The <i>CLE</i> gene family in <i>Populus trichocarpa</i>

Liu, Zhijun; Yang, Nan; Lv, Yanting; Pan, Lixia; Lv, Shuo; Han, Huibin; Wang, Guodong

doi:10.1080/15592324.2016.1191734

Cited by 7 publications

(10 citation statements)

References 36 publications

(75 reference statements)

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“…Finally, the expression of many genes related to cambial growth and cell wall formation was influenced by ACC treatments (Table S10). Three PtCLE genes ( PtCLE2 , 27 , 27 ), of which two have, according to their expression, been associated with xylem tissues (Liu et al ., ), were found to be differentially regulated by ACC. Cytoskeleton‐related genes, such as two AtMAP70‐5 orthologs that are members of the actin and tubulin gene families as well as microtubule interacting proteins (TPX2 proteins) (Brunet et al ., ; Pesquet et al ., ; Goshima, ), were also affected.…”

Section: Resultsmentioning

confidence: 93%

“…Finally, the expression of many genes related to cambial growth and cell wall formation was influenced by ACC treatments (Table S10). Three PtCLE genes (PtCLE2, 27, 27), of which two have, according to their expression, been associated with xylem tissues (Liu et al, 2016), were found to be differentially regulated by ACC. Cytoskeleton-related genes, such as two Measurements were carried out from the cambium towards the pith.…”

Section: Researchmentioning

confidence: 99%

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Ethylene signaling induces gelatinous layers with typical features of tension wood in hybrid aspen

et al. 2018

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The phytohormone ethylene impacts secondary stem growth in plants by stimulating cambial activity, xylem development and fiber over vessel formation. We report the effect of ethylene on secondary cell wall formation and the molecular connection between ethylene signaling and wood formation. We applied exogenous ethylene or its precursor 1-aminocyclopropane-1-carboxylic acid (ACC) to wild-type and ethylene-insensitive hybrid aspen trees (Populus tremula × tremuloides) and studied secondary cell wall anatomy, chemistry and ultrastructure. We furthermore analyzed the transcriptome (RNA Seq) after ACC application to wild-type and ethylene-insensitive trees. We demonstrate that ACC and ethylene induce gelatinous layers (G-layers) and alter the fiber cell wall cellulose microfibril angle. G-layers are tertiary wall layers rich in cellulose, typically found in tension wood of aspen trees. A vast majority of transcripts affected by ACC are downstream of ethylene perception and include a large number of transcription factors (TFs). Motif-analyses reveal potential connections between ethylene TFs (Ethylene Response Factors (ERFs), ETHYLENE INSENSITIVE 3/ETHYLENE INSENSITIVE3-LIKE1 (EIN3/EIL1)) and wood formation. G-layer formation upon ethylene application suggests that the increase in ethylene biosynthesis observed during tension wood formation is important for its formation. Ethylene-regulated TFs of the ERF and EIN3/EIL1 type could transmit the ethylene signal.

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Section: Resultsmentioning

confidence: 93%

“…Finally, the expression of many genes related to cambial growth and cell wall formation was influenced by ACC treatments (Table S10). Three PtCLE genes (PtCLE2, 27, 27), of which two have, according to their expression, been associated with xylem tissues (Liu et al, 2016), were found to be differentially regulated by ACC. Cytoskeleton-related genes, such as two Measurements were carried out from the cambium towards the pith.…”

Section: Researchmentioning

confidence: 99%

Ethylene signaling induces gelatinous layers with typical features of tension wood in hybrid aspen

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Finally, the expression of many genes related to cambial growth and cell wall formation was influenced by ACC treatments (Table S10). Three PtCLE genes (PtCLE2, 27, 27) , of which two, have, according to their expression, been associated with xylem tissues (Liu et al , 2016), were found to be differentially regulated by ACC. Cytoskeleton related genes such as two AtMAP70-5 orthologs that are members of the actin and tubulin gene families as well as microtubule interacting proteins (TPX2 proteins) (Brunet et al , 2004; Pesquet et al , 2010; Goshima, 2011) were also affected.…”

Section: Resultsmentioning

confidence: 99%

Ethylene signaling induces gelatinous layers with typical features of tension wood in hybrid aspen

