The immune response to infection with vaccinia virus in mice: I. Infection and the production of antibody neutralizing cell-associated and cell-free virus

Hutt, Lindsey M.

doi:10.1017/s0022172400046829

Cited by 12 publications

(11 citation statements)

References 32 publications

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“…Antibody titers then increase and remain at high concentrations through at least 20 days. The kinetics of development of neutralizing antibodies detected here are similar to that presented by Hutt [14] who used a different route of inoculation. Thus both routes of inoculation appear to lead to the same neutralizing antibody response.…”

Section: Discussionsupporting

confidence: 86%

“…This level is consistent through 20 days after infection. These results are strikingly similar to those of Hutt [14], who used an intravenous route of infection for her studies. These neutralization experiments utilized purified intracellular vaccinia virus (INV) as the antigen.…”

Section: Appearance Of Neutralizing Antibodysupporting

confidence: 87%

“…The immune response to vaccinia virus infection includes specific and nonspecific cellular responses which involve macrophages [5], natural killer (NK) cells [3,4,30], and cytotoxic T lymphocytes [16][17]18], a humoral response [1,14,26], and other factors such as interferon production [6,9]. While all factors may play a role in recovery of the host from a primary infection, the most important factor has always been considered to be the virus-specific cytotoxic T lymphocyte (CTL) response [10].…”

Section: Introductionmentioning

confidence: 99%

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The immune response to vaccinia virus infection in mice: analysis of the role of antibody

Novembre

Raska²,

Holowczak³

1989

Archives of Virology

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Immune response to primary intraperitoneal infection with vaccinia virus (strain IHD-J) was studied in C3H/Hej mice. Antibodies reactive with virus structural proteins were detected 6 days and neutralizing antibodies 8 days after infection. Although serum antibodies from infected mice bound to vaccinia virus infected cells, these antibodies were ineffective in complement mediated lysis of infected cells and were only moderately active in experiments with antibody dependent cellular cytotoxicity (ADCC). Immunoblotting analysis showed that serum antibody reacted with a number of structural proteins of both intracellular and extracellular forms of vaccinia virus. Immunoprecipitation results showed antibody binding of nonstructural proteins and glycoproteins. Correlation of the kinetics of NK and CTL activities in infected mice with neutralizing antibodies indicated that the cellular functions clearly precede the appearance of serum neutralizing antibody. The resolution of primary infection in mice thus appears to be mediated by functions of cellular immunity while resistance to reinfection may be dependent on circulating neutralizing antibody.

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Section: Discussionsupporting

confidence: 86%

Section: Appearance Of Neutralizing Antibodysupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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The immune response to vaccinia virus infection in mice: analysis of the role of antibody

Novembre

Raska²,

Holowczak³

1989

Archives of Virology

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“…Further experiments are needed to achieve the optimal regime. In addition, while pock lesions have been reported following vaccinia virus administration to nude mice 43 - 45 , they have not been observed in immunocompetent mice 44 - 47 . Therefore, further studies are needed to clarify our findings using immunocompetent tumor models, where the potential for systemic treatment may not be hampered by systemic side effects.…”

Section: Discussionmentioning

confidence: 93%

Lister strain of vaccinia virus armed with endostatin–angiostatin fusion gene as a novel therapeutic agent for human pancreatic cancer

Tysome¹,

Briat²,

Alusi³

et al. 2009

Gene Ther

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SummarySurvival following pancreatic cancer remains poor despite incremental advances in surgical and adjuvant therapy, and new strategies for treatment are needed. Oncolytic virotherapy is an attractive approach for cancer treatment. In this study, we have evaluated the effectiveness of the Lister vaccine strain of vaccinia virus armed with the endostatin-angiostatin fusion gene (VVhEA) as a novel therapeutic approach for pancreatic cancer. The Lister vaccine strain of vaccinia virus was effective against all human pancreatic carcinoma cells tested in vitro, especially those insensitive to oncolytic adenovirus. The virus displayed inherently high selectivity for cancer cells, sparing normal cells both in vitro and in vivo, with effective infection of tumors after both intravenous (IV) and intratumoral (IT) administration. The expression of endostatin-angiostatin fusion protein was confirmed in a pancreatic cancer model both in vitro and in vivo, with evidence of inhibition of angiogenesis. This novel vaccinia virus demonstrated significant antitumor potency in vivo against the Suit-2 model by IT administration. The present study suggests that the novel Lister strain of vaccinia virus armed with the endostatin-angiostatin fusion gene is a potential therapeutic agent for pancreatic cancer.

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“…The ability of TB-mice to mount such a response indicated that the animals were potentially capable of eliminating virus-infected cells as efficiently as animals without tumors [8]. We confirmed this by directly titrating the amounts of virus in various organs [15] (spleen, liver and, when present, the tumor nodule) following challenge with vaccinia virus (data not shown). While infection of cells in the tumor mass did occur, in no case could we measure a significant reduction in the size of tumor nodules as a result of such infection.…”

Section: Discussionmentioning

confidence: 52%

Immune responses of DBA/2 mice bearing melanoma tumors: Cell-mediated immune responses after challenge with vaccinia virus

Byrne

Soloski

Holowczak

1983

Cancer Immunol Immunother

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Cell-mediated immune responses in DBA/2 mice bearing melanoma tumors (TB-mice) were measured and compared to similar responses in mice without tumors (C-mice). Splenic lymphocytes from TB-mice had a reduced capacity to respond to both B and T-cell mitogens, but TB-mice responded to infection with vaccinia virus by developing a virus-specific cytotoxic T-cell response equal to that measured with splenic effectors prepared from virus-infected C-mice. NK-cell activity, as measured by the in vitro lysis of YAC-1 targets by splenic effectors, was significantly depressed in TB-mice but, after infection of the animals with vaccinia virus, was restored to levels equal to that measured with splenic effectors prepared from C-mice. Doses of vaccinia virus, strain WR which elicited vaccinia-virus-specific cytotoxic T cells or stimulated NK-cell activity, failed to elicit or stimulate cytotoxic effectors specific for S91-melanoma tumor cells.

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The immune response to infection with vaccinia virus in mice: I. Infection and the production of antibody neutralizing cell-associated and cell-free virus

Cited by 12 publications

References 32 publications

The immune response to vaccinia virus infection in mice: analysis of the role of antibody

The immune response to vaccinia virus infection in mice: analysis of the role of antibody

Lister strain of vaccinia virus armed with endostatin–angiostatin fusion gene as a novel therapeutic agent for human pancreatic cancer

Immune responses of DBA/2 mice bearing melanoma tumors: Cell-mediated immune responses after challenge with vaccinia virus

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