The Effect of Vitamin C on Lead-induced Plasma BiochemicalAlterations in Fish, Cyprinus carpio

Abstract

Background: This study was conducted to investigate whether vitamin C can protect against plasma biochemical changes induced by lead poisoning in Cyprinus carpio. Methods: Three groups of common carp (n=30/group) were used in this study. Group 1 served as control, Group 2 was exposed to lead acetate (5 mg/L) for 15 days, and Group 3 received vitamin C (500 mg/kg) during the same duration of lead exposure. Results: The blood lead concentrations in Groups 2 and 3 showed a significant rise as compared to that in the control group (p<0.05). Also, the plasma malondialdehyde (MDA) concentration increased considerably following in groups exposed to lead acetate, compared to that in controls. Vitamin C supplementation decreased the rise in plasma MDA insignificantly, compared that in Group 2. The plasma values of creatinine and alanine aminotransferase (ALT) increased in Group 2 compared to that in controls. Also, vitamin C treatment significantly decreased plasma ALT and creatinine concentrations, compared to those in Group 2. Values of other plasma parameters including aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, gammaglutamyl transferase, albumin, glucose, total protein, cholesterol, urea, uric acid and triglyceride showed no significant alterations among the treatment groups. Conclusion: The results suggest that vitamin C have some beneficial effects against lead toxicity in common carp. However, elucidation of the precise mechanism of the protective effects of vitamin C against lead toxicity warrants further investigations

An evaluation of the effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-intoxicated rabbits

“…In Group 2, ( N = 10) the rabbits received lead acetate 547 ppm (Merck Co, Germany) orally in a dose of 5 mg/L by stomach tube once daily (5 mg Pb dissolved in 1 L water). 12 , 17 …”

“…A 500 mg/kg vitamin C was dissolved in 100 cc water and any rabbit was gavage daily 10 mL distilled water. 12 At the end of the experiment, blood samples were drawn and the level of blood lead was calculated using blood lead analyzer (Magellan Diagnostics, USA). Plasma level of lead was calculated in µg/dL.…”

“…However, 10 cc of the blood samples was drawn and dumped in heparin-sealed plastic tubes and sent immediately to the laboratory to be measured using Method 8003 American National Institute of Occupational Safety and Health (NIOSH). 12 …”

“…This vitamin is a Lactone with an Enediol group that can be combined with lead to form a very low ionic but water-soluble compound. 12,13…”

PENINGKATAN LAJU PERTUMBUHAN SPESIFIK IKAN LELE (Clarias sp) DENGAN SUPLEMENTASI VITAMIN C PADA PAKAN

“…Tingkat efisiensi pakan lebih baik dibandingkan dengan kontrol memiliki arti bahwa vitamin C yang diberikan memberikan pengaruh mesikupun tidak nyata secara statistik sig>0.05. Beberapa manfaat vitamin C yang didapatkan dengan cara pemberian secara rutin diantaranya meningkatkan kadar plasma darah menurunkan konsentrasi stress dalam darah dapat meningkatkan ketahanan tubuh dari serangan patogen (Nourian et al, 2021). Narra et al, (2015) menyatakan bahwa secara nyata suplementasi vitamin C dapat meningkatkan pertumbuhan, kelulushidupan, kondisi biokimia dan profil darah pada ikan.…”