The Pax2 homolog sparkling is required for development of cone and pigment cells in theDrosophila eye

Fu, Weimin; Noll, Markus

doi:10.1101/gad.11.16.2066

Cited by 146 publications

(154 citation statements)

References 51 publications

(86 reference statements)

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“…N signaling via Su(H) has recently been shown to activate expression of D-Pax2 in cone cells (37); however, cone cell development in D-pax2 mutants is abnormal but not eliminated (42). E(spl)bHLH proteins are also expressed in cone cells, and we observe that this expression [as well as other aspects of retinal E(spl)bHLH expression] is Su(H)-dependent.…”

Section: Discussion Neur Is Required For a Subset Of N-dependent Procmentioning

confidence: 56%

neuralized is essential for a subset of Notch pathway-dependent cell fate decisions during Drosophila eye development

Lai

Rubin

2001

Proc. Natl. Acad. Sci. U.S.A.

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neuralized (neur) is a neurogenic mutant of Drosophila in which many signaling events mediated by the Notch (N) receptor are disrupted. Here, we analyze the role of neur during eye development. Neur is required in a cell-autonomous fashion to restrict R8 and other photoreceptor fates and is involved in lateral inhibition of interommatidial bristles but is not required for induction of the cone cell fate. The latter contrasts with the absolute requirement for Suppressor of Hairless and the Enhancer of split-Complex for cone cell induction. Using gain-of-function experiments, we further demonstrate that ectopic wild-type and truncated Neur proteins can interfere with multiple N-controlled aspects of eye development, including both neur-dependent and neur-independent processes. The Drosophila eye has been a particularly useful model system for studies of cell-cell interactions during formation of a biological pattern. The adult eye consists of some 800 identical ommatidia; each is a complete unit eye containing 20 cells, including 8 photoreceptors and 12 accessory cells. The proper commitment of cell fates in the eye depends on both inhibitory and inductive cell-cell interactions mediated by multiple signaling cascades (1).The Notch (N) receptor is directly involved in the determination of all cell types in the Drosophila eye, in addition to having other functions in controlling growth and polarity of the eye (2-6). N has two opposing functions with regard to early eye neurogenesis. It first promotes R8 photoreceptor differentiation by enhancing expression of the proneural gene for photoreceptors, atonal (7,8). R8 is the first cell fate to differentiate in the eye and is required for recruitment of all other photoreceptors; N clones lack photoreceptors (8,9). N is subsequently involved in restriction of the R8 fate, because a temporally restricted reduction in N function results in the differentiation of clusters of ectopic R8 cells (10). Other experiments performed during larval and pupal development further demonstrated that N is involved in the determination of outer photoreceptors, cone cells, pigment cells, and bristles (2). N thus appears to be required for all stages of ommatidial assembly.A central ''core'' of proteins involved in the N pathway appears to be deployed in a wide variety of settings of N-pathway activity in invertebrates as well as vertebrates (11). The basic scaffold of the N pathway, as it is most often used, appears to be as follows. Interaction of the transmembrane ligand Delta with the transmembrane receptor Notch results in the release of a proteolytic fragment including the intracellular domain of Notch (N IC ) (12)(13)(14). N IC then translocates to the nucleus, where it acts as a coactivator for the sequence-specific DNA-binding protein Suppressor of Hairless [Su(H)] (15, 16). This complex activates transcription of various target genes, which include multiple members of the Enhancer of split-Complex [E(spl)-C] (17-21).Mutations in neuralized (neur) result in a variety of developmenta...

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Section: Discussion Neur Is Required For a Subset Of N-dependent Procmentioning

confidence: 56%

neuralized is essential for a subset of Notch pathway-dependent cell fate decisions during Drosophila eye development

Lai

Rubin

2001

Proc. Natl. Acad. Sci. U.S.A.

