Reduced temperature, elevated sucrose, continuous light and gibberellic acid promote corm formation in Watsonia vanderspuyiae

Ascough, G.D.; Erwin, John E.; Staden, J. Van

doi:10.1007/s11240-008-9441-6

Cited by 17 publications

(10 citation statements)

References 37 publications

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“…Subsequent experiments lasting 6 months revealed that reduced culture temperature induced corm formation in W. lepida, and elevated sucrose levels, GA 3 and continuous light all promoted corm induction in W. vanderspuyiae (Ascough et al 2008c). A factorial experiment examining the interaction of these four factors showed that GA 3 and reduced temperature (20°C) acted synergistically to promote corm formation and growth.…”

Section: Watsoniamentioning

confidence: 95%

Micropropagation of iridaceae—a review

Ascough

Erwin

Staden

2009

Plant Cell Tiss Organ Cult

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The Iridaceae contains many hundreds of attractive species that have been overlooked for horticultural development. The incredible variation in flower and leaf shape, size and colour suggest this little-tapped resource could offer great opportunities for developing new commercial ornamental products. Micropropagation has increasingly become a valuable tool assisting breeders to release new species and cultivars into the market more rapidly. Here we review the progress made in Iridaceae micropropagation genus by genus, and highlight the potential for future expansion in this field.

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Section: Watsoniamentioning

confidence: 95%

Micropropagation of iridaceae—a review

Ascough

Erwin

Staden

2009

Plant Cell Tiss Organ Cult

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“…We did not observe any significant enhancement in frequency of tuberization, NTPs and frequency of proliferation in PBZ treatment; however, its positive effects have been observed in some other species (Balamani and Poovaiah 1985;Ziv 1990). Generally, facilitating carbohydrate supply enhances the formation and growth of storage organs (Ascough et al 2008). Jasik and Mantell (2000) observed higher microtuber numbers and larger microtuber size of D. cayenensis after using 40 g l -1 sucrose instead of 20 g l -1 .…”

Section: Resultsmentioning

confidence: 99%

“…Shortened shoots with various abnormal structures cultured in the medium containing 80 g l -1 sucrose; c shortened shoot with aerial bulbils on two or three axillary buds; d shortened shoot with aerial bulbil on upper axillary bud, and microtuber on basal axillary bud; e shortened shoot only with microtuber on basal axillary bud; f ex vitro rooted plantlet with minituber at the base of shoot. MT Microtuber, AB aerial bulbil that leads to development in storage organ as an avoidance response to a perceived unfavorable climate (Ascough et al 2008).…”

Section: Resultsmentioning

confidence: 99%

Axillary shoot proliferation and tuberization of Dioscorea fordii Prain et Burk

Yan

Yang

2010

Plant Cell Tiss Organ Cult

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Dioscorea fordii Prain et Burk., a member of the family Dioscoreaceae, is an important tuberous food in China owing to its edible and medicinal functions. However, the lack of healthy planting material has restricted its production. The present study was an attempt to develop a protocol for in vitro propagation of D. fordii. In an initial two by two (2 2 ) factorial experiment, the effects of culture system and activated charcoal have been observed for axillary shoot proliferation and tuberization of in vitro plantlets. Shoot length, frequency of proliferation, fresh weight and dry weight of shoots, frequency of tuberization and mean number of tubers per plantlet (NTPs) were significantly (P B 0.05) greater in liquid medium compared to semi-solid media. Secondly, an orthogonal experimental design [L 9 (3 4 )] was used to investigate the effects of the ratio of naphthalene acetic acid (NAA) to 6-benzyladenine (BA) (NAA/BA), paclobutrazol, methyl jasmonate and sucrose concentration on tuberization. Sucrose concentration had the record effect on frequency of tuberization, NTPs and frequency of proliferation. The preferred medium for axillary shoot proliferation and tuberization of D. fordii found to be MS basal medium (Murashige and Skoog in Physiol Plant 15:473-479, 1962) supplemented with 1.0 mg l -1 BA, 0.1 mg l -1 NAA, 30 g l -1 sucrose and 1.5 g l -1 AC in liquid culture.

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“…The increase of the sucrose concentration in the medium stimulated the lily and onion bulb formation (Gerrits and De Klerk, 1992; Kastner et al, 2001;Keller, 1993;Takayama and Misawa, 1980), the corm induction of Watsonia vanderspuyiae (Ascough et al,. 2008), the microtubers formation of Solanum tuberosum (Gopal et al, 2001), Xanthosoma sagittifolium (Omokolo et al, 2003), Dioscorea cayenensis -D. rotundata complex (Ovono et al, 2009) and the microrhizome production of Zingiber officinale (Sharma et al, 1997).…”

Section: Effects Of Different Culture Vessels On Microrhizome Productionmentioning

confidence: 99%

Silver Nitrate and Different Culture Vessels Influence High Frequency Microrhizome Induction In Vitro and Enhancement Growth of Turmeric Plantlet During Ex Vitro Acclimatization

et al. 2012

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Eleven cultivars of C. longa var. Lakadong were collected from Manipur having different topography. Curcumin content in different cultivars has been analyzed by using UV-Visible Spectrophotometer (100 Bio-Carry Spectrophotometer). The curcuminoids content were analyzed and quantified for identification of best quality cultivar. Thoubal Cultivar with highest curcumin content (9.44%) was subjected for tissue culture technique using different culture vessels and silver nitrate for rapid multiplication and scaling up of microrhizome production. High multiplication rate of 27.40±0.47 were obtained in Murashige and Skoog's medium supplemented with 3% sucrose + 1 mg L -1 α-napthalene acetic acid, 4 mg L -1 6-benzyl-amino-purine and 11 µM silver nitrate. Effect of different culture vessels and silver nitrate were studied for microrhizome and multiple shoots formation. Relatively higher rate of shoots along with microrhizome (17.5±0.32) can be seen in Growtek which was grown without any plant growth regulator. Growtek was used for scaling up of microrhizome production in vitro and utmost microrhizome was produced in liquid Murashige and Skoog's medium supplemented with 8% sucrose, 1 mg L -1 α-napthalene acetic acid, 4 mg L -1 6-benzyl-amino-purine and 11 µM silver nitrate (36.25±0.27). Addition of silver nitrate in the medium resulted in improvement of microrhizome induction in vitro. Higher concentration of silver nitrate (33, 44, 66, 88 µM) negatively affected the microrhizome and shoot multiplication and shows inhibition of tissue response completely. Analysis of in vitro derived plantlets during acclimatization shows that the exogenous applied of silver nitrate shows superior growth as compared to control. 90-95% of plantlets with and 75-80% plantlets without silver nitrate treatment were successfully established under ex vitro acclimatization. The protocol could be utilized for large scale production of true-to-type plantlets and as alternative method to step forward towards an improved commercial propagation system for more efficient and productivity.

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Reduced temperature, elevated sucrose, continuous light and gibberellic acid promote corm formation in Watsonia vanderspuyiae

Cited by 17 publications

References 37 publications

Micropropagation of iridaceae—a review

Micropropagation of iridaceae—a review

Axillary shoot proliferation and tuberization of Dioscorea fordii Prain et Burk

Silver Nitrate and Different Culture Vessels Influence High Frequency Microrhizome Induction In Vitro and Enhancement Growth of Turmeric Plantlet During Ex Vitro Acclimatization

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