In vivo allograft rejection in a bony fish Dicentrarchus labrax (L.): characterisation of effector lymphocytes

Romano, Nicla; Baldassini, Maria Rosaria; Buonocore, Francesco; Picchietti, Simona; Mastrolia, Lucia; Abelli, Luigi

doi:10.1007/s00441-005-1137-1

Cited by 13 publications

(5 citation statements)

References 41 publications

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“…Interestingly, peripheral blood leucocytes from fish immunized with a DNA vaccine encoding the VHSV G protein lysed efficiently VHSV infected but not infectious haematopoietic necrosis virus (IHNV)-infected histocompatible targets [116]. Cytotoxic T cells were studied in sea bass, where TRB mRNA were detected by in situ hybridization in effector lymphocytes involved in allograft rejection [117]. In this species, cytotoxic T cells seem to be involved in mixed leucocyte reaction [118].…”

Section: T Cell-mediated Cytotoxicitymentioning

confidence: 99%

T cell diversity and TcR repertoires in teleost fish

Castro

Bernard

Lefranc

et al. 2011

Fish & Shellfish Immunology

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Section: T Cell-mediated Cytotoxicitymentioning

confidence: 99%

T cell diversity and TcR repertoires in teleost fish

Castro

Bernard

Lefranc

et al. 2011

Fish & Shellfish Immunology

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“…Romano et al [ 62 ] further characterized the effector cells involved in allograft rejection in sea bass employing electron microscopy combined with FACS, RT-PCR analysis, and ISH. Two different types of T-lymphocytes (DLT15-immunoreactive) infiltrating the allografts were identified and TcRβ + cells in the graft were less numerous compared with DLT15-positive cells.…”

Section: Function Of Fish T Cellsmentioning

confidence: 99%

T Cells in Fish

Nakanishi

Shibasaki

Matsuura

2015

Biology

130

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Cartilaginous and bony fish are the most primitive vertebrates with a thymus, and possess T cells equivalent to those in mammals. There are a number of studies in fish demonstrating that the thymus is the essential organ for development of T lymphocytes from early thymocyte progenitors to functionally competent T cells. A high number of T cells in the intestine and gills has been reported in several fish species. Involvement of CD4+ and CD8α+ T cells in allograft rejection and graft-versus-host reaction (GVHR) has been demonstrated using monoclonal antibodies. Conservation of CD4+ helper T cell functions among teleost fishes has been suggested in a number studies employing mixed leukocyte culture (MLC) and hapten/carrier effect. Alloantigen- and virus-specific cytotoxicity has also been demonstrated in ginbuna and rainbow trout. Furthermore, the important role of cell-mediated immunity rather than humoral immunity has been reported in the protection against intracellular bacterial infection. Recently, the direct antibacterial activity of CD8α+, CD4+ T-cells and sIgM+ cells in fish has been reported. In this review, we summarize the recent progress in T cell research focusing on the tissue distribution and function of fish T cells.

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“…For the construction of digoxigenin (DIG)-labelled RNA TCR probes, the 513-bp PCR product was used as already described [15].…”

Section: Quantification Of Cdnamentioning

confidence: 99%

Lymphocyte differentiation in sea bass thymus: CD4 and CD8-α gene expression studies

Picchietti

Guerra

Buonocore

et al. 2009

Fish & Shellfish Immunology

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Different developmental stages (from eggs to 1-year-old juveniles) of the teleost fish Dicentrarchus labrax (L.) were assayed for CD4 gene expression. RT-PCR revealed the appearance of CD4 transcripts in post-larvae from 51 days post-hatching (dph). This finding overlaps the first detection of CD8-alpha mRNA. Real-time PCR with specific primers quantified CD4, CD8-alpha and TCR-beta transcripts in larvae and post-larvae (25, 51, 75 and 92 dph) and 1-year-old thymus. At 92 dph, TcR-beta and CD8-alpha transcripts were significantly higher (P < 0.001) than in previous stages, as CD4 transcripts compared with 51 dph (P < 0.01). High levels of TCR-beta and CD8-alpha transcripts were found in the thymus, while CD4 transcripts were lower (P < 0.05 vs. TCR-beta). In situ hybridization identified CD4 mRNAs at 51 dph, localized in thymocytes of the outer and lateral zones of the thymic glands. From 75 dph on the signal was mainly detected in the outer region, drawing a cortex-medulla demarcation. Developmental expression of CD4 and CD8-alpha almost coincided. In each adult thymic lobe CD4(+) and CD8-alpha(+) thymocytes filled the cortex. The expression patterns of CD4 and CD8-alpha largely overlap, except in the medulla, where CD4(+) thymocytes were isolated, while CD8-alpha(+) ones mainly arranged in cords. These results provide new information about the thymic compartmentalization and lymphocyte differentiation pathways in a teleost, almost demonstrating that double negative thymocytes fill the cortex giving rise to further selection in the medulla.

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In vivo allograft rejection in a bony fish Dicentrarchus labrax (L.): characterisation of effector lymphocytes

Cited by 13 publications

References 41 publications

T cell diversity and TcR repertoires in teleost fish

T cell diversity and TcR repertoires in teleost fish

T Cells in Fish

Lymphocyte differentiation in sea bass thymus: CD4 and CD8-α gene expression studies

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