The Immunomodulatory Activity of Mori folium, the Leaf of <i>Morus alba</i> L., in RAW 264.7 Macrophages In Vitro

Kwon, Da Hye; Cheon, Ju Young; Choi, Eun-Ho; Jeong, Jin Woo; Lee, Ki Won; Kim, Ki Young; Kim, Sung Goo; Kim, Suhkmann; Hong, Su Hyun; Park, Cheol; Hwang, Hye‐Jin; Choi, Yung Hyun

doi:10.15430/jcp.2016.21.3.144

Cited by 25 publications

(20 citation statements)

References 41 publications

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“…In group III (ALI+NaHS), iNOS expression was significantly increased in comparison with control group but significantly decreased in comparison with ALI group. However, administration of PAG (ALI+PAG) led to significant increase in iNOS expression in comparison with both control and ALI groups (Table 2) is an endotoxin found in the external membrane of gram negative bacteria and causes an intense inflammatory response in mammals (Kwon et al 2016). During the present study, LPS was given for induction of ALI in rats which was confirmed by histopathological examination.…”

Section: Immunohistochemical Assessment Of Inossupporting

confidence: 58%

H2S attenuates acute lung inflammation induced by administration of lipopolysaccharide in adult male rats

Ali¹,

Abdel-Hamid²,

Toni³

2018

gpb

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. Hydrogen sulfide (H2S) is gasotransmitter which plays an important role in human physiology. In this study, we aimed to check the effect of H2S treatment on acute lung inflammation (ALI). Thirty-six adult male albino rats were used and divided into: control group, ALI group which was intraperitoneally (i.p.) injected with lipopolysaccharide (LPS) at a dose of 5 mg/kg body weight, ALI group treated by the H2S donor; sodium hydrosulfide (NaHS) at a dose of 10 mg/kg body weight i.p. and ALI group treated by i.p. injection of 80 mg/kg body weight DL- propargylglycine (PAG) which is an inhibitor of endogenous H2S synthesis. Serum was obtained to determine interleukin-6 (IL-6) levels. Lipid peroxides and total antioxidant capacity (TAC) levels were measured in lung. Lung histopathology and expression of inducible nitric oxide synthase (iNOS) were also done. Results showed that NaHS improved lung inflammation through its inhibitory effect on iNOS expression, decreasing the levels of IL-6 and lipid peroxides and increasing TAC levels. But, ALI was exacerbated with PAG administration. In conclusion, the results proved that H2S has a protective effect against LPS induced ALI due to its anti-nitrative, anti-oxidant and anti-inflammatory properties.

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Section: Immunohistochemical Assessment Of Inossupporting

confidence: 58%

H2S attenuates acute lung inflammation induced by administration of lipopolysaccharide in adult male rats

Ali¹,

Abdel-Hamid²,

Toni³

2018

gpb

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“…In traditional Chinese medicine, M. alba leaves have been used to treat fever and cold for thousands of years. It has been reported to exhibit various biological activities, including anti-inflammatory, neuroprotection, antioxidant, anti-Alzheimer’s, and anti-diabetic effects [ 8 , 9 , 10 , 11 , 12 ]. Recently, extracts from M. alba leaves have been shown to inhibit melanogenesis in mushroom tyrosinase activity assays [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Anti-Melanogenesis Constituents from Morus alba L. Leaves

Park

et al. 2018

Molecules

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The individual parts of Morus alba L. including root bark, branches, leaves, and fruits are used as a cosmetic ingredient in many Asian countries. This study identified several anti-melanogenesis constituents in a 70% ethanol extract of M. alba leaves. The ethyl acetate fraction of the initial ethanol extract decreased the activity of tyrosinase, a key enzyme in the synthetic pathway of melanin. Twelve compounds were isolated from this fraction and their structures were identified based on spectroscopic spectra. Then, the authors investigated the anti-melanogenesis effects of the isolated compounds in B16-F10 mouse melanoma cells. Compounds 3 and 8 significantly inhibited not only melanin production but also intracellular tyrosinase activity in alpha-melanocyte-stimulating-hormone (α-MSH)-induced B16-F10 cells in a dose-dependent manner. These same compounds also inhibited melanogenesis-related protein expression such as microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related protein-1 (TRP-1). Compound 3 modulated the cAMP-responsive element-binding protein (CREB) and p38 signaling pathways in α-MSH-activated B16-F10 melanoma cells, which resulted in the anti-melanogenesis effects. These results suggest that compound 3, isolated from M. alba leaves, could be used to inhibit melanin production via the regulation of melanogenesis-related protein expression.

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“…The leaf of plant, Mori folium (Supplementary Material, Figure S1), has been used in traditional medicine for the treatment of various diseases (Yang et al 2014, Chan et al 2016). Mori folium exhibits a variety of pharmacological activities, such as anti-microbial (Tirupathi et al 2011), antitumor (Deepa et al 2013), anti-obesity (Sugimoto et al 2009, Ann et al 2015), anti-hypotensive (Kobayashi et al 2010, neuroprotective (Xiang et al 2010), anti-diabetic (Naowaboot et al 2009, and immunomodulatory potentials (Kwon et al 2016). In addition, Mori folium possesses free radical-scavenging activities (Kim and Jang 2011, Iqbal et al 2012, Raman et al 2016, which may explain its antioxidant ability (Khan et al 2013, Raman et al 2016.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish

Kwon

Jeong

Choi

et al. 2017

An. Acad. Bras. Ciênc.

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Mori folium, the leaf of Morus alba L. (Moraceae), has been traditionally used for various medicinal purposes from ancient times to the present. In this study, we examined the effects of water extract of Mori folium (WEMF) on the production of inflammatory mediators, such as nitric oxide (NO) and prostaglandin E 2 (PGE 2 ), and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. Our data indicated that WEMF significantly suppressed the secretion of NO and PGE 2 in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects were accompanied by a marked reduction in their regulatory gene expression at the transcription level. WEMF attenuated LPSinduced intracellular ROS production in RAW 264.7 macrophages. It inhibited the nuclear translocation of the nuclear factor-kappa B p65 subunit and the activation of mitogen-activated protein kinases in LPStreated RAW 264.7 macrophages. Furthermore, WEMF reduced LPS-induced NO production and ROS accumulation in zebrafish. Although more efforts are needed to fully understand the critical role of WEMF in the inhibition of inflammation, the findings of the present study may provide insights into the approaches for Mori folium as a potential therapeutic agent for inflammatory and antioxidant disorders.

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The Immunomodulatory Activity of Mori folium, the Leaf of Morus alba L., in RAW 264.7 Macrophages In Vitro

Cited by 25 publications

References 41 publications

H2S attenuates acute lung inflammation induced by administration of lipopolysaccharide in adult male rats

H2S attenuates acute lung inflammation induced by administration of lipopolysaccharide in adult male rats

Identification of Anti-Melanogenesis Constituents from Morus alba L. Leaves

Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish

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