The
            <i>Bacillus subtilis galE</i>
            Gene Is Essential in the Presence of Glucose and Galactose

Krispin, Oliver; Allmansberger, Rudolf

doi:10.1128/jb.180.8.2265-2270.1998

Cited by 36 publications

(13 citation statements)

References 24 publications

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“…In contrast, further co‐overexpression of gtaB (encoding pyrophosphorylase) diminished chondroitin accumulation (2.15 ± 0.05 g/L). The reason might be ascribed to the excessive accumulation of toxic intermediates with an unbalanced pathway (Jin, Kang, et al, ; Krispin & Allmansberger, ) or the increased flux of UDP‐Glc towards cell wall biosynthesis. Similarly, the pathway genes glmU (encoding acetyltransferase), glmM (encoding phosphoglucosamine mutase), and glmS (encoding amidotransferase) involved in UDP‐GlcNAc biosynthesis were also investigated.…”

Section: Resultsmentioning

confidence: 99%

A microbial–enzymatic strategy for producing chondroitin sulfate glycosaminoglycans

Zhou

Huang

et al. 2018

Biotech & Bioengineering

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Chondroitin sulfate has been widely used in both medical and clinical applications. Commercial chondroitin sulfate has been mainly acquired from animal tissue extraction. Here we report a new two-step biological strategy for producing chondroitin sulfate A and chondroitin sulfate C. First, the chondroitin biosynthesis pathway in a recombinant Bacillus subtilis strain using sucrose as carbon source was systematically optimized and the titer of chondroitin was significantly enhanced to 7.15 g/L. Then, specific sulfation transformation systems were successfully constructed and optimized by combining the purified aryl sulfotransferase IV (ASST IV), chondroitin 4-sulfotransferase (C4ST) and chondroitin 6-sulfotransferase (C6ST). Chondroitin sulfate A and C were enzymatically transformed from chondroitin at conversion rates of 98% and 96%, respectively. The present biological strategy has great potential to be scaled up for biosynthesis of chondroitin sulfate A and C from cheap carbon sources.

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Section: Resultsmentioning

confidence: 99%

A microbial–enzymatic strategy for producing chondroitin sulfate glycosaminoglycans

Zhou

Huang

et al. 2018

Biotech & Bioengineering

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“…B. subtilis contains the genes which encode the proteins necessary for the degradation of lactose and galactose, and these genes are expressed under inducing β-galactosidase. But the toxic effect of galactose might be occurred due to the accumulation of UDP-galactose [31]. B. siamensis SB1001 can slightly grow on lactose and galactose (Table 2), which might be affected by the toxic of UDP-galactose.…”

Section: Effect Of Carbon Sources On γ-Pga Production By B Siamensismentioning

confidence: 99%

High-level production of poly-γ-glutamic acid from untreated molasses by Bacillus siamensis IR10

et al. 2020

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Background: Poly-γ-glutamic acid (γ-PGA) is a promising biopolymer and has been applied in many fields. Bacillus siamensis SB1001 was a newly isolated poly-γ-glutamic acid producer with sucrose as its optimal carbon source. To improve the utilization of carbon source, and then molasses can be effectively used for γ-PGA production, 60 cobalt gamma rays was used to mutate the genes of B. siamensis SB1001. Results: Bacillus siamensis IR10 was screened for the production of γ-PGA from untreated molasses. In batch fermentation, 17.86 ± 0.97 g/L γ-PGA was obtained after 15 h, which is 52.51% higher than that of its parent strain. Fed-batch fermentation was performed to further improve the yield of γ-PGA with untreated molasses, yielding 41.40 ± 2.01 g/L of γ-PGA with a productivity of 1.73 ± 0.08 g/L/h. An average γ-PGA productivity of 1.85 g/L/h was achieved in the repeated fed-batch fermentation. This is the first report of such a high γ-PGA productivity. The analysis of the enzyme activities showed that they were affected by the carbon sources, enhanced ICDH and GDH, and decreased ODHC, which are important for γ-PGA production. Conclusion: These results suggest that untreated molasses can be used for economical and industrial-scale production of γ-PGA by B. siamensis IR10.

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“…The regulation gene galR encodes an activator of the galKTE and lacSZLM operons, and a negative regulator of its own expression. The gene galR is generally present in genomes holding the Leloir pathway (Table 1), except for Bacillus subtilis 168 and S. gordonii CH1 which are unable to utilize lactose and galactose via the Leloir pathways (Krispin & Allmansberger, 1998;Zeng et al, 2012). However, the presence an intanct copy the gene galR does not necessarily lead to a galactose fermenting phenotype.…”

Section: Regulation Of the Leloir Pathway Genesmentioning

confidence: 99%

Review of lactose and galactose metabolism in Lactic Acid Bacteria dedicated to expert genomic annotation

Iskandar

Cailliez‐Grimal

Borges

et al. 2019

Trends in Food Science & Technology

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The Bacillus subtilis galE Gene Is Essential in the Presence of Glucose and Galactose

Cited by 36 publications

References 24 publications

A microbial–enzymatic strategy for producing chondroitin sulfate glycosaminoglycans

A microbial–enzymatic strategy for producing chondroitin sulfate glycosaminoglycans

High-level production of poly-γ-glutamic acid from untreated molasses by Bacillus siamensis IR10

Review of lactose and galactose metabolism in Lactic Acid Bacteria dedicated to expert genomic annotation

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