The Impact of Aspirin Intake on Lactate Dehydrogenase, Arterial Stiffness, and Oxidative Stress During High‐Intensity Exercise: A Pilot Study

Abstract

Aspirin is a common nonsteroidal anti-inflammatory drug used to reduce fever, pain, and inflammation. However, aspirin’s anti-inflammatory properties may also prevent increased levels of blood lactate dehydrogenase, vascular arterial stiffness and oxidative stress induced by high-intensity exercise. The purpose of this study was to investigate the effects of 4 weeks of aspirin supplementation on lactate dehydrogenase activity, lactate, arterial stiffness, and antioxidant capacity during high-intensity exercise in Taekwondo athletes. Participants were randomly divided into two groups: aspirin supplementation (n = 10) and placebo-control (n = 10). Blood levels of lactate dehydrogenase (LDH) enzyme activity and lactate were assessed to examine muscle damage and carotid-to-radial pulse wave velocity and the augmentation index were measured to examine arterial stiffness. Blood levels of superoxide dismutase, malondialdehyde, and glutathione peroxidase were assessed to determine antioxidant capacity and levels of oxidative stress. There were significant group × time interactions for enzyme activity of LDH (Δ-60 ± 24.36 U/L) and carotid-to-radial pulse wave velocity (Δ-1.33 ± 0.54 m/s), which significantly decreased (p < 0.05) following aspirin supplementation compared to placebo-control. Superoxide dismutase (Δ359 ± 110 U/gHb) and glutathione peroxidase (Δ28.2 ± 10.1 U/gHb) significantly decreased while malondialdehyde (0Δ3.0 ± 0.1 mmol/mL) significantly increased (p < 0.05) in the placebo-control group compared to the supplementation group. However, there were no changes in lactate concentration levels or augmentation index. These results reveal that low-dose aspirin supplementation would be a useful supplementation therapy to prevent high-intensity exercise training-induced increases in oxidative damage, inflammation, skeletal muscle fatigue, and arterial stiffness in elite Taekwondo athletes.

Acute mitochondrial antioxidant intake improves endothelial function, antioxidant enzyme activity, and exercise tolerance in patients with peripheral artery disease

“…The absorbances of the incubated samples were assessed at 450 nm with a microplate reader. SOD activity was measured by a Cobas Mira chemistry analyzer (Roche, Basel, Switzerland) (25). MDA was assessed by a commercially available Lipid Peroxidation (MDA) Assay Kit (Sigma Aldrich, St. Louis, MO).…”

“…Samples were incubated with a reagent diluent for 5 min at 23°C. The incubated samples were stimulated by Ran-Cell total antioxidant control (Randox, Crumlin, County Antrim, UK) and were analyzed at 340 nm by a microplate reader (25). The mean intra-assay variability measured as the coefficient of variation for each sample was 2.9, 3.8, and 3.3% for SOD, TAC, and MDA, respectively.…”

Effects of l-citrulline supplementation on nitric oxide and antioxidant markers after high-intensity interval exercise in young men: a randomised controlled trial

“…In line with our results, da Silva et al(2014) (29) indicated that taurine supplementation increases SOD, GPx, and CAT concentrations following eccentric exercise (29) . In contrast, Lee et al (2020) (30) showed no changes in SOD and GPx concentrations following four weeks of high-intensity exercise combined with aspirin consumption. It seems that disparities in exercise and trial duration (acute vs longitudinal),as well as supplementation type, are probable reasons for these inconsistencies (30) .…”

“…In contrast, Lee et al (2020) (30) showed no changes in SOD and GPx concentrations following four weeks of high-intensity exercise combined with aspirin consumption. It seems that disparities in exercise and trial duration (acute vs longitudinal),as well as supplementation type, are probable reasons for these inconsistencies (30) . Antioxidant defensive systems are in control of scavenging ROS in Accepted manuscript order to avoid oxidative stress.…”

"Lactate Dehydrogenase a Potential Diagnostic Biomarker for Cancer: A Review of Literature"

“…In [33]. In the case of ovarian cancer, serum LDH may be a useful diagnostic for distinguishing between ovarian carcinoma and benign ovarian tumours (Table 2) [34][35][36][37][38][39][40][41][42][43][44][45][46].…”