The dasABC Gene Cluster, Adjacent to dasR , Encodes a Novel ABC Transporter for the Uptake of N , N ′-Diacetylchitobiose in Streptomyces coelicolor A3(2)

Abstract

؊8 ). In the dasA-null mutant, the rate of decline of the (GlcNAc) 2 concentration in the culture supernatant was about 25% of that in strain M145. The in vitro and in vivo data clearly demonstrated that dasA is involved in (GlcNAc) 2 uptake. Upstream and downstream of dasA, the transcriptional regulator gene (dasR) and two putative integral membrane protein genes (dasBC) are located in the opposite and same orientations, respectively. The expression of dasR and dasB, which seemed independent of dasA transcription, was also induced by (GlcNAc) 2 and (GlcNAc) 3 .

The chitobiose-binding protein, DasA, acts as a link between chitin utilization and morphogenesis in Streptomyces coelicolor

“…5). In contrast to what was previously observed (Rigali et al, 2006) and as recently reported by Saito et al (2007), dasA is thus far the first known DasR-dependent gene that is not induced but rather repressed by GlcNAc.…”

“…Verification of the correct recombination events was performed by PCR and Southern hybridization. Saito et al (2007) reported that transcription of dasBC is independent of the transcription of dasA, which ensures the absence of a polar effect on the dasBC genes. For complementation of the dasA mutant we used plasmid pSET151 harbouring the dasA gene and its promoter region (nt positions 2361/+1278 relative to the start of dasA).…”

Control of chitin and N-acetylglucosamine utilization in Saccharopolyspora erythraea

“…coelicolor, the transcriptional control of genes belonging to the chitinolytic system and to GlcNAc metabolism involves the GntR-family regulatory protein DasR (Colson et al, 2007(Colson et al, , 2008Nazari et al, 2011Nazari et al, , 2013Rigali et al, 2004Rigali et al, , 2006Saito et al, 2007;Ś wiątek et al, 2012a). DasR is necessary for the proper expression of genes involved in the degradation of the polymer chitin (Nazari et al, 2011(Nazari et al, , 2013, whereas it represses the expression of genes involved in chitooligosaccharide, (GlcNAc) 2 and GlcNAc transport and catabolism (Colson et al, 2008;Rigali et al, 2006;Saito et al, 2007;Świątek et al, 2012a). Binding of GlcN-6P to the effector-binding domain of DasR impairs the DNA-binding ability of the repressor, which results in increased transcription of GlcNAcutilization genes (Rigali et al, 2006).…”

Lack of A-factor Production Induces the Expression of Nutrient Scavenging and Stress-related Proteins in Streptomyces griseus>

“…These include the universal phosphotransferase system components enzyme I, enzyme IIA Crr , and histidine phosphocarrier protein (2,36) as well as components of the DasABC transporter. DasABC transports chitobiose, the dimer of N-acetylglucosamine, and DasA is a lipoprotein that functions as the chitobiose-binding protein (39). In addition to the transport of chitobiose DasA also plays a role in nutrient sensing perhaps by interacting with the chiRS two-component system (34).…”