Continuous Production of Galacto-Oligosaccharides by an Enzyme Membrane Reactor Utilizing Free Enzymes

Cao, Teng; Pázmándi, Melinda; Galambos, Ildikó; Kovács, Zoltán

doi:10.3390/membranes10090203

Cited by 15 publications

(30 citation statements)

References 34 publications

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“…In EMRs, enzymes are retained in the reactor by membranes with proper molecular weight cut-off (MWCO). EMRs are scalable and have already been applied to a large variety of products [ 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Production of Modified Nucleosides in a Continuous Enzyme Membrane Reactor

Thiele

Yehia

Krausch

et al. 2023

IJMS

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Nucleoside analogues are important compounds for the treatment of viral infections or cancers. While (chemo-)enzymatic synthesis is a valuable alternative to traditional chemical methods, the feasibility of such processes is lowered by the high production cost of the biocatalyst. As continuous enzyme membrane reactors (EMR) allow the use of biocatalysts until their full inactivation, they offer a valuable alternative to batch enzymatic reactions with freely dissolved enzymes. In EMRs, the enzymes are retained in the reactor by a suitable membrane. Immobilization on carrier materials, and the associated losses in enzyme activity, can thus be avoided. Therefore, we validated the applicability of EMRs for the synthesis of natural and dihalogenated nucleosides, using one-pot transglycosylation reactions. Over a period of 55 days, 2′-deoxyadenosine was produced continuously, with a product yield >90%. The dihalogenated nucleoside analogues 2,6-dichloropurine-2′-deoxyribonucleoside and 6-chloro-2-fluoro-2′-deoxyribonucleoside were also produced, with high conversion, but for shorter operation times, of 14 and 5.5 days, respectively. The EMR performed with specific productivities comparable to batch reactions. However, in the EMR, 220, 40, and 9 times more product per enzymatic unit was produced, for 2′-deoxyadenosine, 2,6-dichloropurine-2′-deoxyribonucleoside, and 6-chloro-2-fluoro-2′-deoxyribonucleoside, respectively. The application of the EMR using freely dissolved enzymes, facilitates a continuous process with integrated biocatalyst separation, which reduces the overall cost of the biocatalyst and enhances the downstream processing of nucleoside production.

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Section: Introductionmentioning

confidence: 99%

Production of Modified Nucleosides in a Continuous Enzyme Membrane Reactor

Thiele

Yehia

Krausch

et al. 2023

IJMS

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“…Co ´rdova et al [21] (22 h), Ren et al [19] (96 h) and Cao et al [18] (120 h) focused on the long-term performance. They demonstrated that by adding periodically enzyme [18,19] a stable GOS production over an extended time period can be realized. However, this was accompanied by an increase in TMP [18] or pronounced flux decline [21].…”

Section: Introductionmentioning

confidence: 99%

“…They demonstrated that by adding periodically enzyme [18,19] a stable GOS production over an extended time period can be realized. However, this was accompanied by an increase in TMP [18] or pronounced flux decline [21].…”

Section: Introductionmentioning

confidence: 99%

Potential of Integrated Semi‐Continuous Enzymatic Synthesis and Filtration Processes for Efficiency Enhancement

Hofmann

Hamel

2023

Chemie Ingenieur Technik

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Galacto‐oligosaccharides (GOS), increasingly popular prebiotics, are synthesized by enzymatic conversion of lactose. Among others, the total production costs are significantly influenced by the costly enzyme. Therefore, it was investigated if the reuse and full recovery of the enzyme is feasible, followed by the development of a semi‐continuous process in order to maintain a consistent high GOS yield. As a preliminary step, the enzyme‐catalyzed reaction was recorded within the permissible operating parameters. It was successfully shown that steady high GOS yields can be synthesized semi‐continuously within a filtration plant functioning as an enzymatic membrane reactor.

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“…GOS are safest oligosaccharides, a functional food additive with natural properties [24]. It is an important prebiotic for obtaining wide recognition and optimal therapeutic effect [25]. It has been widely used in infant formula, fermented milk and other foods [26].…”

Section: Introductionmentioning

confidence: 99%