The Impact of IFN‐γ Gene Polymorphisms on Spontaneous Clearance of HCV Infection in Fars Province, Southern of Iran

Sarvari, Jamal; Moattari, Afagh; Pirbonyeh, Neda; Moini, Maryam; Hosseini, Seyed Younes

doi:10.1002/jcla.21855

Cited by 13 publications

(15 citation statements)

References 47 publications

(68 reference statements)

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“…28 A study conducted on patients from Southern Iran with genotypes 1, 2, and 3 demonstrated no association between IFNγ +874 polymorphisms and HCV infection outcomes. However, clearance was associated only with haplotype (A allele at +874 loci and G allele at +2109 loci), 29 whereas liver cirrhosis was reported to be associated with the T allele at position +874 of the IFN-γ gene in HCV-infected Taiwanese patients. 30 In contrast to our results, a study conducted on Caucasian patients who underwent liver transplantation for end-stage liver disease due to HCV infection revealed that the IFN-γ +874 polymorphism genotypes are not related to liver fibrosis progression in recurrent hepatitis C patients.…”

Section: Discussionmentioning

confidence: 98%

“…A study conducted on patients from Southern Iran with genotypes 1, 2, and 3 demonstrated no association between IFN‐γ +874 polymorphisms and HCV infection outcomes. However, clearance was associated only with haplotype (A allele at +874 loci and G allele at +2109 loci), whereas liver cirrhosis was reported to be associated with the T allele at position +874 of the IFN‐γ gene in HCV‐infected Taiwanese patients …”

Section: Discussionmentioning

confidence: 99%

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Association of interferon gamma gene polymorphism and susceptibility to hepatitis C virus infection in Egyptian patients: A multicenter, family‐based study

et al. 2017

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Background and AimPolymorphisms in some genes may influence the persistence of hepatitis C virus (HCV) infection, clinical outcome, HCV replication, and liver damage. This study was conducted to investigate the role of the interferon gamma (IFN‐γ) gene at (+874 T/A, −764 G/C, −179 C/A) single‐nucleotide polymorphisms (SNPs) and its receptor (IFN‐γR2) at (rs 2786067 A/C) SNP in the susceptibility of Egyptian families to HCV infection with high‐resolution techniques.MethodsIn total, 517 Egyptian families, with 2246 subjects, were recruited to this study from the Upper and Lower Egypt governorates and were classified into three groups: 1034 patients with chronic hepatitis C virus, 108 subjects with spontaneous virus clearance (SVC), and 1104 subjects as a healthy control group. All subjects were genotyped for (+874 T/A, rs2430561, −764 G/C, rs2069707, −179 C/A, rs2069709, and rs 27860067, A/C) SNPs of the IFN‐γ gene using the allelic discrimination real‐time polymerase chain reaction technique and were confirmed using sequence‐based typing.ResultsThe carriage of T allele of (+874) IFN‐γ is a risky allele and was significantly higher in chronic hepatitis C more than other two groups (odds ratio [OR]: 2.6646, P < 0.0002). On the other hand, the C allele of (−764, rs2069707) is a protective allele and was higher in SVC than the other two groups (OR: 0.2709, P < 0.0001). However, both (−179 C/A, rs 2069709) and (rs 27860067, A/C) SNPs are not polymorphic enough to be studied in the Egyptian population.ConclusionsHCV infection is associated with the T allele of (+874 rs2430561), while SVC of HCV is associated with the C allele of (−764, rs2069707) of the IFN‐γ gene.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Association of interferon gamma gene polymorphism and susceptibility to hepatitis C virus infection in Egyptian patients: A multicenter, family‐based study

et al. 2017

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“…The status of HCV infection (Chronic or spontaneously clearance) was determined through patients' medical records, but further confirmed by serological and molecular methods as described previously (Sarvari et al, 2016[ 22 ]). Briefly, all sera samples were screened by ELISA assay to confirm serological state.…”

Section: Methodsmentioning

confidence: 99%

Association of PD-1 gene with outcome of hepatitis C virus infection

Sarvari

Dowran

Hosseini

et al. 2018

EXCLI Journal; 17:Doc935; ISSN 1611-2156

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“…Chronic or resolved HCV infection was diagnosed through medical records and was then confirmed by performing ELISA and RT-PCR as described before (18,19). Briefly, all samples were screened by ELISA assay to confirm serologic state.…”

Section: Viral Infection Statementioning

confidence: 99%

“…The presence of virus genome was also evaluated by a gel-based and real-time PCR method. Briefly, a verified in-house Nested-PCR method, which was developed in our lab, was applied as a screening method (18). In addition, a commercial qualitative real-time PCR for virus detection (Amplisens HCV-FRT, Russia) was also employed.…”

Section: Viral Infection Statementioning

confidence: 99%

Association of Genotype and Haplotype of IL-28B Gene with Hepatitis C Infection Outcome in Iran: Spontaneous Clearance Versus Chronic Infection

et al. 2017

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Background: Spontaneous viral clearance occurs in 10% to 40% of individuals after hepatitis C virus infection. Some polymorphisms in IL-28B gene may influence the outcome of HCV infection. The present study aimed at investigating the genotype and allele frequency of IL-28B rs12979860 and rs8099917 SNPs in HCV infected patients in addition to determining their association with disease outcome. Methods: A total of 302 patients with chronic hepatic C infection and 36 individuals whose infection was spontaneously cleared were included in this case-control study. The presences of chronic or spontaneously cleared infection in participants were determined by serologic and molecular methods. Genomic DNA of the participants was extracted using salting out method. IL-28B/IFN-λ3 gene polymorphisms were conducted using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The frequency of CC genotype (P = 0.001) and C allele (P = 0.0007) of IL-28B gene at rs12979860 SNP was significantly higher in participants with spontaneously cleared HCV infection compared to that of those who were chronically infected. In the case of rs8099917 SNP of IL-28B, no correlation was found between frequency of genotype (P = 0.17) or allele (P = 0.12) and HCV infection outcome. The results of haplotype analysis showed the association of CT (P = 0.012) and TT (P = 0.013) haplotypes with spontaneous clearance and chronic infection, respectively. Conclusions: The findings implied that individuals with CC or CT genotype at rs12979860 SNP but rs8099917 SNP was not in association with spontaneous clearance of HCV in an Iranian population with HCV infection.

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The Impact of IFN‐γ Gene Polymorphisms on Spontaneous Clearance of HCV Infection in Fars Province, Southern of Iran

Abstract: Our finding indicated that individuals with GG genotype at +2109 loci of IFN-γ gene and also AG haplotype (A allele at +874 loci and G allele at +2109 loci) may clear HCV infection more frequently than those with AA and AG genotype at +2109 loci and AA, TA, and TG haplotype.

Cited by 13 publications

References 47 publications

Association of interferon gamma gene polymorphism and susceptibility to hepatitis C virus infection in Egyptian patients: A multicenter, family‐based study

Association of interferon gamma gene polymorphism and susceptibility to hepatitis C virus infection in Egyptian patients: A multicenter, family‐based study

Association of PD-1 gene with outcome of hepatitis C virus infection

Association of Genotype and Haplotype of IL-28B Gene with Hepatitis C Infection Outcome in Iran: Spontaneous Clearance Versus Chronic Infection

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