The Arabidopsis male-sterile mutant, opr3 , lacks the 12-oxophytodienoic acid reductase required for jasmonate synthesis

Abstract

Jasmonic acid (JA) and its precursor 12-oxophytodienoic acid (OPDA) act as plant growth regulators and mediate responses to environmental cues. To investigate the role of these oxylipins in anther and pollen development, we characterized a T-DNA-tagged, male-sterile mutant of Arabidopsis, opr3. The opr3 mutant plants are sterile but can be rendered fertile by exogenous JA but not by OPDA. Cloning of the mutant locus indicates that it encodes an isozyme of 12-oxophytodienoate reductase, designated OPR3. All of the defects in opr3 are alleviated by transformation of the mutant with an OPR3 cDNA. Our results indicate that JA and not OPDA is the signaling molecule that induces and coordinates the elongation of the anther filament, the opening of the stomium at anthesis, and the production of viable pollen. Just as importantly, our data demonstrate that OPR3 is the only isoform of OPR capable of reducing the correct stereoisomer of OPDA to produce JA required for male gametophyte development.

High-resolution fine mapping of ps-2, a mutated gene conferring functional male sterility in tomato due to non-dehiscent anthers

“…In addition the sequence of the ps-2 gene will give us more insights into the physiology of anther dehiscence in tomato. Similar phenotypes have been observed in some Arabidopsis mutants where, in most of the cases, the jasmonic acid pathway was involved (Feys et al 1994;Ishiguro et al 2001;von Malek et al 2002;McConn and Browse 1996;Park et al 2002;Sanders et al 2000;Stintzi and Browse 2000;Xie et al 1998). Once the ps-2 gene is cloned, it will be interesting to know whether this gene is ortholog to one of those genes already identiWed in Arabidopsis.…”

“…The molecular mechanism of ps-2 in tomato is unknown, but similar mutant phenotypes have been observed and studied mainly in Arabidopsis, such as the mutant myb26 (Steiner-Lange et al 2003), the mutants coi1 (Feys et al 1994;Xie et al 1998) and dad1 (Ishiguro et al 2001), the double mutants opr3/ dde1 (Sanders et al 2000;Stintzi and Browse 2000) and aos/dde2-2 (von Malek et al 2002;Park et al 2002) and the triple mutant fad3/fad7/fad8 (McConn and Browse 1996). They are characterized by defects in Wlament elongation, timing of anther dehiscence and often show reduced pollen viability.…”

“…All, except the mutant myb26, are in one way or another involved in the jasmonic acid pathway. The mutant coi1 (Xie et al 1998) is insensitive to jasmonic acid, while the others are defective in jasmonic acid synthesis (Feys et al 1994;Ishiguro et al 2001;McConn and Browse 1996;Park et al 2002;Sanders et al 2000;Stintzi and Browse 2000;von Malek et al 2002). Atanassova (1999) observed up to 26% self-pollination in the most extreme cases, on cultivar "Vrbicanske nizke" (ps-2/ps-2) under Weld conditions.…”

Functional conservation and maintenance of expression pattern of FIDDLEHEAD-like genes in Arabidopsis and Antirrhinum

“…Several dehiscence mutants are available in Arabidopsis (Sanders et al, 1999) that could be helpful in establishing the role of the FDH gene in the process of cell separation within the stomium. Like FDH, the anther dehiscence genes that have so far been identified molecularly, DEFECTIVE IN ANTHER DEHISCENCE 1 (DAD1) (Ishiguro et al, 2001), DELAYED DEHISCENCE 1 (Sanders et al, 2000) and OPR3 (Stintzi and Browse, 2000), all encode enzymes of lipid metabolism.…”

SUMO proteases OTS1 and 2 control filament elongation through a DELLA-dependent mechanism

“…Reduced filament elongation is also associated with defects in the Jasmonate (JA) pathway (Stintzi and Browse 2000). GA and JA signalling pathways exhibit different points of interactions through DELLA (Cheng et al 2009;Hou et al 2010;Qi et al 2014).…”