2017
DOI: 10.1111/jfpe.12649
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The impact of membrane pretreatment on the enzymatic production of whey‐derived galacto‐oligosaccharides

Abstract: This study presents a multistep process for the valorization of whey that involves membrane filtration techniques to fractionate whey compounds and the subsequent enzymatic conversion of lactose into galacto‐oligosaccharides with a degree of polymerization (DP) of 3–5. Partially demineralized whey was first concentrated and diafiltered by ultrafiltration (UF) to obtain whey protein isolates. Over 97% of the initial amount of lactose was recovered into the permeate which was then concentrated by nanofiltration … Show more

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Cited by 11 publications
(6 citation statements)
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References 36 publications
(58 reference statements)
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“…Carbohydrate composition (glucose, galactose, and DP2-6 fractions) of samples was determined by the HPLC method, as previously described in [32]. In brief, a Thermo Separations HPLC system equipped with a Shodex R-101 refractive index detector (Showa Denko Europe GmbH, Munich, Germany) and a N2000 Chromatography Data System from Science Technology (Hangzhou) Inc. (Hangzhou, China) was used, employing an RNM carbohydrate 8% Na + 300 × 7.8 (Phenomenex, Torrance, CA, USA) analytical column at 50 • C at 0.2 mL•min −1 with a prefiltered (2 µm) DI water as mobile phase.…”
Section: High Performance Liquid Chromatographymentioning
confidence: 99%
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“…Carbohydrate composition (glucose, galactose, and DP2-6 fractions) of samples was determined by the HPLC method, as previously described in [32]. In brief, a Thermo Separations HPLC system equipped with a Shodex R-101 refractive index detector (Showa Denko Europe GmbH, Munich, Germany) and a N2000 Chromatography Data System from Science Technology (Hangzhou) Inc. (Hangzhou, China) was used, employing an RNM carbohydrate 8% Na + 300 × 7.8 (Phenomenex, Torrance, CA, USA) analytical column at 50 • C at 0.2 mL•min −1 with a prefiltered (2 µm) DI water as mobile phase.…”
Section: High Performance Liquid Chromatographymentioning
confidence: 99%
“…In brief, a Thermo Separations HPLC system equipped with a Shodex R-101 refractive index detector (Showa Denko Europe GmbH, Munich, Germany) and a N2000 Chromatography Data System from Science Technology (Hangzhou) Inc. (Hangzhou, China) was used, employing an RNM carbohydrate 8% Na + 300 × 7.8 (Phenomenex, Torrance, CA, USA) analytical column at 50 • C at 0.2 mL•min −1 with a prefiltered (2 µm) DI water as mobile phase. Specification of standards used for peak identification can be found in [32]. Samples taken from the product (= permeate) stream of the EMR were not subject to heat deactivation prior to HPLC analysis unless otherwise stated.…”
Section: High Performance Liquid Chromatographymentioning
confidence: 99%
See 1 more Smart Citation
“…Data collection and peak integration was performed with N2000 Chromatography Data System and N2000 Photographic Data Workstation software package (Science Technology Inc., Hangzhou, China). Peak identification and determination of the concentration of individual carbohydrate fractions was performed as described earlier in (Pázmándi, Maráz, Ladányi, & Kovács, 2018). Analytic grade ethanol was used as a standard to detect and quantify ethanol production.…”
Section: Methodsmentioning
confidence: 99%
“…A 20-or 100-ml GOS fermentation media in 100-or 500-ml Erlenmeyer flasks, respectively, were inoculated with 5 × 10 6 cells per ml and incubated by shaking with 220 rpm at 30 C. (Pázmándi, Maráz, Ladányi, & Kovács, 2018). Analytic grade ethanol was used as a standard to detect and quantify ethanol production.…”
Section: Laboratory Fermentationsmentioning
confidence: 99%