The impact of onion-garlic mixture to control of Rhynchophorus ferrugineus in Saudi Arabia

Al-Shuraym, Laila A.; Al-Keridis, Lamya Ahmed; Al-Dakhil, Abeer A.; Al-Qahtani, Wedad Saeed

doi:10.1016/j.jssas.2020.09.005

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…al., 2016;Rinaldi, et. al., 2019;Al-Shuraym, et. al., 2020), aroma sulfur yang berasal dari bawang putih memberi efek repellent terhadap serangga vektor.…”

Section: Gambar 5 Pendampingan Pengaplikasian Pestisida Nabatiunclassified

Pelatihan Pembuatan dan Pendampingan Aplikasi Pestisida Nabati Pada Kelompok Petani Cabai Rawit di Desa Tumbubara Kec. bajo Barat

Zakiyah

Yanti

2022

JAI

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Meningkatnya curah hujan, mendorong peningkatan hama pada tanaman cabai rawit antara lain hama semut dan hama aphids. Petani cabai rawit di Desa Tumbubara, Kec. Bajo Barat, Kab. Luwu cenderung membiarkan hama bertambah banyak, menyebabkan hamper seluruh daun tanaman dalam satu lahan cabai rawit terserang hama. Kegiatan ini dilaksanakan dengan tujuan memperkenalkan pestisida nabati yang mampu mengurangi jumlah hama pada tanaman cabai rawit dalam waktu relative cepat. Berdasarkan monitoring dan evaluasi yang dilakukan pada efektivitas pestisida nabati terhadap pengurangan hama tanaman cabai rawit, diketahui bahwa dalam kurun waktu 7 hari setelah aplikasi, jumlah hama mulai berkurang dan tanaman cabai rawit mulai menumbuhkan pucuk daun muda yang lebih sehat. Kegiatan ini dapat berhasil berkat keikutsertaan petani yang mengaplikasikan pestisida nabati pada tanaman cabai rawit secara berkala terutama setelah hujan.

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“…al., 2016;Rinaldi, et. al., 2019;Al-Shuraym, et. al., 2020), aroma sulfur yang berasal dari bawang putih memberi efek repellent terhadap serangga vektor.…”

Section: Gambar 5 Pendampingan Pengaplikasian Pestisida Nabatiunclassified

Pelatihan Pembuatan dan Pendampingan Aplikasi Pestisida Nabati Pada Kelompok Petani Cabai Rawit di Desa Tumbubara Kec. bajo Barat

Zakiyah

Yanti

2022

JAI

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“…Moreover, toxic levels against red palm weevil larvae and pupae are quite high when onion and garlic are combined. Esterase, the expression of glutathione S transferase (GST), and cytochrome P450 genes are all shown to be preferentially suppressed, indicating the mixture’s bioactive activity [ 20 ]. O. basilicum ’s cell suspension extract contains volatile and non-volatile secondary metabolites that demonstrate insecticidal activity against R. ferruginous ’s adults and larvae [ 11 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring Ocimum basilicum’s Secondary Metabolites: Inhibition and Molecular Docking against Rhynchophorus ferrugineus for Optimal Action

Darrag,

Ghazzawy,

Nasser Alzain

et al. 2024

Plants

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The objective of our work is to create a practical procedure to produce in vitro cell suspensions of O. basilicum and to ascertain the factors that encourage enhanced secondary metabolite production. We investigated the impact of these metabolites on Rhynchophorus ferrugineus’s adult and larval target enzymes. The explants were cultivated on Murashige and Skoog (MS) media with 0.1 to 1 mg/L plant growth regulators (PGRs) to create calluses. 2,4-Dichlorophenoxyacetic acid (2,4-D), kinetin, 1-naphthylacetic acid (NAA), and indole-3-butryic acid (IBA) at 0.5, 0.5, 0.1, and 1 mg/L, respectively, with 3% sucrose led to the highest biomass accumulation. In cell suspensions, the total phenolic content (TPC) and total flavonoid content (TFC) were 39.68 and 5.49 mg/g DW, respectively, with abiotic Verticillium dahliae as an activator. Rosmarinic acid, ursolic acid, nepetoidin A and B, salvigenin, and quercetin-3-O-rutinoside as flavonoids and phenolics were analyzed using UPLC-I TQD MS, with the highest concentrations reached after 40 days. The extract demonstrates insecticidal activity against the fourth-instar larvae of R. ferrugineus, with adults at 1197 µg/mL and 12.5 µg/larvae as LC50 and LD50 values. The extract inhibited acetylcholine esterase (AChE), acid phosphatases (ACPs), alkaline phosphatases (ALPs), and gamma-aminobutyric acid-transaminase (GABA-T) in larval tissue in vitro, with IC50 values of 124.2, 149.3, 157.8, and 204.8 µg/mL, and in vivo, with IC50 values of 157.2, 179.4, 185.3, and 241.6 µg/mL, after 24 h. Pure compounds identified the activity of the extract, showing the inhibition of AChE, ACPs, ALPs, and GABA-T with IC50 values ˂ 200 µg/mL (in vitro). The ABMET examination revealed good oral permeability, and docking tests showed that the compounds bind AChE, ACPs, ALPs, and GABA-T. These findings show that a green bioprocessing method such as an O. basilicum cell suspension is a quick and straightforward technique for producing phenolic compounds, and it may be used to develop sustainable bio-insecticides and new green procedures.

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The impact of the onion-garlic extracts to control date palm aphids in Saudi Arabia

Al-Shuraym

2022

Journal of the Saudi Society of Agricultural Sciences

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The impact of onion-garlic mixture to control of Rhynchophorus ferrugineus in Saudi Arabia

Cited by 5 publications

References 17 publications

Pelatihan Pembuatan dan Pendampingan Aplikasi Pestisida Nabati Pada Kelompok Petani Cabai Rawit di Desa Tumbubara Kec. bajo Barat

Pelatihan Pembuatan dan Pendampingan Aplikasi Pestisida Nabati Pada Kelompok Petani Cabai Rawit di Desa Tumbubara Kec. bajo Barat

Exploring Ocimum basilicum’s Secondary Metabolites: Inhibition and Molecular Docking against Rhynchophorus ferrugineus for Optimal Action

The impact of the onion-garlic extracts to control date palm aphids in Saudi Arabia

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