The Impact of Sub Acute Administration of Purified Gambier (Uncaria gambir Roxb.) to The Liver and Kidney Functions and its Reversibility on Rats

Armenia,; Permatasari, Dita; Sinamar, Lathifah Putri; Estera, Keke; Ahmadin, Almahdy

doi:10.5530/pj.2021.13.7

Cited by 6 publications

(4 citation statements)

References 4 publications

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“…The creatinine clearance of the rats in this study was not significantly affected by the administration of GE. This result was consistent with several previous studies where there was no significant difference in creatinine clearance after administering GE to rats for 14 days ( 16 , 17 , 18 ). Our study monitored the rats for 60 days, and the results still showed no significant difference in creatinine clearance.…”

Section: Discussionsupporting

confidence: 93%

Comparison of Gambier Extract (Uncaria Gambier Robx) and Angiotensin Receptor Blocker on Proteinuria Reduction and Antioxidants - Enhancement in Nephrotic Rat Models

Siregar,

Ramayani,

Haris

et al. 2023

Med Arch

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Background: Proteinuria is a significant clinical manifestation that causes edema in several diseases, including Nephrotic Syndrome (NS). Untreated proteinuria is strongly linked to the progression of kidney failure. One of the adjuvant therapies could be used to reduce proteinuria such as Angiotensin Receptor Blocker (ARB) including Losartan®. Gambier is a traditional medicinal plant widely known for its antioxidant effects. Catechin, a compound contained in Gambier Extract (GE), has been used to reduce microalbuminuria in diabetics. However, its application in NS has not been widely studied. Objective: This study compared the effects of GE and ARB in reducing proteinuria and increasing antioxidant activity levels, as well as reported histopathological findings in the nephrotic Wistar rat model. Methods: An experimental design study with a control group and a posttest was conducted. The experimental animals were divided into four groups: the control group (K1), the group with puromycin aminonucleoside (PAN) injection (K2), the group with PAN injection + GE (K3), and the group with PAN injection + Losartan® (K4). The standard GE used was Sarie Uncariae® by Toyo Brothers, PT while the ARB (Losartan®) was obtained from Novell, PT. Protein urine, the activity level of total superoxide dismutase (T-SOD), and malondialdehyde (MDA) were assessed using the colorimetric method. Renal histopathology was assessed based on Rollerman’s criteria. Results: Gambier extract significantly reduced proteinuria, as depicted by a decrease in protein/volume urine (p = 0.009), increased antioxidant activity, as illustrated by an elevation in T-SOD activity levels (p = 0.007), and tended to decrease MDA levels compared to Losartan®. Based on histopathological findings, GE tended to reduce the percentage of kidney damage in rats induced by puromycin. Conclusion: Gambier extract has been shown a higher antioxidant effect by increasing T-SOD activity levels, reducing proteinuria and also exhibiting a tendency to diminish kidney damage.

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Section: Discussionsupporting

confidence: 93%

Comparison of Gambier Extract (Uncaria Gambier Robx) and Angiotensin Receptor Blocker on Proteinuria Reduction and Antioxidants - Enhancement in Nephrotic Rat Models

Siregar,

Ramayani,

Haris

et al. 2023

Med Arch

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“…In this study, PG 5 and 10 experienced hyperfiltration until the third day after administration of the preparation; this is in accordance with previous research by Armenia et al, (2021), which states that gambir at a dose of 5-20 mg can cause hyperfiltration characterised by the percentage value of renal function exceeding 100% [3]. However, this treatment group experienced a decrease in creatinine clearance until day 14.…”

Section: Discussionsupporting

confidence: 92%

“…Uncaria gambir from the Rubiaceae family has various activities, including antioxidant [10], anti-inflammatory [11], antiatherosclerosis [12] and hypoglycemic effect. Previous research revealed that purified gambier reduce blood sugar levels and blood pressure in diabetic-hypertensive rats [13].…”

Section: Introductionmentioning

confidence: 99%

Purified Gambir (Uncaria Gambir (Hunter) Roxb.) Attenuate Renal Function in Alloxan-Induced Diabetic Male Rats

