The<i>Arabidopsis</i>B-Box Zinc Finger Family

Khanna, Rajnish; Kronmiller, Brent; Maszle, Don R.; Coupland, George; Holm, Magnus; Mizuno, Takeshi; Wu, Shu-Hsing

doi:10.1105/tpc.109.069088

Cited by 325 publications

(468 citation statements)

References 38 publications

(60 reference statements)

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“…It is also called BBX24 according to the nomenclature for the B-box transcription factors [14], and we use this nomenclature in the rest of the paper. Despite that BBX24 was originally identified from Arabidopsis as a protein conferring salt tolerance in yeast [15], the majority of its characterized functions are involved in light signaling in Arabidopsis [16].…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis STO/BBX24 negatively regulates UV-B signaling by interacting with COP1 and repressing HY5 transcriptional activity

et al. 2012

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UV-B (280-315 nm)is an integral part of solar radiation and can act either as a stress inducer or as a developmental signal. In recent years, increasing attention has been paid to the low-fluence UV-B-induced photomorphogenic response and several key players in this response have been identified, which include UVR8 (a UV-B-specific photoreceptor), COP1 (a WD40-repeat-containing RING finger protein), HY5 (a basic zipper transcription factor), and RUP1/2 (two UVR8-interacting proteins). Here we report that Arabidopsis SALT TOLERANCE (STO/BBX24), a known regulator for light signaling in plants, defines a new signaling component in UV-B-mediated photomorphogenesis. The bbx24 mutant is hypersensitive to UV-B radiation and becomes extremely dwarfed under UV-B treatment. By contrast, BBX24 overexpression transgenic lines respond much more weakly to UV-B than the bbx24 and wild-type plants. BBX24 expression is UV-B-inducible and its accumulation under UV-B requires COP1. Co-immunoprecipitation experiments indicate that BBX24 interacts with COP1

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Section: Introductionmentioning

confidence: 99%

Arabidopsis STO/BBX24 negatively regulates UV-B signaling by interacting with COP1 and repressing HY5 transcriptional activity

et al. 2012

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“…They physically interact with HY5 through the B-box motifs, promote anthocyanin accumulation in concert with HY5, and LZF1/STH3/BBX22 was shown to be ubiquitinated by COP1 (Datta et al, 2007(Datta et al, , 2008Chang et al, 2008). Some other double B-box proteins, DBB1a/ BBX18, DBB1b/BBX19, and STO/BBX24, have been shown to function negatively in light signaling (Indorf et al, 2007;Kumagai et al, 2008;Khanna et al, 2009).…”

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confidence: 99%

“…CO acts as a positive regulator of the floral transition in response to inductive photoperiods, whereas COL3 has been shown to act as a positive regulator of red light signaling (Putterill et al, 1995;Datta et al, 2006). Both CO and COL3 proteins contain two N-terminal B-box motifs and a CCT (for CO, CO-LIKE, TOC) domain in the C terminus (Wenkel et al, 2006;Chang et al, 2008;Khanna et al, 2009). Several other B-box proteins have been implicated in light-regulated development.…”

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confidence: 99%

“…Several other B-box proteins have been implicated in light-regulated development. STH2/BBX21 and LZF1/STH3/BBX22 contain two B-box motifs in the N terminus but lack the CCT domain (Datta et al, 2007(Datta et al, , 2008Chang et al, 2008;Kumagai et al, 2008;Khanna et al, 2009). STH2/ BBX21 and LZF1/STH3/BBX22 are positive regulators of red, far-red, and blue light signaling.…”

