The importance of oxygen free radicals, iron and calcium in renal ischaemia

Jd, Gower; Cotterill, L; Green, C.J.

doi:10.1017/cbo9780511551482.011

Cited by 2 publications

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“…Removal of the blood supply to an organ results in the perturbation of a wide range of cellular and vascular homeostatic mechanisms. In addition to the over-production of oxygenderived free radicals, changes in cellular energy status, calcium redistribution, secondary messenger responses and production of vasoactive substances have all been implicated in ischaemia/reperfusion damage [33]. These events are interrelated in complex ways and it is most likely that a combination of adverse effects is responsible for delayed organ function upon engraftment.…”

Section: Discussionmentioning

confidence: 99%

The effect of a synthetic hexadentate iron chelator (CP130) and desferrioxamine on rabbit kidneys exposed to cold and warm ischaemia

et al. 1993

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The effect of CP130 (a synthetic hexadentate pyridinone iron chelator) on the formation of two markers of lipid peroxidation (TBA-reactive material and Schiff's bases) in rabbit kidneys following a 72 h period of cold (0-4 degrees C) ischaemia was investigated by either adding CP130 to the flush/storage solution (hypertonic citrate solution) or by administering the agent intravenously 15 min before removal of the organs. In both cases, CP130 blocked the adverse rises in lipid peroxidation caused by ischaemia and subsequent reoxygenation of the homogenates in vitro. Both CP130 and desferrioxamine (DFX) (administered intravenously 15 min before ischaemia and 5 min before reperfusion) were also found to significantly reduce post-ischaemic rates of in vitro lipid peroxidation in kidneys rendered warm ischaemic for 90 min followed by reperfusion for 5 or 60 min in situ. Kidneys exposed to warm ischaemia and reperfusion developed interstitial and intracellular oedema, congestion and haemorrhage. DFX administration had little effect on the histological outcome, whereas CP130 significantly reduced interstitial oedema (at 5 min reperfusion compared to the DFX-treated group), intracellular oedema (at 60 min reperfusion compared to the DFX-treated group) and congestion (at 5 min reperfusion compared with a control group not given any agent). It is concluded that while CP130 and DFX exhibited similar antioxidant properties, CP130 provided better protection from ischaemia/reperfusion injury at the histological level. Synthetic iron chelators may therefore be of benefit in clinical organ transplantation by protecting against tissue damage caused by prolonged ischaemia.

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Section: Discussionmentioning

confidence: 99%

The effect of a synthetic hexadentate iron chelator (CP130) and desferrioxamine on rabbit kidneys exposed to cold and warm ischaemia

et al. 1993

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“…LLC-PK1 cells were more markedly injured by hypoxia followed by reoxygenation than only by hypoxia as assessed by LDH leakage from the cells. Gower et al (13) have described that the rapid depletion of ATP levels compromises calcium pumps to result in a gain of cellular calcium levels in tissues subjected to ischemia-reperfusion. And this is likely to lead to derangement of cell function.…”

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confidence: 99%

Hypoxia and Reoxygenation-Induced Injury of Renal Epithelial Cells: Effect of Free Radical Scavengers

Yonehana

Gemba

1995

Japanese Journal of Pharmacology

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ABSTRACT-The aim of this study was to characterize injuries of LLC-PK1 and MDCK cells exposed to hypoxia and reoxygenation. Exposure of LLC-PK1 cells to hypoxia reduced the ATP contents and increased the leakage of lactate dehydrogenase (LDH), but MDCK cells had no such injuries. Hypoxia-reoxygenation of LLC-PK1 cells dramatically increased LDH leakage, which was suppressed by free radical scavengers, N N diphenyl p-phenylenediamine, superoxide dismutase and N. N dimethylthiourea. These results suggest that use of LLC-PK1 cells has advantages for the investigation of ischemia-reperfusion injury of the kidney as an in vitro model and that generation of oxygen radicals is involved in the cellular injury induced by hypoxia-reoxygenation.

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The importance of oxygen free radicals, iron and calcium in renal ischaemia

Cited by 2 publications

References 0 publications

The effect of a synthetic hexadentate iron chelator (CP130) and desferrioxamine on rabbit kidneys exposed to cold and warm ischaemia

The effect of a synthetic hexadentate iron chelator (CP130) and desferrioxamine on rabbit kidneys exposed to cold and warm ischaemia

Hypoxia and Reoxygenation-Induced Injury of Renal Epithelial Cells: Effect of Free Radical Scavengers

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