The immune system of sea bass, Dicentrarchus labrax, reared in aquaculture

Scapigliati, Giuseppe; Romano, Nicla; Buonocore, Francesco; Picchietti, Simona; Baldassini, Maria Rosaria; Prugnoli, Daniela; Galice, A.; Meloni, Sabrina; Secombes, Christopher J.; Mazzini, Massimo; Abelli, Luigi

doi:10.1016/s0145-305x(01)00057-x

Cited by 49 publications

(30 citation statements)

References 58 publications

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“…3 (continued ) observed no difference in the distribution of the Ig-synthesizing cells in HK or spleen when comparing immunised and non-immunised Atlantic cod. Plasma cells have been shown to be present in PBL of carp [43] and sea bass [44,45] and in salmon strong cytoplasmic staining of some Igþ cells, presumably plasma cells, has been observed [35]. These findings imply that some teleosts may possess circulating plasma cells.…”

Section: Discussionmentioning

confidence: 90%

Neutrophils and B-cells in Atlantic cod (Gadus morhua L.)

Rønneseth

Wergeland

Pettersen

2007

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 90%

Neutrophils and B-cells in Atlantic cod (Gadus morhua L.)

Rønneseth

Wergeland

Pettersen

2007

Fish & Shellfish Immunology

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“…As a result, many studies have been focused on the physiology of reproduction, feeding, metabolism and photoperiodism in this species [Carrillo et al, 1995;Sánchez-Vázquez et al, 1995;Felip et al, 1999;Scapigliati et al, 2002;Bayarri et al, 2003Bayarri et al, , 2004aRodríguez et al, 2004;Piferrer et al, 2005;Migaud et al, 2007]. Recently, it has been reported that the organization of the circadian system in sea bass is different from that of other fish because both the pineal organ and the retina are essential to sustain nocturnal melatonin synthesis in this species [Bayarri et al, 2003;Migaud et al, 2007].…”

mentioning

confidence: 99%

Afferent and Efferent Connections of the Pineal Organ in the European Sea Bass <i>Dicentrarchus labrax:</i> A Carbocyanine Dye Tract-Tracing Study

Servili

Herrera-Pérez²,

Yáñez³

et al. 2011

Brain Behav Evol

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The pineal organ of fish is a photosensitive structure that receives light information from the environment and transduces it into hormonal (rhythmic melatonin secretion) and neural (efferent projections/neurotransmitters) signals. In this study, we focused on this neural output. Thus, we performed a tract-tracing study using 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), a fluorescent carbocyanine dye, in order to elucidate the efferent and afferent connections of the pineal organ in the European sea bass. The axonal transport of DiI revealed extensive bilateral projections in the sea bass brain. The efferent projections of the sea bass pineal organ reach the habenula, ventral thalamus, periventricular pretectum, central pretectal area, posterior tubercle and medial and dorsal tegmental areas. In addition, in this study we also examined the pinealopetal system in sea bass. This analysis demonstrated that the sea bass pineal organ receives central projections from neurons located, to a large extent, in brain areas innervated by pineal efferent projections, i.e. the thalamic eminence, habenula, ventral thalamus, dorsal thalamus, periventricular pretectum, posterior commissure, posterior tubercle and medial tegmental area. This study is the first description of pinealofugal projections in a representative of Perciformes, which constitutes a derived order within teleosts. Moreover, it represents the first evidence for the presence of pinealopetal neurons in the brain of a teleost species. Our findings, together with the analysis of retinal connections, represent a step forward in the understanding of the integration of photoperiodic signals into the central nervous system of sea bass.

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“…MHC class I and II, TcRβ and numerous interleukins have been previously demonstrated in numerous fish species (for reviews, see Stet et al 1996;Charlemagne et al 1998;Hansen and Strassburger 2000) including sea bass (Scapigliati et al 2002). Recently, the TcR co-receptor CD8α has been identified in trout (Hansen and Strassburger 2000;Stuge et al 2000) indicating that at least some of the fish T-lymphocytes can exert mechanisms of cytotoxicity as described in mammals.…”

Section: Discussionmentioning

confidence: 97%

In vivo allograft rejection in a bony fish Dicentrarchus labrax (L.): characterisation of effector lymphocytes

et al. 2005

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Lymphoid cell subpopulations involved in allograft rejection in the teleost Dicentrarchus labrax were characterised at the ultrastructural level and quantified by using monoclonal antibodies against T- and B-lymphocytes. T-cells positive for T-cell receptor beta-chain (TcR beta) were detected by reverse transcription/polymerase chain reaction (RT-PCR) and in situ hybridisation by using RNA probes for TcR beta. Flow cytometry detected a similar percentage of T- and B-lymphocytes (around 17%) in the leucocyte-enriched fraction from allografts. Two different types of T-lymphocytes (DLT 15-immunoreactive) infiltrating the allografts were identified by cytomorphology: small cells with high nuclear/cytoplasmic ratio and cells with a higher cytoplasmic content. RT-PCR revealed a single band (513 bp) corresponding to the TcR beta. In situ hybridisation showed that TcR beta-positive cells in the grafted muscle fibres were less numerous compared with DLT 15-positive cells, as evidenced in parallel sections, suggesting that cytotoxic cells might express different TcR phenotypes. DLIg 3-immunoreactive Ig-producing lymphocytes had: 1) a high nuclear/cytoplasmic ratio or 2) a larger size similar to that of pre-plasma cells (plasma cells lacked any membrane labelling).

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The immune system of sea bass, Dicentrarchus labrax, reared in aquaculture

Cited by 49 publications

References 58 publications

Neutrophils and B-cells in Atlantic cod (Gadus morhua L.)

Neutrophils and B-cells in Atlantic cod (Gadus morhua L.)

Afferent and Efferent Connections of the Pineal Organ in the European Sea Bass <i>Dicentrarchus labrax:</i> A Carbocyanine Dye Tract-Tracing Study

In vivo allograft rejection in a bony fish Dicentrarchus labrax (L.): characterisation of effector lymphocytes

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