The impact of Tunisian Capparidaceae species on cytological, physiological and biochemical mechanisms in lettuce

Ladhari, Afef; Omezzine, Faten; Haouala, Rabiaa

doi:10.1016/j.sajb.2014.04.014

Cited by 19 publications

(14 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…According to our previous studied [ 3 , 14 ] we have speculated that the inhibitory effect of the C. arabica extracts could be mediated induction of oxidative stress and ROS generation in target species. To address this possibility, we next determined the extent of ROS generation quantitatively in terms of hydrogen peroxide content in lettuce plant under C. arabica extract and its identified compounds.…”

Section: Resultsmentioning

confidence: 99%

“…Since there are very few studies on the identified active compounds form C. arabica , it is crucial to continue discovering new bioactive allelochemicals from the most active plant organ [ 3 ] that could promote new target site of action. Correlating the phytotoxicity assays with the physiological effect could understand deeper the mechanism of action of the active compound of C. arabica [ 14 ]. Actually, there are no work in the literature that correlate the main phytotoxic effects of C. arabica compounds with cellular events related to physiological, cytological, and antioxidant enzymes activities.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Physiological and Oxidative Stress Responses of Lettuce to Cleomside A: A Thiohydroximate, as a New Allelochemical from Cleome arabica L.

2020

Self Cite

View full text Add to dashboard Cite

The inclination toward natural products have led the onset for the discovery of new bioactive metabolites that could be targeted for specific therapeutic or agronomic applications. This study aimed to isolate bioactive compounds from Cleome arabica L., and subsequently determine the unexplored mechanism of action of the newly identified compounds on Lactuca sativa L. Chemical investigation of the ethyl acetate fraction of methanolic silique extract of C. arabica afforded seven secondary metabolites belonging to different classes such as flavonoids, triterpene, and a new thiohydroximate derivative, named cleomside A. Among phytotoxic assays, the growth of lettuce was totally inhibited by cleomside A compared to the other identified compounds. This effect was associated with the increased levels of electrolyte leakage, malondialdehyde, and hydrogen peroxide indicating disruption of membrane integrity and induction of oxidative stress. Activities of the antioxidant enzymes SOD, CAT, and APX were also elevated, thereby demonstrating the enhanced generation of reactive oxygen species upon identified allelochemical exposure. Thus, the changes caused by cleomside A described herein can contribute to better understanding the allelochemical actions of thiohydroximate and the potential use of these substances in the production of natural herbicides compared to the other identified flavonoids and triterpene.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Physiological and Oxidative Stress Responses of Lettuce to Cleomside A: A Thiohydroximate, as a New Allelochemical from Cleome arabica L.

2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Seedling growth is known to depend on DNA synthesis, mitotic divisions and seed reserve mobilization (Cardoso 2004, Bewley et al 2013. Mitotic divisions can be reduced by the action of allelochemicals (Ladhari et al 2014), compromising the normal plant development. Romagni et al (2000) reported that the compound 1,8-cineol is present as a mitotic index and root growth reducer.…”

Section: Resultsmentioning

confidence: 99%

Physiological and biochemical effects of Lantana camara L. allelochemicals on the seed germination of Avena sativa L.

Gindri

Coelho

Uarrota

2020

Pesqui. Agropecu. Trop.

View full text Add to dashboard Cite

Lantana camara (L.) is considered one of the main weeds of agricultural crops, and allelochemicals produced by this species may be a source for the development of natural herbicides. In this study, phytotoxic effects of L. camara aqueous extracts on seed germination and seedling growth of Avena sativa (L.) were assessed. The experimental design was completely randomized, in a 2 x 5 factorial scheme, with treatments resulting from two assays, testing five concentrations of the extract (0 %, 2.5 %, 5.0 %, 7.5 % and 10 %; vv-1). During the seed germination, physiological (e.g., seed imbibition, germination and vigor) and biochemical (e.g., catalase, ascorbate peroxidase, proline, phenols, malondialdehyde and hydrogen peroxide) proprieties were evaluated. The leaf extracts of L. camara affected the response variables on A. sativa by reducing the germination speed index (phases I and II) and seed viability (phase III of germination), thus causing a solute leakage and increasing the production of reactive oxygen forms and, finally, lipid peroxidation. Extract concentrations above 2.5 % inhibit the growth of epicotyls. Therefore, the studied allelochemicals showed potential to be exploited in the development of natural herbicides.

show abstract

“…Jana & Biswas (2011) isolaram das raizes de Cleome viscosa o ácido lacto nonanoico, responsável pela ação inibitória dessa planta sobre sementes de arroz, mostarda e de grama. Da mesma forma, o extrato metanólico das vagens de Cleome arabica exerceu um potente efeito negativo na germinação das sementes de alface, bem como no crescimento dessas plântulas, devido a danos celulares na raiz correlacionados a redução mitótica (Ladhari et al, 2014). Portanto, sugere-se que algumas espécies do gênero Cleome concorram pela sobrevivência no ambiente liberando aleloquímicos.…”

Section: Resultado E Discussãounclassified

Efeito fitotóxico do óleo volátil de Cleome guianensis Aubl. sobre o crescimento inicial de Senna occidentalis L.