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confidence: 99%

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BBX32, an Arabidopsis B-Box Protein, Functions in Light Signaling by Suppressing HY5-Regulated Gene Expression and Interacting with STH2/BBX21

et al. 2011

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A B-box zinc finger protein, B-BOX32 (BBX32), was identified as playing a role in determining hypocotyl length during a largescale functional genomics study in Arabidopsis (Arabidopsis thaliana). Further analysis revealed that seedlings overexpressing BBX32 display elongated hypocotyls in red, far-red, and blue light, along with reduced cotyledon expansion in red light. Through comparative analysis of mutant and overexpression line phenotypes, including global expression profiling and growth curve studies, we demonstrate that BBX32 acts antagonistically to ELONGATED HYPOCOTYL5 (HY5). We further show that BBX32 interacts with SALT TOLERANCE HOMOLOG2/BBX21, another B-box protein previously shown to interact with HY5. Based on these data, we propose that BBX32 functions downstream of multiple photoreceptors as a modulator of light responses. As such, BBX32 potentially has a native role in mediating gene repression to maintain dark adaptation.

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“…BBX proteins family is a subgroup of zinc-finger transcription factors with an important role in growth and development in plants (Khanna et al, 2009) …”

Section: Isolation and Characterisation Of Ppcol-1mentioning

confidence: 99%

Molecular characterisation of flowering genes in Pongamia Pinnata

Kamde¹

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Pongamia pinnata (Pongamia) is a multipurpose tree that has been used traditionally for medicine, green manure, insecticide, fuel and also a source of timber. It is an indigenous plant to the Indian subcontinent and Southeast Asia, and has been successfully introduced to Northern Australia, New Zealand and several other countries with humid tropical lowlands. Recently, Pongamia has attracted interest as one of the alternative feedstock for biodiesel productions due to its ability to produce seeds with high oil content. The fatty acid methyl ester (FAME) composition of Pongamia met the biodiesel specification standards of USA (ASTMD 6751), Germany (DIN 51606) and European Standard Organisation (EN 14214), and is considered as one of the most suitable sources of biodiesel. Despite the positive attributes described above, Pongamia is not a domesticated species and further research is required for the selection of elite cultivars with desirable traits optimal for large scale production of Pongamia oil. In addition, limited information is currently available regarding the flowering mechanism of Pongamia at the molecular level that could provide the platform for the development of oil-rich seeds.The aim of this thesis is to provide insight and increase the understanding of the genetic network that leads to flowering in Pongamia. Based on large phenotypic diversity of Pongamia, this information could subsequently be used to determine and select traits with unique flowering time that will allow a harvesting window, where harvesting process will not clash with flowering. The first objective of this project is to identify, isolate and characterise the candidate genes that regulate flowering time in Pongamia. This was achieved using molecular biology techniques and bioinformatic approaches based on the information available on flowering mechanisms of Arabidopsis and soybean in the literature. The second objective of this project is to determine the expression pattern of isolated genes during flowering onset. This was achieved through transcriptome methodologies such as RT-PCR and qRT-PCR. The third objective of this project is to investigate the genetic variety of Pongamia. This was achieved through Single Nucleotide Polymorphism (SNPs) analysis and its correlation with the late flowering phenotype of Pongamia.PpCOL-1 and PpPHYB are the two genes that were investigated in this thesis. The orthologue genes of PpCOL-1 and PpPHYB are involved in the flowering regulation of the annual plant Arabidopsis.Since Arabidopsis and Pongamia are both long day plants, it is postulated that these genes might play a role in the flowering time control in Pongamia. PpCOL-1 and PpPHYB were successfully isolated, characterised and compared to their respective orthologue genes in Arabidopsis, Soybean and Medicago. It was found that the amino acid residues within the domain regions in these genes iii are highly conserved in Pongamia. Interestingly, there were no significant differences in the expression patterns of PpCOL-1 and PpPHYB in...

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TheArabidopsisB-Box Zinc Finger Family

Cited by 325 publications

References 38 publications

Arabidopsis STO/BBX24 negatively regulates UV-B signaling by interacting with COP1 and repressing HY5 transcriptional activity

Arabidopsis STO/BBX24 negatively regulates UV-B signaling by interacting with COP1 and repressing HY5 transcriptional activity

BBX32, an Arabidopsis B-Box Protein, Functions in Light Signaling by Suppressing HY5-Regulated Gene Expression and Interacting with STH2/BBX21

Molecular characterisation of flowering genes in Pongamia Pinnata

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