The immunoperoxidase localization of tumour markers in ovarian cancer: the value of CEA, EMA, cytokeratin and DD9

Hammond, R. H.; Bates, Thelma D.; Clarke, D. G.; Grant, Anne G.; Haines, A. M. R.; Eustace, D.; Heyderman, Eadie

doi:10.1111/j.1471-0528.1991.tb10315.x

Cited by 15 publications

(10 citation statements)

References 42 publications

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“…As regards undifferentiated carcinomas the results of the present study were consistent with the findings of Hammond et al (22) , (using monoclonal antiserum). and Fleuren and Nap ( 26 ) , (using polyclonal antiserum).…”

Section: Discussion Histological Type Of Ovarian Tumorssupporting

confidence: 93%

“…While Neunteufel and Breitenecker (19) and Dabbs and Geisinger ( 25 ) , reported 21% and 52% as incidences of positivity respectively. On the other hand, charpin et al (10) , Motoyama et al (13) and Hummond et al (22) , using monoclonal antiserum in their techniques found positive cases of serous ovarian carcinomas in 0%, 0% and 24% of their studied groups respectively.…”

Section: Discussion Histological Type Of Ovarian Tumorsmentioning

confidence: 92%

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A Monoclonal Antibody Quantitative Measurement And Immunohistochemical Localisation Of Carcinoembryonic Antigen In Ovarian Neoplasia

2002

IJGO

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Objective: To evaluate the significance of including CEA in serum and tissues in the management protocol of patients with ovarian malignancies. Patients And Methods: The study included 68 patients which was divided into three groups. Group [A] included 21 patients with malignant ovarian tumors. Group included three patients with boderline ovarian tumors. Group [C] included eight patients with benign ovarian tumors, Group [D] included 36 women without any apparent gynaecologic disorder (control group). Serum level of CEA was measured in all patients in group A, B and C prior to treatment and at least 12 weeks following therapy. Formalin-fixed and paraffin-embedded tissue blocks taken from 2 different sites of the studied lesions were prepared. Immunohistochemical staining for CEA was performed for the studied tissues. Results: All the ovarian tumors investigated in this work were of epithelial origin. All benign and borderline ovarian tumors had negative pre-and post-treatment serum CEA levels (< 5ng/ml) and were negative for CEA tissue stain.85.71% of ovarian serous cystadenocarcinomas had negative pre-treatment serum levels. All the squamous cell carcinomas and 66.67% of each of mucinous cystadenocarcinomas and undifferentiated carcinomas had positive (>=5ng/ml) pre-treatment serum levels. After treatment the different histological types of ovarian carcinimas were seronegative for CEA.The highest percentage of positive staining (100%) was found in squamous cell carcinomas [50% (+1) and 50% (+3)], followed by 66.67% of mucinous cystadenocarcinomasThe mean difference between pre-and posttreatment serum CEA was significant in mucinous cystadinocarcinoma with negative and positive tissue reaction and in negatively stained serous cystadinomacarcinoma.The percentages of cases of ovarian carcinomas with positive pretreatment serum CEA increased with the increase of grade of malignancy from 33.33% in G1 to 55.56% in G II and 66.67% in GIII.No definite relation could be detected between the grade of malignancy and the intensity (degree) of reaction of malignant ovarian tumors to CEA immunostaining.The percentage of cases with positive pre-treatment levels of serum CEA increased progressively with the advance in clinical staging. The incidence was 37.5% in stage I, 55.56% in stage II, 66.67% in stage III, and 100% in stage IV. No definite relation could be detected between the clinical stages and the degree of reaction of ovarian carcinomas to CEA tissue stain. Conclusion: This study indicated that immunohistochemical identification of CEA in tumor tissue and of monoclonal antibodies quantitative measurement of CEA in human serum is a useful adjunct in the management protocol of patients with ovarian malignancies. However further studies are required to fully ascertain the usefullness of this technique.

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Section: Discussion Histological Type Of Ovarian Tumorssupporting

confidence: 93%

Section: Discussion Histological Type Of Ovarian Tumorsmentioning

confidence: 92%

A Monoclonal Antibody Quantitative Measurement And Immunohistochemical Localisation Of Carcinoembryonic Antigen In Ovarian Neoplasia

2002

IJGO

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“…In ovarian carcinomas, CEA is most frequently positive in mucinous carcinomas (70 -80%) and, to a lesser extent (20%), in serous carcinomas. 8 In the four cases of ovarian carcinoma presented, the cells present on the cell block preparation were positive for LMW and HMW cytokeratins and negative for CEA and B72.3, confirming their benign mesothelial nature.…”

Section: Discussionmentioning

confidence: 64%

Diagnostic pitfalls of peritoneal washing cytology and the role of cell blocks in their diagnosis

