The Importance of Light Intensity for Pollen Tube Growth and Embryo Survival in Wheat × Maize Crosses

Campbell, A. G.; Griffin, W. B.; Burritt, David J.; Conner, A. J.

doi:10.1006/anbo.2000.1363

Cited by 25 publications

(19 citation statements)

References 31 publications

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“…To simplify the operation, we changed the approach of 2,4-D treatment from injecting or/and spraying ear by ear (Laurie, 1989) to spraying the collected cut plants (24 h after pollination). Therefore, cutting too late may leave the hybrid spikes short of enough 2,4-D supply because the plant itself can only make limited 2,4-D. On the other hand, the decrease in embryo rate when cutting plants 4 h after pollination was possibly related to the interference of elongation of pollen tubes or fertilization in parts of florets (Zhang et al, 1996;Campbell et al, 2001). In addition, the formula of culture solution for cut plants is another important factor influencing the efficiency of haploid embryo induction.…”

Section: Discussionmentioning

confidence: 97%

Study on the in vitro culture of cut plants in wheat haploid embryo induction by a wheat × maize cross

Liu

et al. 2008

Front. Agric. China

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Study on the in vitro culture of cut plants in wheat haploid embryo induction by a wheat 6 maize cross E Higher Education Press and Springer-Verlag 2008Abstract The wheat 6 maize system is one of the most effective ways to produce haploids in wheat. Whether and how it could be successfully applied in practical breeding mostly depends upon the efficiency of haploid embryo production. To perfect the protocols of haploid embryo induction, the efficiency of haploid embryo production between in vitro culture of cut plant and intact plant growth for hybrid spikes with two F 1 wheat hybrids and two maize varieties was compared. Effects of different cutting plant times and formulas of nutrient solutions for cut plant culture on haploid embryo formation were also studied. Results indicated that the embryo rate of in vitro culture was 3.29 times that of intact plant growth, with the figures of 31.6% vs 9.6%, respectively. The optimal time for cut plant culture was 24 h after pollination. Formulas of nutrient solutions significantly affected the efficiency of haploid embryo induction. With an embryo rate of 0-35.5%, adding calcium phosphate in the culture solution at 3 g?L 21 could raise the caryopsis and embryo rates. According to this study, the best medium for cut plant culture was: 100 mg?L 21 2,4-D + 40 g?L 21 sucrose + 10 mg?L 21 silver nitrate + 8 mL?L 21 sulfurous acid + 3 g?L 21 calcium phosphate, with which a caryopsis rate of 95% and an embryo rate of about 30% could be obtained.Keywords wide cross between wheat and maize, in vitro culture of cut plants, haploid embryo induction

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Section: Discussionmentioning

confidence: 97%

Study on the in vitro culture of cut plants in wheat haploid embryo induction by a wheat × maize cross

Liu

et al. 2008

Front. Agric. China

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“…Considering GA is required for the pollen tube growth (Singh et al, 2002), it is tempting to suppose that the environmental factors, such as light and temperature, play an important role in pollination/fertilization. In fact, the light intensity and temperature have a profound effect on the pollen tube growth and embryo survival in some species (Campbell et al, 2001;Young et al, 2004). In this study, 24 cDNAs were both responsive to GA and pollination/fertilization ( Figure S1) and their further functional analysis will help shed light on the GA response pathway during this important biological process.…”

Section: Ga Response Pathway In Pollination/fertilization In Ricementioning

confidence: 92%

Microarray Analysis Reveals Similarities and Variations in Genetic Programs Controlling Pollination/Fertilization and Stress Responses in Rice (Oryza sativa L.)

Lan

Lai

et al. 2005

Plant Mol Biol

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Previously, we identified 253 cDNAs that are regulated by pollination/fertilization in rice by using a 10K cDNA microarray. In addition, many of them also appeared to be involved in drought and wounding responses. To investigate this relationship, we obtained their expression profiles after dehydration and wounding treatments in this study. Venn diagram analysis indicated that 53.8% (136/253) and 21% (57/253) of the pollination/fertilization-related genes are indeed regulated by dehydration and wounding, respectively, and nearly half of the genes expressed preferentially in unpollinated pistils (UP) are responsive to dehydration. These results indicated that an extensive gene set is shared among these responses, suggesting that the genetic programs regulating them are likely related. Among them, the genetic network of water stress control may be a key player in pollination and fertilization. Additionally, 39.5% (100/253) cDNAs that are related to pollination/fertilization appear not to be regulated by the stress treatments (dehydration and wounding), suggesting that the existence of additional genetic networks are involved in pollination/fertilization. Furthermore, comparative analysis of the expression profiles of the 253 cDNAs under 18 different conditions (various tissues, treatments and developmental status) revealed that the genetic networks regulating photosynthesis, starch metabolisms, GA- and defense-responses are involved in pollination and fertilization. Taken together, these results provided some clues to elucidate the molecular mechanisms of pollination and fertilization in rice.

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“…These authors found that not only light intensity but also the genotype had an influence on embryo recovery, however, embryo recovery was higher under the highest light intensity. In the study of Campbell et al (2001), haploid embryo survival of the cultivar Karamu was significantly affected by the light intensity, as 34% of pollinated florets produced haploid embryos at the high light intensity (750 μmol m -2 s -1 ) compared to only 4% of pollinated florets that produced haploid embryos at the low light intensity (250 μmol m -2 s -1 ). Cultivar Kotuku was unaffected by different light intensities, as 33% of pollinated florets produced haploid embryos at the high light intensity and 38% at the low light intensity.…”

Section: Discussionmentioning

confidence: 99%

The effect of light intensity on the production of oat (Avena sativaL.) doubled haploids through oat × maize crosses

Skrzypek

Warchoł

Czyczyło-Mysza

et al. 2016

Cereal Research Communications

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Oat haploid embryos were obtained by wide crossing with maize. The effect of light intensity during the growing period of donor plants (450 and 800 μmol m -2 s -1 ) and in vitro cultures (20, 40, 70 and 110 μmol m -2 s -1 ) was examined for the induction and development of oat DH lines. Oat florets (26008) from 32 genotypes were pollinated with maize and treated with 2,4-dichlorophenoxyacetic acid. All the tested genotypes formed more haploid embryos when donor plants were grown in a greenhouse (9.4%) compared to a growth chamber (6.1%). The light intensity of 110 μmol m -2 s -1 during in vitro culture resulted in the highest percentage of embryo germination (38.9%), conversion into plants (36.4%) and DH line production (9.2%) when compared with lower light intensities (20, 40 and 70 μmol m -2 s -1 ). The results show that the growth conditions of the donor plant and light intensity during in vitro culture can affect the development of haploid embryos. This fact may have an impact on oat breeding programs using oat × maize crosses.

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The Importance of Light Intensity for Pollen Tube Growth and Embryo Survival in Wheat × Maize Crosses

Cited by 25 publications

References 31 publications

Study on the in vitro culture of cut plants in wheat haploid embryo induction by a wheat × maize cross

Study on the in vitro culture of cut plants in wheat haploid embryo induction by a wheat × maize cross

Microarray Analysis Reveals Similarities and Variations in Genetic Programs Controlling Pollination/Fertilization and Stress Responses in Rice (Oryza sativa L.)

The effect of light intensity on the production of oat (Avena sativaL.) doubled haploids through oat × maize crosses

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