The impact of allicin on lead-induced oxidative damage in selected organs of the common carp (Cyprinus carpio)

Shahsavani

2019

IJT

Self Cite

Background: This study was conducted to investigate whether vitamin C can protect against plasma biochemical changes induced by lead poisoning in Cyprinus carpio. Methods: Three groups of common carp (n=30/group) were used in this study. Group 1 served as control, Group 2 was exposed to lead acetate (5 mg/L) for 15 days, and Group 3 received vitamin C (500 mg/kg) during the same duration of lead exposure. Results: The blood lead concentrations in Groups 2 and 3 showed a significant rise as compared to that in the control group (p<0.05). Also, the plasma malondialdehyde (MDA) concentration increased considerably following in groups exposed to lead acetate, compared to that in controls. Vitamin C supplementation decreased the rise in plasma MDA insignificantly, compared that in Group 2. The plasma values of creatinine and alanine aminotransferase (ALT) increased in Group 2 compared to that in controls. Also, vitamin C treatment significantly decreased plasma ALT and creatinine concentrations, compared to those in Group 2. Values of other plasma parameters including aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, gammaglutamyl transferase, albumin, glucose, total protein, cholesterol, urea, uric acid and triglyceride showed no significant alterations among the treatment groups. Conclusion: The results suggest that vitamin C have some beneficial effects against lead toxicity in common carp. However, elucidation of the precise mechanism of the protective effects of vitamin C against lead toxicity warrants further investigations

Section: Discussionsupporting

confidence: 92%

“…Considering the rich contents of polyunsaturated fatty acids in fish, they are very sensitive to lipid peroxidation, caused by exposure to toxic heavy metal (18). The significant rise in the plasma MDA concentration of lead-exposed C. carpio, as found in this study, is consistent with that reported in other species (19)(20)(21)(22).…”

Section: Discussionsupporting

confidence: 91%

The Effect of Vitamin C on Lead-induced Plasma BiochemicalAlterations in Fish, Cyprinus carpio

Nourian

Shahsavani

2019

IJT

Self Cite

“…It has been also reported that both vitamins C and E are effective in decreasing lead-induced elevated values of TBARS by 60 and 30 percent, respec-tively [24]. Allicin was markedly effective in decreasing lead-induced lipid peroxidation and increasing the cellular antioxidant enzyme activities and GSH levels [31]. Despite known property of CA as an antioxidant [7,8], its administration in the present work had no significant effect on increased values of MDA in erythrocyte haemolysate of mice in group 3 as compared to lead-exposed animals in group 2.…”

Section: Discussionmentioning

confidence: 85%

Ameliorative Effects of Caffeic Acid on Lead Accumulation and Oxidative Stress in Lead-Exposed Mice

Lotfi-Ghahramanloo

2016

Zahedan J Res Med Sci

Self Cite

“…GPx is an important antioxidant enzyme protecting tissues from oxidative damage by reducing hydrogen peroxide and a wide range of organic hydroperoxides. GSH also plays an import function in detoxification reactions by acting both as a nucleophilic scavenger of various undesired compounds and their toxic metabolites and as a specific substrate for some endogenous detoxification pathways (Drag‐Kozak et al, 2018; Kheradmand et al, 2013; Shahsavani et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Determination of plasma malondialdehyde (MDA) concentration was based on spectrophotometric analysis of the pink coloured product of thiobarbituric acid‐reactive substances (TBARS; Shahsavani et al, 2012). The concentration of MDA was calculated using a molar extinction coefficient value of 156,000 M −1 cm −1 and the results were expressed as nanomoles of MDA per ml of plasma.…”

Section: Methodsmentioning

confidence: 99%

Impact of betaine and allicin against circulating oxidative and biochemical alterations induced by cadmium intoxication in common carp

Shahsavani

Nourian

2022

Aquaculture Research

Self Cite

This work aimed to evaluate the antidotal role of allicin and/or betaine versus experimental Cd toxicity in terms of restoring Cd‐provoked changes in the circulating oxidative biomarkers together with biochemical indices of tissue abnormalities in common carp. 150 healthy common carp were subdivided into five equal groups. Group 1 fish (control) was reared in normal freshwater. Fish in other groups were exposed to 5 mg/L cadmium chloride in water for 15 days. Group 3 fish received diet supplemented with betaine (30 mg/kg BW); Group 4 fish received diet supplemented with allicin (15 mg/kg BW) and in group 5, fish food was supplemented with both betaine and allicin. Cd exposure caused significant decline in circulating SOD, catalase, GSH and FRAP as well as a rise in MDA values. Plasma levels of ALT, GGT, ALP, glucose, urea and creatinine also showed significant increase in fish treated with cadmium compared with controls. However, allicin and/or betaine supplementation had been somewhat fortunate in recovering Cd‐provoked shifts in the circulating oxidative biomarkers and biochemical indicators of tissue abnormalities in common carp which may be relevant to antioxidant properties of these compounds as well as metal‐chelating and methyl‐donating abilities of allicin and betaine respectively. Moreover, combination of allicin and betaine stands outs as the most effective approach upon Cd poisoning. However, explanation of the precise molecular mechanisms of their modulating effects against Cd poisoning as well as their optimal dosage in various exposure durations and toxicant concentrations requires further study.