The impact of supplemental carbon sources on Arabidopsis thaliana growth, chlorophyll content and anthocyanin accumulation

Abstract

This research explores the impacts of a broad range of supplemental carbon sources on growth and development of Arabidopsis thaliana. Parameters measured include dark-germinated hypocotyl length, light-germinated root growth, rosette growth, chlorophyll concentration and anthocyanin content. Treatment sugars include sucrose, maltose, D-glucose, D-fructose, L-arabinose, L-fucose, D-galactose, D-mannose, L-rhamnose and D-xylose each supplied at 4, 20 or 100 mM. This comparison of the effect of different carbon sources on multiple parameters and under identical conditions showed that every carbon source had unique qualitative and quantitative effects on Arabidopsis growth and development. Root growth was particularly sensitive to supplemental carbon source. Growth on 100 mM sucrose, maltose, glucose or xylose stimulated root growth by *100%. Growth on arabinose, fucose, galactose, mannose or rhamnose inhibited root growth by 50% or more. Several sugars that strongly inhibited root growth had either no effect (galactose and fucose) or a positive effect (arabinose) on hypocotyl elongation and rosette growth. Rhamnose was the only carbon source that inhibited hypocotyl elongation across all concentrations. Sucrose, maltose, glucose, fructose, arabinose or xylose stimulated rosette growth by *50%. Chlorophyll content was strongly reduced by mannose while sucrose, glucose, galactose and rhamnose caused smaller reductions. Anthocyanin accumulation was strongly induced by both galactose and mannose. Only mannose impacted all parameters across all concentrations. Based on these data it can be concluded that the effect of each carbon source on Arabidopsis growth and development is specific in terms of both magnitude and the parameters impacted.

Cellulose Deficiency Is Enhanced on Hyper Accumulation of Sucrose by a H+-Coupled Sucrose Symporter

“…2A). This result is consistent with a previous report that systematically investigated the effect of various carbon sources on the growth of wild-type Arabidopsis (Stevenson and Harrington, 2009). However, we observed that shv3svl1 exhibited a more dramatic response to Suc, with no other sugars inhibiting hypocotyl elongation and Fru slightly increasing hypocotyl elongation (Fig.…”

Pseudomonas fluorescens Transportome Is Linked to Strain-Specific Plant Growth Promotion in Aspen Seedlings under Nutrient Stress

“…Molecules with potential transporters in the Pseudomonas transportomes that have been shown to influence root morphology include several of the sugars (Baskin et al, 2001; Stevenson and Harrington, 2009), polyamines (Couee et al, 2004), and glutamate (Walch-Liu and Forde, 2007). These molecules and related compounds could be produced and released by Pseudomonas or alternatively produced by the plant and taken up and utilized by Pseudomonas .…”

Interplay between Sucrose and Folate Modulates Auxin Signaling in Arabidopsis

“…The effect of sugars on development can be seen across all stages of the plant life cycle, from germination and establishment (Zhou et al, 1998;Rognoni et al, 2007) through vegetative growth and floral transition (Ohto et al, 2001;Corbesier et al, 2002) until senescence (Wingler and Roitsch, 2008). At the earliest stages of plant growth, the perception of sugars influences a number of seedling traits, including primary root growth (Freixes et al, 2002;Yazdanbakhsh et al, 2011) and hypocotyl elongation (Stevenson and Harrington, 2009). As carbohydrate availability fluctuates on a diurnal cycle (Bläsing et al, 2005), other metabolic and environmental stimuli are in flux as well (Gibon et al, 2006), and it is therefore necessary for sugar signals to be integrated with other signaling pathways to promote the appropriate developmental responses.…”