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“…In turn, D-Pax2 is also necessary for BarH1 expression in 1°PCs. However, D-Pax2 does not help to specify 1°P C fate, because it is not expressed in undifferentiated cells (Fu and Noll, 1997). D-Pax2 mutants have fewer 2°a nd 3°PC fates, but it is not expressed in these cells, so this is most likely a non-cell autonomous effect stemming from the loss of 1°PCs (Miller and Cagan, 1998).…”

Section: Pcsmentioning

confidence: 99%

Signal integration during development: Insights from the Drosophila eye

Voas

Rebay

2003

Developmental Dynamics

162

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The Drosophila eye is a highly ordered epithelial tissue composed of ϳ750 subunits called ommatidia arranged in a reiterated hexagonal pattern. At higher resolution, observation of the constituent photoreceptors, cone cells, and pigment cells of the eye reveals a highly ordered mosaic of amazing regularity. This relatively simple organization belies the repeated requirement for spatially and temporally coordinated inputs from the Hedgehog (Hh), Wingless (Wg), Decapentaplegic (Dpp), JAK-STAT, Notch, and receptor tyrosine kinase (RTK) signaling pathways. This review will discuss how signaling inputs from the Notch and RTK pathways, superimposed on the developmental history of a cell, facilitate context-specific and appropriate cell fate specification decisions in the developing fly eye. Lessons learned from investigating the combinatorial signal integration strategies underlying Drosophila eye development will likely reveal cell-cell communication paradigms relevant to many aspects of invertebrate and mammalian development. Developmental Dynamics 229:162-175, 2004.

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“…Expression of both D. melanogaster Pax2/ 5/8 orthologues, Pox neuro (Poxn) and Pax2, is present at the interface of otd and the Gbx2 orthologue unplugged (unpg), anterior to a Hox-expressing region (Noll, 1993;Fu and Noll, 1997;Hirth et al, 2003). The expression domains of Poxn and Pax2 span the whole embryonic CNS in a segmentally reiterated pattern, but the genes are never coexpressed in the same cells.…”

Section: Functional Conservation Of Otd/otx Genes In Embryonic Brain mentioning

confidence: 99%

Insights into the urbilaterian brain: conserved genetic patterning mechanisms in insect and vertebrate brain development

2005

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Recent molecular genetic analyses of Drosophila melanogaster and mouse central nervous system (CNS) development revealed strikingly similar genetic patterning mechanisms in the formation of the insect and vertebrate brain. Thus, in both insects and vertebrates, the correct regionalization and neuronal identity of the anterior brain anlage is controlled by the cephalic gap genes otd/Otx and ems/Emx, whereas members of the Hox genes are involved in patterning of the posterior brain. A third intermediate domain on the anteroposterior axis of the vertebrate and insect brain is characterized by the expression of the Pax2/5/8 orthologues, suggesting that the tripartite ground plans of the protostome and deuterostome brains share a common evolutionary origin. Furthermore, cross-phylum rescue experiments demonstrate that insect and mammalian members of the otd/Otx and ems/Emx gene families can functionally replace each other in embryonic brain patterning. Homologous genes involved in dorsoventral regionalization of the CNS in vertebrates and insects show remarkably similar patterning and orientation with respect to the neurogenic region (ventral in insects and dorsal in vertebrates). This supports the notion that a dorsoventral body axis inversion occurred after the separation of protostome and deuterostome lineages in evolution. Taken together, these findings demonstrate conserved genetic patterning mechanisms in insect and vertebrate brain development and suggest a monophyletic origin of the brain in protostome and deuterostome bilaterians.

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The Pax2 homolog sparkling is required for development of cone and pigment cells in theDrosophila eye

Cited by 146 publications

References 51 publications

neuralized is essential for a subset of Notch pathway-dependent cell fate decisions during Drosophila eye development

neuralized is essential for a subset of Notch pathway-dependent cell fate decisions during Drosophila eye development

Signal integration during development: Insights from the Drosophila eye

Insights into the urbilaterian brain: conserved genetic patterning mechanisms in insect and vertebrate brain development

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