SUMO proteases OTS1 and 2 control filament elongation through a DELLA-dependent mechanism

Campanaro, Alberto; Battaglia, Raffaella; Galbiati, Massimo; Sadanandom, Ari; Tonelli, Chiara; Conti, Lucio

doi:10.1007/s00497-016-0292-8

Cited by 18 publications

(13 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ots1-1 ots2-1 double mutants also produce a lower number of seeds and have a significantly reduced filament length. Some of the observed phenotypes in ots1-1 ots2-1 double mutant are reversed to WT conditions if either a DELLA protein is also knocked out from the mutant such as RGA (repressor of ga1-3) or GAI (gibberellin-insensitive), or if GA (gibberellins) or JA (jasmonic acid) is applied (Campananaro et al, 2016). This suggests that the fertility phenotype of ots1-1 ots2-1 is controlled through the DELLA proteins (Campananaro et al, 2016).…”

Section: Physiological Effect Of Sumo Proteasesmentioning

confidence: 99%

Dealing With Stress: A Review of Plant SUMO Proteases

Morrell

Sadanandom

2019

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

The SUMO system is a rapid dynamic post-translational mechanism employed by eukaryotic cells to respond to stress. Plant cells experience hyperSUMOylation of substrates in response to stresses such as heat, ethanol, and drought. Many SUMOylated proteins are located in the nucleus, SUMOylation altering many nuclear processes. The SUMO proteases play two key functions in the SUMO cycle by generating free SUMO; they have an important role in regulating the SUMO cycle, and by cleaving SUMO off SUMOylated proteins, they provide specificity to which proteins become SUMOylated. This review summarizes the broad literature of plant SUMO proteases describing their catalytic activity, domains and structure, evolution, localization, and response to stress and highlighting potential new areas of research in the future.

show abstract

Section: Physiological Effect Of Sumo Proteasesmentioning

confidence: 99%

Dealing With Stress: A Review of Plant SUMO Proteases

Morrell

Sadanandom

2019

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Following the evidence that SUMO proteases are important for DELLA activity (Campanaro et al, 2016), the knockout mutant of SUMO protease fug1 showed a higher ratio of overall SUMOylated SLR1. Interestingly, the FUG subclass is phylogenetically close to the SPF subclass, which contains Ulp2-like SUMO proteases (Castro et al, 2018).…”

Section: Discussionmentioning

confidence: 93%

SUMOylation of rice DELLA SLR1 modulates transcriptional responses and improves yield under salt stress

Gonçalves¹,

Fernandes²,

Nunes

et al. 2020

Preprint

View full text Add to dashboard Cite

Author Contributions: 15 NMG participated in the conception of research, executed most experiments, and 16 wrote the article with contributions from all authors. TF did protein interaction 17 experiments. MTGR characterized SUMO-protease rice lines. CCM analysed data 18 and completed the writing. MAAR purified recombinant proteins. PMB analysed RNA 19 seq. MMO supervised experiments and analysed results. IAA conceived original 20 project, supervised experiments, and finalised the article. 31 32 Short title (50 characters): SUMOylation of rice SLR1 increases salt tolerance 33 34 ABSTRACT 35 DELLA proteins modulate GA signalling and are major regulators of plant plasticity to 36 endure stress. DELLAs are mostly regulated at the post-translational level, and their 37 activity relies on the interaction with upstream regulators and transcription factors 38 (TFs). SUMOylation is a post-translational modification (PTM) capable of changing 39protein interaction and found to influence DELLA activity in Arabidopsis. We 40 determined that SUMOylation of the single rice DELLA SLENDER RICE1 (SLR1) 41 occurs in a lysine residue different from the one identified in Arabidopsis 42 REPRESSOR OF GA (RGA), which suggests differential DELLA regulation by 43SUMOylation in monocots and dicots. Remarkably, artificially increasing 44 SUMOylated SLR1 (SUMO1SLR1) levels attenuated the penalty of salt stress on 45 plant yield. Gene expression analysis revealed that the overexpression of 46 SUMOylated SLR1 regulates key dioxygenases that modulate active GA levels, 47namely GA20ox2 and GA2ox3, which could partially explain the sustained 48 productivity upon salt stress imposition. Besides, SLR1 SUMOylation blocked the 49 interaction with the growth regulator YAB4, which may fine-tune GA20ox2 50 expression. Mechanistically, we propose that SLR1 SUMOylation disrupts the 51 interaction with members of several transcription factor families to modulate gene 52 expression. We found that SLR1 SUMOylation represents a novel mechanism 53 modulating DELLA activity, which attenuates the impact of stress on plant 54 performance. 55 56 gibberellin 58 59 One sentence summary: Rice plants show increased yield under salt stress when 60 its gibberellin transcriptional regulator DELLA protein is artificially SUMOylated.

show abstract

“…esd4 mutants are severely dwarfed and early flowering, with a reduced number of flowers and amount of pollen (Reeves, 2002; Murtas et al , 2003; Villajuana-Bonequi et al , 2014). ots1/ots2 mutants share the first two of these characteristics and exhibit reduced seed numbers in all but ideal conditions (Conti et al , 2008; Campanaro et al , 2016). Fertility is also severely affected in spf1 mutants, with less than half of seeds developing normally, and in the spf1/2 double mutant, in which only 15% of seeds complete full normal development (Liu et al , 2017).…”

Section: Resultsmentioning

confidence: 99%

“…For example, ots1ots2 double mutants exhibit a reduction in stamen elongation which, when rescued by crossing in a DELLA mutation, also restores the otherwise reduced seed formation rate back to wild-type levels (Campanaro et al , 2016). Conversely, some spf1/2 double mutant flowers exhibit increased style length, causing a physical pollination barrier.…”

Section: Resultsmentioning

confidence: 99%

“…The GA–abscisic acid equilibrium mediates germination, the endosperm being the key seed layer in the perception of this equilibrium (Nambara et al , 2010). The DELLA proteins, which are degraded in response to GA, are known SUMOylation targets and play a role in flower development (Campanaro et al , 2016), growth repression in abiotic stress situations (Conti et al , 2008), shade avoidance, and in the equilibrium between growth and defence against biotic stresses mediated by GA and jasmonic acid (Pieterse et al , 2014). All this information leads us to hypothesize that the key to uncovering the importance of SUMOylation in crops may lie in the identification of SUMO targets in GA-related proteins.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation