Hypoxia and Reoxygenation-Induced Injury of Renal Epithelial Cells: Effect of Free Radical Scavengers

Yonehana, Tsutomu; Gemba, Munekazu

doi:10.1254/jjp.68.231

Cited by 9 publications

(5 citation statements)

References 14 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The extent to which SOD or catalase are internalized has been questioned (Yonehana and Gemba, 1995;Doroshow 1986). In rat hepatocytes incubated with SOD, intracellular SOD activity was increased about 4-fold (Kyle et al, 1988).…”

Section: Extracellular Autoxidation and Pap Toxicitymentioning

confidence: 99%

“…The decrease in cell viability with DMTU was not due to toxicity of the scavenger itself since the toxicity was not seen in cells incubated with DMTU alone. DMTU has been used with opossum kidney cells (Park et al, 2003) and LLC-PK 1 cells (Yonehana and Gemba, 1995) with no evidence of toxicity. The interaction between PAP and DMTU is perplexing and will require further study.…”

Section: Intracellular Events In Pap Toxicitymentioning

confidence: 99%

See 1 more Smart Citation

Contribution of reactive oxygen species to para-aminophenol toxicity in LLC-PK1 cells

Foreman¹,

Tarloff²

2008

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

Para-aminophenol (PAP) causes nephrotoxicity by biochemical mechanisms that have not been fully elucidated. PAP can undergo enzymatic or non-enzymatic oxidation to form reactive intermediates. Using modulators of reactive oxygen species (ROS), the role of ROS in PAP toxicity in LLC-PK 1 cells was investigated. ROS formation was determined using a fluorescein derivative and viability using alamarBlue. Following treatment of cells with PAP, ROS formation occurred prior to loss of cell viability. Several modulators of ROS were used to identify the pathways involved in PAP toxicity. Viability was improved with catalase treatment, while viability was decreased when cells were treated with superoxide dismutase (SOD). Both catalase and SOD exert their effects outside of cells in the incubation medium, since there was no evidence of uptake of these enzymes in LLC-PK 1 cells. In cell-free incubations, hydrogen peroxide (H 2 O 2 ) was produced when 0.5 mM PAP was included in the incubation medium. Further, SOD greatly increased and catalase greatly decreased H 2 O 2 production in these cell-free incubations. These data suggest that H 2 O 2 formed in the incubation medium contributes to loss of viability following PAP treatment. When cells were coincubated with 0.5 mM PAP and tiron, pyruvate, bathocuproine, 1, 10-phenanthroline, or dimethylthiourea (DMTU), ROS formation was decreased. However, there was minimal improvement in cell viability. Paradoxically, DMTU exacerbated PAP-induced loss of viability. These data suggest ROS are generated in cells exposed to PAP but these species are not the predominant cause of cellular injury.

show abstract

Section: Extracellular Autoxidation and Pap Toxicitymentioning

confidence: 99%

Section: Intracellular Events In Pap Toxicitymentioning

confidence: 99%

Contribution of reactive oxygen species to para-aminophenol toxicity in LLC-PK1 cells

Foreman¹,

Tarloff²

2008

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

show abstract

“…Among these, it has been widely accepted that reactive oxygen species participate in the pathogenesis of ischemic acute renal failure (2,3). Therefore, investi gations of amelioration or prevention of cellular injury after renal ischemia through manipulation of free radi cal production have received much attention and indi cated that antioxidants have therapeutic potential (4,5).…”

mentioning

confidence: 99%

Attenuation of Renal Ischemia-Reperfusion Injury by Proanthocyanidin-Rich Extract from Grape Seeds

Nakagawa¹,

Yokozawa²,

Satoh³

et al. 2005

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

View full text Add to dashboard Cite

SummaryThe effects of proanthocyanidin-rich extract in rats subjected to renal ischemia-reperfusion were examined. Proanthocyanidin-rich extract, which is prepared from grape seeds (Vitis vinifera L.), was given orally at doses of 5 and 10mg/kg body weight/ d for 20 consecutive days prior to ischemia-reperfusion. Administration of proanthocyani din-rich extract attenuated renal dysfunction, as indicated by serum urea nitrogen and cre atinine levels. Additionally, in the ischemic-reperfused kidneys, increased levels of thiobarbituric acid (TBA)-reactive substance and alterations of antioxidant enzyme activi ties such as superoxide dismutase, catalase and glutathione peroxidase (GSH-Px) were observed. Proanthocyanidin-rich extract-treated groups showed significantly reduced renal TBA-reactive substance levels and enhanced catalase and GSH-Px activities, These results suggest that proanthocyanidin-rich extract has protective effects against ischemic-reperfu sion-induced renal damage associated with oxidative stress.

show abstract

“…Although no signi®cant suppression was observed within the tested concentration, the level of LDH leakage tended to decrease as the concentrations of caffeic acid, rosmarinic acid and lithospermic acid increased, suggesting that these compounds may have activity at higher concentrations. Yonehana & Gemba (1995) found that the intracellular level of the antioxidant glutathione was signi®cantly decreased under hypoxic conditions in LLC-PK 1 cells. Snowdowne et al (1985) and Kribben et al (1994) reported that hypoxia caused an increase of intracellular calcium in a kidney-derived cultured cell line, LLC-MK 2 , and also in isolated renal tubules.…”

Section: Discussionmentioning

confidence: 91%

Evaluation of Caffeic Acid Analogues using a Cultured Renal Epithelial Cell Line, LLC-PK<SUB>1</SUB>

Yokozawa¹,

Liu²,

Chen³

et al. 1999

Pharmacy and Pharmacology Communications

View full text Add to dashboard Cite

The effects of several caffeic acid analogues isolated from Salviae Miltiorrhizae Radix (Salvia miltiorrhiza Bunge) on the cultured renal epithelial cell line, LLC‐PK1, were studied. Incubation of the cells with different concentrations of these compounds for 48 h markedly suppressed lactate dehydrogenase leakage, which was elicited when the cells were exposed to hypoxia/re‐oxygenation or cephaloridine. The most active compounds were magnesium lithospermate B and lithospermic acid B, two tetramers of caffeic acid, followed by lithospermic acid (trimer) and rosmarinic acid (dimer). Caffeic acid showed poor activity. These results indicate that caffeic acid analogues, in particular magnesium lithospermate B and lithospermic acid B, exert a direct protective effect on renal cells.

show abstract

Hypoxia and Reoxygenation-Induced Injury of Renal Epithelial Cells: Effect of Free Radical Scavengers

Cited by 9 publications

References 14 publications

Contribution of reactive oxygen species to para-aminophenol toxicity in LLC-PK1 cells

Contribution of reactive oxygen species to para-aminophenol toxicity in LLC-PK1 cells

Attenuation of Renal Ischemia-Reperfusion Injury by Proanthocyanidin-Rich Extract from Grape Seeds

Evaluation of Caffeic Acid Analogues using a Cultured Renal Epithelial Cell Line, LLC-PK<SUB>1</SUB>

Contact Info

Product

Resources

About