The Immunological Properties of Stroma-free Polyhemolysate Containing Catalase and Superoxide Dismutase Activities Prepared by Polymerized Bovine Stroma-free Hemolysate

Abstract

Crosslinking of ultrapure hemoglobin, crystalline catalase, and superoxide dismutase resulted in a soluble nanodimensional complex of polyhemoglobin-catalase-superoxide dismutase. A less expensive and more convenient way is to crosslink bovine stromafree hemolysate (stroma-free hemolysate) that already contains hemoglobin, catalase, and superoxide dismutase into polyhemoglobin with catalase and superoxide dismutase activities (stroma-free polyhemolysate) [21]. The objective of the present study is to evaluate the immunological properties of this stroma-free polyhemolysate. Each of three groups of rats received weekly subcutaneous injections of one of the stroma-free polyhemolysate, stroma-free hemolysate, and saline for four weeks. One week after the four cycles of weekly immunization, serum and plasma were collected for C3a complement activation tests and Ouchterlony antibody-antigen precipitation tests, respectively. Results show that stroma-free polyhemolysate retained signifi cant antioxidant enzyme activity. The C3a complement activation test and Ouchterlony test show that four weekly subcutaneous injections of bovine stroma-free polyhemolysate did not result in any immunological reaction in rats when tested this way

pPolyHb protects myocardial H9C2 cells against ischemia-reperfusion injury by regulating the Pink1-Parkin-mediated mitochondrial autophagy pathway

“…The porcine Hb cross-linked by glutaraldehyde (Lifegen 100) was prepared as described previously, with certain modifications [14,15] and was a kind gift from Lifegen Co. Ltd. (Xi'an, Shaanxi, China) Briefly, Hb from fresh porcine blood was purified through specific steps and then cross-linked by glutaraldehyde. Small molecules, including excess glutaraldehyde and tetrameric haemoglobin, were removed by ultrafiltration.…”

Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports Both Oxygen and Carbon Dioxide and Also Acts as an Antioxidant

“…An experiment simulating more physiological conditions was carried out to further investigate the effect of the buffering characteristics of Hb. In the plasma, the levels of bicarbonate and carbonate are present in the range of 23-30 mM [16]. A solution of PolySFHb was thus tested for CO 2 hydration activity using buffers at physiological concentrations (Fig.…”

Extraction of superoxide dismutase, catalase, and carbonic anhydrase from stroma-free red blood cell hemolysate for the preparation of the nanobiotechnological complex of polyhemoglobin–superoxide dismutase–catalase–carbonic anhydrase

“…when tested in rats (Zhu et al 2010). We shall investigate the eff ects of using higher concentrations of enzymes.…”