Selvaggi

2003

Diagnostic Cytopathology

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Mesothelial cell hyperplasia, collagen balls, endometriosis, and endosalpingiosis are diagnostic pitfalls on peritoneal washing cytology in women who present with gynecologic lesions. Over an 8-month period, the peritoneal washings from 10 patients undergoing gynecologic surgery for presumed malignancy showed unusual cytologic findings, several of which posed diagnostic difficulties. The washings from four patients with ovarian carcinomas were cellular and contained clusters and strips of cells with cytologic atypia mimicking malignancy. Confirmation of their benign mesothelial origin was confirmed on immunohistochemistry utilizing cell block preparations. In two cases of endometrial endometrioid carcinoma, the washings contained several clusters of cells surrounding and/or admixed with a globular substance. Due to their similarity to endometrial cells, immunohistochemistry was performed on cell block preparations. The cells were positive for cytokeratin and negative for carcinoembryonic antigen and B72.3, confirming their mesothelial origin. In one case, clinically presumed to be a malignant mass, the washings contained tight clusters of cells with mild cytologic atypia admixed with hemosiderin-laden macrophages. In conjunction with the cell block findings, a diagnosis of endometriosis was made. Extensive endometriosis was found on the surgically resected specimen. In two cases, strips of ciliated epithelial cells resembling tubal epithelium were present on the cytologic and cell block preparations, consistent with endosalpingiosis. The peritoneal washings in one case contained several clusters and balls of atypical cells surrounding microcalcifications on cell block preparation. Since calcification within groups of cells in peritoneal washings always raised the possibility of malignancy, a serous carcinoma of the ovary, particularly of borderline malignancy, would have to be excluded. Fortunately, the resected specimen was free of tumor and showed calcified endosalpingiosis on the ovarian surface. Preparation of cell blocks from peritoneal washings is of value in the work-up and management of patients who present with cytologic mimickers of malignancy on fluid cytology.

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“…Lack of positive staining, however, may also be attributed to the mucin which may exclude the antibodies from free access to cell surface antigens. The use of immunostaining techniques in diagnostic work is a complementary tool to routine histological studies where in many cases difficulty is experienced in distin guishing mucinoid and endometrioid carcinomas from metastatic ovarian involvement [4,6,14,15]. Koelma et al [4] found that using a combination of OV 632 and OC 125, which is an antibody that reacts with CA 125 anti gen, gave a specificity for nonmucinous primary ovarian tumours of 89%, OV 632 being the more specific of the two.…”

Section: Discussionmentioning

confidence: 99%

“…The use of monoclonal antibodies to detect ovarian cancer-associated antigens has been suggested by several investigators [1][2][3][4][5][6].Immunocytochemical methods to dis tinguish the histological type of ovarian tumours would be helpful, especially because the difficulty in differentiation between mucinous and endometrioid ovarian tumours is well known [4,5,7], Furthermore these methods play an important role in the development of therapeutic strate gies for patients [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Immunocytochemical Determination of EGFR, OV 632 and OC 125 in Primary Ovarian Cancer Patients

Athanassiadou

Athanassiades²,

Kyrkou³

et al. 1995

Oncology

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An immunocytochemistry study was performed to compare the immunoreac-tivity of the monoclonal antibodies OC 125, EGFR and OV 632 with smear imprints from 60 ovarian tumours (21 serous cystadenocarcinomas, 12 mucinous cystadenocarcinomas, 9 endometrioid carcinomas, 5 clear cell carcinomas and 5 mixed tumours). Twenty-six patients were premenopausal and 34 postmenopausal. The results showed that 75% of mucinous cystadenocarcinomas were negative for all 3 antigens as were 2 of the 5 (40%) clear cell carcinomas. All other tumours were positive for at least one of the three antigens. OV 632 had an overall sensitivity of 73.3%, EGFR 55% and OC 125 46.6%. Four tumours were OC 125-positive and OV 632-negative. There was no significant difference in positivity of OV 632, OC 125 and EGFR, between pre- and postmenopausal patients ( χ² = 0.03) or between tumours of stage I and stage III [χ² = 0.075 (EGFR) 0.95 (OV 632) and 10.49 (OC 125)]. OV 632 was conducted to be the most sensitive antibody in all types of tumours but OC 125, while less sensitive by itself, increased the overall sensitivity in combination with OV 632 from 73.3 to 80% (not statistically significant).

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The immunoperoxidase localization of tumour markers in ovarian cancer: the value of CEA, EMA, cytokeratin and DD9

Cited by 15 publications

References 42 publications

A Monoclonal Antibody Quantitative Measurement And Immunohistochemical Localisation Of Carcinoembryonic Antigen In Ovarian Neoplasia

A Monoclonal Antibody Quantitative Measurement And Immunohistochemical Localisation Of Carcinoembryonic Antigen In Ovarian Neoplasia

Diagnostic pitfalls of peritoneal washing cytology and the role of cell blocks in their diagnosis

Immunocytochemical Determination of EGFR, OV 632 and OC 125 in Primary Ovarian Cancer Patients

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