Felten

Vahala

Love

et al. 2017

Preprint

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SummaryResearch conductedThe phytohormone ethylene impacts secondary stem growth in plants by stimulating cambial activity, xylem development and fiber over vessel formation. Here we report the effect of ethylene on secondary cell wall formation and the molecular connection between ethylene signaling and wood formation.MethodsWe applied exogenous ethylene or its precursor 1-aminocyclopropane-1-carboxylic acid (ACC) to wild type and ethylene insensitive hybrid aspen trees (Populus tremula x tremuloides) and studied secondary cell wall anatomy, chemistry and ultrastructure. We furthermore analyzed the transcriptome (RNA Seq) after ACC application to wild type and ethylene insensitive trees.Key resultsWe demonstrate that ACC and ethylene induce gelatinous-layers (G-layers) and alter the fiber cell wall cellulose microfibril angle. G-layers are tertiary wall layers rich in cellulose, typically found in tension wood of aspen trees. A vast majority of transcripts affected by ACC are downstream of ethylene perception and include a large number of transcription factors (TFs). Motif-analyses reveal potential connections between ethylene TFs (ERFs, EIN3/EIL1) and wood formation.ConclusionG-layer formation upon ethylene application suggests that the increase in ethylene biosynthesis observed during tension wood formation is important for its formation. Ethylene-regulated TFs of the ERF and EIN3/EIL1 type could transmit the ethylene signal.

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“…In addition, the number of cotton CLE genes was considerably more than that seen presently in grape (nine members) (Wang et al, 2019), Arabidopsis (at least 32) (Jun et al, 2010), common bean (44) (Hastwell et al, 2015a) and rice (47) (Suzaki et al, 2006;Kinoshita et al, 2007), but less than in hexaploid bread wheat (Tricticumaestivum L.) (104) . However, the number of CLE genes (50 members) in Populus was similar to those in the A (G. arboreum) or D (G. ramondii) genomes (Liu et al, 2016). The quantity of CLE members in the allotetraploid species (G. hirsutum) was very close to that in soybean ( 84CLEs) (Hastwell et al, 2015a).…”

Section: Discussionmentioning

confidence: 80%

Molecular Traits and Functional Analysis of the CLAVATA3/Endosperm Surrounding Region-Related Small Signaling Peptides in Three Species of Gossypium Genus

et al. 2021

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The CLAVATA3/endosperm surrounding region-related (CLE) small peptides are a group of C-terminally encoded and post-translationally modified signal molecules involved in regulating the growth and development of various plants. However, the function and evolution of these peptides have so far remained elusive in cotton. In this study, 55, 56, and 86 CLE genes were identified in the Gossypium raimondii, Gossypium arboreum, and Gossypium hirsutum genomes, respectively, and all members were divided into seven groups. These groups were distinctly different in their protein characteristics, gene structures, conserved motifs, and multiple sequence alignment. Whole genome or segmental duplications played a significant role in the expansion of the CLE family in cotton, and experienced purifying selection during the long evolutionary process in cotton. Cis-acting regulatory elements and transcript profiling revealed that the CLE genes of cotton exist in different tissues, developmental stages, and respond to abiotic stresses. Protein properties, structure prediction, protein interaction network prediction of GhCLE2, GhCLE33.2, and GhCLE28.1 peptides were, respectively, analyzed. In addition, the overexpression of GhCLE2, GhCLE33.2, or GhCLE28.1 in Arabidopsis, respectively, resulted in a distinctive shrub-like dwarf plant, slightly purple leaves, large rosettes with large malformed leaves, and lack of reproductive growth. This study provides important insights into the evolution of cotton CLEs and delineates the functional conservatism and divergence of CLE genes in the growth and development of cotton.

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The CLE gene family in Populus trichocarpa

Cited by 7 publications

References 36 publications

Ethylene signaling induces gelatinous layers with typical features of tension wood in hybrid aspen

Ethylene signaling induces gelatinous layers with typical features of tension wood in hybrid aspen

Ethylene signaling induces gelatinous layers with typical features of tension wood in hybrid aspen

Molecular Traits and Functional Analysis of the CLAVATA3/Endosperm Surrounding Region-Related Small Signaling Peptides in Three Species of Gossypium Genus

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