Ventura

Silva

Simionatto

et al. 2016

Rev. bras. plantas med.

View full text Add to dashboard Cite

RESUMO:Aleloquímicos são substâncias liberadas por certas espécies vegetais no ambiente que influenciam a germinação e o desenvolvimento de outras plantas. O objetivo desse estudo foi avaliar o potencial fitotóxico de Cleome guianensis Aubl., bem como determinar o grau de estresse oxidativo causado em Senna occidentalis L. Para isso, o óleo das folhas de C. guianensis, nas concentrações 0,5 µg/mL, 1,0 µg/mL, 2,5 µg/mL e 5,0 µg/mL, em solução de 5 ml foi testado em bioensaios de germinação e de crescimento da raiz primária e do hipocótilo de S. occidentalis. Os indivíduos de S. occidentalis foram macerados, homogeneizados e submetidos a ensaios de quantificação da catalase, peroxidase, SOD e de peroxidação lipídica. Os resultados indicam que todas as concentrações do óleo volátil causaram um efeito negativo sobre a germinação e crescimento de S. occidentalis. Na concentração 5,0 µg/mL, houve 56% de inibição da germinação e 83% de inibição de crescimento do hipocótilo. Ainda, o óleo aumentou a atividade das enzimas induzidas pelo estresse oxidativo catalase, peroxidase e superóxido dismutase (SOD). Quanto à peroxidação lipídica, o óleo nas concentrações 1,0 µg/ mL, 2,5 µg/mL e 5,0 µg/mL aumentou a produção de malondialdeído. Sendo assim, conclui-se que C. guianensis apresenta aleloquímicos que influenciam na germinação e no crescimento de S. occidentalis, além de aumentar a atividade das enzimas catalase, peroxidase, SOD e a produção de malondialdeído em S. occidentalis. Dessa forma, sugere-se a realização de estudos sobre o perfil químico do óleo a fim de descobrir as substâncias responsáveis por tais resultados e consolidar o potencial fitotóxico de C. guianensis.Palavras-chave: Alelopatia, bioherbicida, estresse oxidativo.ABSTRACT: Phytotoxic effect of Cleome guianensis Aubl. on the initial growth of Senna occidentalis L. Allelochemicals inhibit the germination and growth of other plants. The purpose of this study was to assess the phytotoxic potential of Cleome guianensis Aubl., as well as determine the degree of oxidative stress caused in Senna occidentalis L. For this purpose, oil from the leaves of C. guianensis in concentrations of 0.5 µg/mL, 1.0 µg/mL, 2.5 µg/mL, and 5.0 µg/mL were tested in bioassays on the germination and growth of S. occidentalis. Specimens of S. occidentalis were macerated, homogenized, and submitted to catalase, peroxidase, superoxide dismutase (SOD), and lipid peroxidation quantification tests. Results showed that all concentrations of the volatile oil had a negative effect on S. occidentalis germination and growth. In the 5.0 µg/mL concentration, the oil inhibited 56% of germination and 83% of hypocotyl growth. In addition, the oil increased the activity of the enzymes induced by oxidative stress: catalase, peroxidase, and superoxide dismutase SOD. For lipid peroxidation, the oil in the 1.0 µg/mL, 2.5 µg/mL and 5.0 µg/mL concentrations increased the production of malondialdehyde. In addition, C. guianensis presents allelochemicals that influence the germination and growth of S. occ...

show abstract

The impact of Tunisian Capparidaceae species on cytological, physiological and biochemical mechanisms in lettuce

Cited by 19 publications

References 57 publications

Physiological and Oxidative Stress Responses of Lettuce to Cleomside A: A Thiohydroximate, as a New Allelochemical from Cleome arabica L.

Physiological and Oxidative Stress Responses of Lettuce to Cleomside A: A Thiohydroximate, as a New Allelochemical from Cleome arabica L.

Physiological and biochemical effects of Lantana camara L. allelochemicals on the seed germination of Avena sativa L.

Efeito fitotóxico do óleo volátil de Cleome guianensis Aubl. sobre o crescimento inicial de Senna occidentalis L.

Contact Info

Product

Resources

About