The study "Traditional Observance and Cultural Significance of the June 6 th Sacrifice Festival" delves into the unique and time-honored celebration observed by the Miao ethnic group in the regions of Renhuai and Tongzi Town, located in Guizhou province, China. This paper presents a comprehensive case study that explores the festival's historical roots, its traditional observance, and the cultural significance held for the Miao community in these specific locations. Ethnographic research techniques, including participant observation, interviews with local community members, and examination of Literature, are utilized to gain an indepth understanding of the festival's practices and its role in the Miao culture. The findings reveal that the June 6 th Sacrifice Festival is central to the Miao people's social and religious life. It serves as a significant occasion for expressing reverence to their ancestors and deities, particularly the revered God Chiyou ( 蚩尤 ), who is regarded as a symbol of strength and bravery. The festival is characterized by elaborate rituals, vibrant performances of traditional dances, and the preparation of symbolic offerings to honor the spirits of their ancestors. Moreover, this study highlights the festival's impact on community cohesion and cultural identity, fostering a strong sense of belonging and pride among the Miao people in Renhuai and Tongzi Town. It functions as a vital platform for transmitting cultural heritage from one generation to another, reinforcing their distinct ethnic identity and promoting intergenerational continuity of traditions. It contributes to the existing literature on ethnic festivals and cultural preservation, offering valuable insights for scholars, policymakers, and cultural enthusiasts alike, while also recognizing the importance of safeguarding and promoting intangible cultural heritage in the region.