The importance of explant source on regeneration and micropropagation of Gladiolus by liquid shake culture

Reduced temperature, elevated sucrose, continuous light and gibberellic acid promote corm formation in Watsonia vanderspuyiae

“…Major variables associated with this treatment are the extent of reduction and the duration of exposure . For example, temperatures are effective from 4°C for Hyacinthus (Bach et al 1992), 10°C for Lachenalia (Slabbert and Niederwieser 1999), 15°C for Sparaxis (Hauser and Horn 1991) and 20°C for Gladiolus (Nhut et al 2004). In some species, there is a definitive temperature above which storage organ formation is completely inhibited.…”

“…In some species, there is a definitive temperature above which storage organ formation is completely inhibited. For example, this occurs at 21°C in Lachenalia, (Slabbert and Niederwieser 1999), 25°C in Gladiolus (Nhut et al 2004), and27°C in Nerine (Jacobs et al 1992). In the case of Crinum macowanii, bulblet formation was inhibited at 15°C, but promoted at high temperatures of 25 and 30°C (Slabbert et al 1993).…”

Micropropagation of iridaceae—a review

“…The summer-flowering G. dalenii formed corms at 24°C, while the winter-flowering G. tristis formed corms at 15°C (De Bruyn and Ferreira 1992). Shoots of G. grandzjlorus formed corms better at 15 and 20°C, compared to those grown at 25°C (Nhut et al 2004). In terms of growth regulators BA, kinetin, ABA and GA 3 inhibited corm induction in a static liquid medium (Dantu and Bhojwani 1995).…”

“…Plantlets rooted on high sucrose had greater survival than those rooted on media with normal (3%) sucrose levels (Kumar et al 1999). Explant source is critical in any tissue culture procedure, and for Gladiolus, and most of the Iridaceae, the most popular tissue source has been corms (Nhut et al 2004) or inflorescence explants (Ziv and Lilien-Kipnis 2000).…”

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

“…The frequency of microplants, on the other hand, is largely determined by a number of parameters, including the type of explants employed, genotype, composition of culture medium, and PGR types and concentration (Figure 1 and Supplementary Table S1). Direct organogenesis have demonstrated by researchers utilizing a variety of explants, including corms or inflorescence explants, axillary buds basal leaf tissues, meristem, explants from inflorescence stalk, and various other explants such as shoot tips or cormel sprouts, cormels, and cormel segments [8,9,[26][27][28]38,51,[58][59][60].…”