The T allele at the g.1471620G>T in the EDG1 gene associated with high marbling in Japanese Black cattle is at a low frequency in breeds not selected for marbling

Abstract

Our previous study detected a single nucleotide polymorphism (SNP), g.1471620G>T, in the 5' flanking region of the endothelial differentiation sphingolipid G-protein-coupled receptor 1 (EDG1) gene, which has been considered as a positional functional candidate for the gene responsible for marbling, and showed association of the g.1471620G>T SNP with marbling in Japanese Black beef cattle. In the present study, we investigated the allele frequency distribution of the g.1471620G>T SNP among the 5 cattle breeds, Japanese Black, Japanese Brown, Japanese Short Horn, Holstein, and Brown Swiss breeds. The T allele at the g.1471620G>T SNP associated with high marbling was found at high frequency in Japanese Black breed that has been subjected to a strong selection for high marbling, while the allele was absent or at very low frequencies in the other breeds that have not been strongly selected for high marbling. Based on this finding, we hypothesized that the pressure of the strong selection for high marbling in Japanese Black breed has increased the frequency of the T allele at the g.1471620G>T SNP in the EDG1.

The The favorable alleles of AKIRIN2:c.*188G>A, EDG1:c.-312A>G and TTN:g.231054C>T as candidate markers for high-marbling are very low in Bali cattle

“…This is possible because there has never been a selection program for high-marbling in the history of Bali cattle breeding in Indonesia. Watanabe et al (2010) and Watanabe et al (2011) stated that the strong selection pressure has led to selective sweep for favorable alleles that have a direct impact on marbling including of AKIRIN2, EDG1, and TTN gene. It may also happen in Korean cattle since the favorable alleles were found in high frequency.…”

SNPs in AKIRIN2, TTN, EDG1 and MYBPC1 genes are associated with growth-related traits in Chinese Qinchuan cattle

“…To further analyze the distribution of the well-characterized allele of each SNP in the cattle populations based on the different genetic background, the MGC, MGG, QC, LX, WL and LL of this study as well as the JB, JBR, JSH, HOL, BS and KN [19,[21][22][23] were divided into six groups which including North China (MGC and MGG) [20], Central China (QC and LX) [20], South China (WL and LL) [20], Japanese cattle group (JB, JBR and JSH), European cattle group (HOL and BS), and Korean cattle (KN) (Figure 2; Table 4). At the c.*188G>A locus in AKIRIN2, the well-characterized A allele frequency in the JG and KN was obviously higher than the other cattle groups ( Fig.…”

“…(a-d) The allele frequency of the c.*188G>A SNP in AKIRIN2, the g.231054C>T SNP in TTN, the g.1471620G>T SNP in EDG1, and the g.70014208A>G SNP in MYBPC1 genes, respectively. NC: North China; CC: Central China; SC: South China; JG: Japanese cattle group [21][22][23]; EC: European cattle group [21][22][23]; KN: Korean cattle [19].…”

“…Therefore, the objectives of this study reported herein were to: (1) perform association analyses between these SNPs in the AKIRIN2, TTN, EDG1 and MYBPC1 genes and growth-related traits in QC, (2) investigate the genetic diversity of these SNPs in three Chinese typical cattle groups (including QC, Mongolia cattle (MG), Luxi (LX), Wuling (WL) and Longlin (LL) cattle breeds), and (3) compare the frequencies of the well-characterized alleles of these SNPs in Chinese cattle breeds to those of JB, Japanese Brown (JBR), Japanese Short Horn (JSH), Holstein (HOL), Brown Swiss (BS) [21][22][23], and KN breeds [19].…”

Investigação de Polimorfismos nos Genes IGF2 e CYP21 em Bovinos de Raças Zebuínas e Análise das Possíveis Associações com Características de Interesse Econômico

“…A influência dos polimorfismos genéticos nos aspectos de interesse econômico em diversas espécies de animais domésticos tem sido amplamente estudada por diferentes grupos de pesquisadores pelo mundo (Angiolillo et al, 2007;Daetwyler et al, 2008;Grisolia et al, 2009(a,b); Pang et al, 2010 No início, a procura por polimorfismos funcionais estava direcionada para a região codificadora de genes responsáveis por importantes características fenotípicas (Bastos-Silveira et al, 2009;Grisolia et al, 2009a;MacLeod et al, 2010;Rincón et al, 2009). Vários SNPs vêm sendo investigados na genética animal, em especial nos bovinos (Garrick e Golden, 2009;Yamada et al, 2009;Huang et al, 2010;Pang et al, 2010;Pryce et al, 2010;Verschoor et al, 2010;Watanabe et al, 2010;Weigel et al, 2010;) e menor proporção em raças zebus (Andrea, 2007; . O uso de marcadores genéticos a partir da detecção de polimorfismos em regiões de genes relacionados a características de importância econômica pode favorecer a identificação de animais geneticamente superiores em bovinos de corte .…”