RACHMAINI,

ARMENIA,

ABDILLAH

2024

Int J App Pharm

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Objective: Diabetes and its vascular complications could prevent by antioxidants. Purified gambir is the purified extract of Uncaria gambir (Hunter) Roxb, which contains more than 90% catechins and scientifically work as antioxidant. This study aimed to determine the effect of purified gambir on renal function in alloxan-induced diabetic Wistar Kyoto rats. Methods: Diabetic rats were induced by alloxan 125 mg/kgBW by intraperitoneal injection. Forty-five diabetic rats were divided into five groups, diabetic control group treated with vehicle, a positive control group treated with glibenclamide at dose 0.45 mg/kgBW, and treatment groups of purified gambir at doses of 2.50, 5, and 10 mg/kgBW for 14 d. Blood glucose, water intake, urine volume, creatinine clearance, renal ratio, and urine density were measured on day 3, 7, and 14. All data were analyzed by two-way ANOVA followed by Duncan Multiple Range Test. Results: Purified gambir significantly lower blood glucose (p<0.05) and had a significant effect (p<0.05) on renal function parameters in diabetic rats. Duration of treatment purified gambir in diabetic rats had a significant effect (p<0.05) on water intake, urine volume, creatinine clearance and renal ratio. Conclusion: This result show that purified gambir at dose 2.5 mg/kgBW increased the renal function of diabetic rats, in contrast, purified gambir at dose 5 and 10 mg/kgBW attenuate renal function of diabetic rats

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“…Dari penelusuran literatur, telah dilaporkan adanya penelitian aktifitas gambir sebagai analgetik dan antiinflamasi (Sari, 2010), antimikroba, antioksidan dan antinematoda (Isnawati et al, 2012). Penelitian tentang toksisitas terhadap fungsi ginjal dan hati telah dilakukan sebelumnya (Armenia, et al, 2021).…”

Section: Pendahuluanunclassified

The Sub Acute Toxicity Study of Purified Gambir (Uncaria gambir Roxb.) to Liver Histology and its Reversibility on Rats

Permatasari

Oktavia

Nazar

et al. 2021

BSc

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Purified gambir proved to have many pharmacological activities and has the potential to be developed into standardized herbal medicines. This study was conducted to determine the safety of purified gambir to liver histology. A total of 30 female white rats were used which divided into four groups: one control group and three test groups treated with purified gambir at doses of 5, 10, and 20 mg/kgbw orally for 14 days, respectively. On the scheduled days, three animals from each group were killed for liver histological examination. The degree of liver damage and liver organ ratio in this study where were measured. The reversibility of liver damage was observed from the change in the value of the damage degree, and liver organ ratio after the administration of gambir was stopped. Data were analyzed using twoway ANOVA followed by Duncan (significance was taken at p<0.05). The administration of purified gambir in rats for 14 days at tested groups caused significant liver tissue damage (p<0.05). In this situation, the degree of tissue damage increased, and the liver organ ratio decreased in all tested groups. After the administration was stopped, the liver damage degree decreased insignificantly, while the liver organ ratio is significantly increased.

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The Impact of Sub Acute Administration of Purified Gambier (Uncaria gambir Roxb.) to The Liver and Kidney Functions and its Reversibility on Rats

Cited by 6 publications

References 4 publications

Comparison of Gambier Extract (Uncaria Gambier Robx) and Angiotensin Receptor Blocker on Proteinuria Reduction and Antioxidants - Enhancement in Nephrotic Rat Models

Comparison of Gambier Extract (Uncaria Gambier Robx) and Angiotensin Receptor Blocker on Proteinuria Reduction and Antioxidants - Enhancement in Nephrotic Rat Models

Purified Gambir (Uncaria Gambir (Hunter) Roxb.) Attenuate Renal Function in Alloxan-Induced Diabetic Male Rats

The Sub Acute Toxicity Study of Purified Gambir (Uncaria gambir Roxb.) to Liver Histology and its Reversibility on Rats

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