The<i>Arabidopsis</i>ATNRT2.7 Nitrate Transporter Controls Nitrate Content in Seeds

Chopin, Franck; Orsel, Mathilde; Dorbe, Marie-France; Chardon, Fabien; Truong, Hoai‐Nam; Miller, Anthony J.; Krapp, Anne; Daniel‐Vedele, Françoise

doi:10.1105/tpc.107.050542

Cited by 209 publications

(197 citation statements)

References 72 publications

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“…Comparison of the predicted protein sequences of HvNRT2.1 and the rice and Arabidopsis NRT2s using the FingerPRINTScan software (www.ebi.ac.uk/Tools/pfa/fingerprintscan/) revealed AE family signature motifs in both OsNRT2.3a/b and AtNRT2.7 (30). Because AE proteins participate in pH regulation (17,31), these motifs may be important for pH sensing in the plant nitrate transporters.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields

Fan

Tang

Tan

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

311

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Fig. 3. Growth, yield, and NUE of OsNRT2.3b, OsNRT2.3a, and H167R mutation overexpressing lines. (A) Phenotypes and transcriptional and translational expression of OsNRT2.3b-and OsNRT2.3a-overexpressing lines and Nipponbare WT. (B) Phenotypes and transcriptional and translational expression of WT and OsNRT2.3b-H167R mutant-overexpressing lines. (C) Average grain yield and NUE of OsNRT2.3b-(O), OsNRT2.3a-(a-O), and H167R-(H167R) overexpressing lines and WT in field plots. RT-PCR with the specific primers (SI Appendix, Table S10) and Western blot analyses with monoclonal antibodies were performed to identify protein expression levels. NUE = grain yield/applied N fertilizer. Values are mean ± SE (n = 3). a and b above bars indicate significant differences (P < 0.05) between the transgenic lines and WT estimated by one-way ANOVA.

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Section: Discussionmentioning

confidence: 99%

Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields

Fan

Tang

Tan

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

311

226

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“…So far, both the low-affinity nitrate transporter NRT2.7 and the chloride channel, CLCa, were identified as responsible for shortdistance nitrate translocation into vacuoles (De Angeli et al, 2006;Chopin et al, 2007). For long-distance transport, recent studies found that NRT1.5 represents a major mechanism for loading nitrate into xylem vessels (Lin et al, 2008) and NRT1.7 for loading nitrate into the phloem (Fan et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

TheArabidopsisNitrate Transporter NRT1.8 Functions in Nitrate Removal from the Xylem Sap and Mediates Cadmium Tolerance

et al. 2010

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Long-distance transport of nitrate requires xylem loading and unloading, a successive process that determines nitrate distribution and subsequent assimilation efficiency. Here, we report the functional characterization of NRT1.8, a member of the nitrate transporter (NRT1) family in Arabidopsis thaliana. NRT1.8 is upregulated by nitrate. Histochemical analysis using promoter-β-glucuronidase fusions, as well as in situ hybridization, showed that NRT1.8 is expressed predominantly in xylem parenchyma cells within the vasculature. Transient expression of the NRT1.8:enhanced green fluorescent protein fusion in onion epidermal cells and Arabidopsis protoplasts indicated that NRT1.8 is plasma membrane localized. Electrophysiological and nitrate uptake analyses using Xenopus laevis oocytes showed that NRT1.8 mediates low-affinity nitrate uptake. Functional disruption of NRT1.8 significantly increased the nitrate concentration in xylem sap. These data together suggest that NRT1.8 functions to remove nitrate from xylem vessels. Interestingly, NRT1.8 was the only nitrate assimilatory pathway gene that was strongly upregulated by cadmium (Cd2+) stress in roots, and the nrt1.8-1 mutant showed a nitrate-dependent Cd2+-sensitive phenotype. Further analyses showed that Cd2+ stress increases the proportion of nitrate allocated to wild-type roots compared with the nrt1.8-1 mutant. These data suggest that NRT1.8-regulated nitrate distribution plays an important role in Cd2+ tolerance.

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“…NRT2 are inducible (NRT2.1, NRT2.2) or constitutively (NRT2.4-NRT2.6) expressed high-affinity nitrate transporters, among which only a few members have been characterized in detail: NRT2.1 and NRT2.2 were shown to be responsible for nitrate uptake from the soil whereas NRT1.7 determines NO 3 − storage in seeds (Orsel et al 2002;Okamoto et al 2003;Tsay et al 2007;Wirth et al 2007;Li et al 2007;Chopin et al 2007). Three tonoplast-localized members of CLC family in Arabidopsis operate as nitrate transporters: CLCa, which functions as a 2NO 3 -/1H + antiporter critical for NO 3 − accumulation within vacuoles (Geelen et al 2000; de Angeli et al 2006) and CLC-b as well as CLC-c, which regulate the level of vacuolar nitrate in plant cells (Harada et al 2004;von der Fecht-Bartenbach et al 2010).…”

Section: Introductionmentioning

confidence: 99%

The genomic organization and transcriptional pattern of genes encoding nitrate transporters 1 (NRT1) in cucumber

2012

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Background and Aims NRT1 proteins are H + -coupling nitrate transporters which belong to the family of peptide transporters (PTRs) and facilitate low and high affinity nitrate transport systems in a model plant Arabidopsis thaliana. In this study, we present the first inventory of the Cucumis sativus NRT1 family together with the transcriptional profile of CsNRT1 genes suggesting the physiological function of the family members in cucumber. Methods Semiquantitative RT-PCR was used to analyze the level and organ-distribution of expression of CsNRT1 genes. The response of those CsNRT1s, whose transcripts were clearly detectable in vegetative tissues to different level of nitrate supply was examined through real-time PCR assays. ResultsThe newly identified cucumber NRT1s were given the designation according to their homology to A. thaliana AtNRT1s. The comparison of the Arabidopsis and cucumber NRT gene families, similarly to the previous comparison of NRT1s in Arabidopsis, poplar and grasses, reveals some striking differences in genes' structure and quantity. Conclusions The putative function of particular CsNRT1 proteins is discussed, considering the results obtained here as well as the already published studies on A. thaliana NRT1 transporters.

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TheArabidopsisATNRT2.7 Nitrate Transporter Controls Nitrate Content in Seeds

Cited by 209 publications

References 72 publications

Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields

Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields

TheArabidopsisNitrate Transporter NRT1.8 Functions in Nitrate Removal from the Xylem Sap and Mediates Cadmium Tolerance

The genomic organization and transcriptional pattern of genes encoding nitrate transporters 1 (NRT1) in cucumber

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