. One of the key features of this device is the use of short-range magnetic field gradients, generated by a micro-patterned nickel array on the bottom surface of the separation channel. In addition, the device utilizes an array of oppositely oriented, external permanent magnets to produce strong long-range field gradients at the interfaces between magnets, further increasing the effectiveness of the device. A comprehensive simulation is performed using COMSOL Multiphysics to study the effect of various parameters on the magnetic flux within the separation channel. Additionally, a microfluidic device is designed, fabricated, and tested to isolate DNA from blood. The results show that the device has the capability of separating DNA from a blood sample with a purity of 1.8 or higher, a yield of up to 33 lg of polymerase chain reaction ready DNA per milliliter of blood, and a volumetric throughput of up to 50 ml/h. V C 2014 AIP Publishing LLC.

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“…Because ferrofluids usually have a much higher magnetic susceptibility than that of paramagnetic solutions, permanent magnets or electromagnets are sufficient to induce enough magnetophoretic force to manipulate nonmagnetic particles in microfluidics 22 . Many previous studies have reported focusing and separation of microparticles and cells using ferrofluid in microfluidics 5,16,18,[23][24][25] . In those studies, the non-magnetic particle suspension needs to be first confined by a co-flowing sheath flow; and in the downstream an external magnetic field acts on the nonmagnetic particles and deflects them into different paths 16,25 .…”

Section: Introductionmentioning

confidence: 99%

“…In those studies, the non-magnetic particle suspension needs to be first confined by a co-flowing sheath flow; and in the downstream an external magnetic field acts on the nonmagnetic particles and deflects them into different paths 16,25 . It is noted that the utilization of sheath flow not only complicates the flow control and device fabrication, but also dilutes the sorted particles 24 . Alternatively, the employment of two offset magnets 26 or two arrays of permanent magnetsfocusing and continuous separation of particles.…”

Section: Introductionmentioning

confidence: 99%

A novel viscoelastic-based ferrofluid for continuous sheathless microfluidic separation of nonmagnetic microparticles

et al. 2016

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Separation of microparticles has found broad applications in biomedicine, industry and clinical diagnosis. In a conventional aqueous ferrofluid, separation of microparticles usually employs a sheath flow or two offset magnets to confine particle streams for downstream particle sorting. This complicates the fluid control, device fabrication, and dilutes the particle sample. In this work, we propose and develop a novel viscoelastic ferrofluid by replacing the Newtonian base medium of the conventional ferrofluid with non-Newtonian poly(ethylene oxide) (PEO) aqueous solution. The properties of both viscoelastic 3D focusing and negative magnetophoresis of the viscoelastic ferrofluid were verified and investigated. By employing the both properties in a serial manner, continuous and sheathless separation of nonmagnetic particles based on particle size has been demonstrated. This novel viscoelastic ferrofluid is expected to bring more flexibility and versatility to the design and functionality in microfluidic devices. Disciplines Engineering | Science and Technology Studies Abstract:Separation of microparticles has found broad applications in biomedicine, industry and clinical diagnosis. In a conventional aqueous ferrofluid, separation of microparticles usually employs a sheath flow or two offset magnets to confine particle streams for downstream particle sorting. This complicates the fluid control, device fabrication, and dilutes particle sample. In this work, we propose and develop a novel viscoelastic-based ferrofluid by replacing the Newtonian base medium of the conventional ferrofluid with non-Newtonian poly(ethylene oxide) (PEO) aqueous solution. The properties of both viscoelastic 3D focusing and negative magnetophoresis of the viscoelastic-based ferrofluid were verified and investigated. By employing the both properties in a serial manner, continuous and sheathless separation of non-magnetic particles based on particle size has been demonstrated. This novel viscoelastic ferrofluid is expected to bring more flexibility and versatility to the design and functionality in microfluidic devices.

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Continuous sheath-free magnetic separation of particles in a U-shaped microchannel

Cited by 43 publications

References 41 publications

Microfluidic bio-particle manipulation for biotechnology

Microfluidic bio-particle manipulation for biotechnology

Magnetophoretic-based microfluidic device for DNA isolation

A novel viscoelastic-based ferrofluid for continuous sheathless microfluidic separation of nonmagnetic microparticles

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