The genomic organization and transcriptional pattern of genes encoding nitrate transporters 1 (NRT1) in cucumber

Migocka, Magdalena; Warzybok, Anna; Kłobus, Grażyna

doi:10.1007/s11104-012-1345-x

Cited by 24 publications

(21 citation statements)

References 45 publications

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“…Cucumber plants were grown essentially as described earlier [46], with slight modification of nitrogen source and availability in nutrition media. The roots, stems or leaves were collected from 2-week-old plants grown under different source of nitrogen and from 4-week-old plants grown upon different nitrate provision.…”

Section: Methodsmentioning

confidence: 99%

Reliable Reference Genes for Normalization of Gene Expression in Cucumber Grown under Different Nitrogen Nutrition

Warzybok

Migocka

2013

PLoS ONE

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In plants, nitrogen is the most important nutritional factor limiting the yield of cultivated crops. Since nitrogen is essential for synthesis of nucleotides, amino acids and proteins, studies on gene expression in plants cultivated under different nitrogen availability require particularly careful selection of suitable reference genes which are not affected by nitrogen limitation. Therefore, the objective of this study was to select the most reliable reference genes for qPCR analysis of target cucumber genes under varying nitrogen source and availability. Among twelve candidate cucumber genes used in this study, five are highly homologous to the commonly used internal controls, whereas seven novel candidates were previously identified through the query of the cucumber genome. The expression of putative reference genes and the target CsNRT1.1 gene was analyzed in roots, stems and leaves of cucumbers grown under nitrogen deprivation, varying nitrate availability or different sources of nitrogen (glutamate, glutamine or NH3). The stability of candidate genes expression significantly varied depending on the tissue type and nitrogen supply. However, in most of the outputs genes encoding CACS, TIP41, F-box protein and EFα proved to be the most suitable for normalization of CsNRT1.1 expression. In addition, our results suggest the inclusion of 3 or 4 references to obtain highly reliable results of target genes expression in all cucumber organs under nitrogen-related stress.

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Section: Methodsmentioning

confidence: 99%

Reliable Reference Genes for Normalization of Gene Expression in Cucumber Grown under Different Nitrogen Nutrition

Warzybok

Migocka

2013

PLoS ONE

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“…Nitrate was the only N source in the nutrient solutions to eliminate confounding factors such as other N compounds acting as a signal (Cerezo et al, 2001;Tsay et al, 1993). Nitrogen supplied at 10 mM is considered replete for this type of experiment (Migocka et al, 2013;Robinson et al, 2011) and the limiting N supply was set 10-fold lower to induce a differential growth response. After harvest 1 at 55 DAS, each pot was flushed with 1,000 mL of tap water and left to drain.…”

Section: Introductionmentioning

confidence: 99%

Post-anthesis nitrate uptake is critical to yield and grain protein content in Sorghum bicolor

Worland

Robinson

Jordan

et al. 2017

Journal of Plant Physiology

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Crops only use 50% of applied nitrogen (N) fertilizer creating N losses and pollution. Plants need to efficiently uptake and utilize N to meet growing global food demands. Here we investigate how the supply and timing of nitrate affects N status and yield in Sorghum bicolor (sorghum). Sorghum was grown in pots with either 10 mM (High) or 1 mM (Low) nitrate supply. Shortly before anthesis the nitrate supply was either maintained, increased 10-fold or eliminated. Leaf sheaths of sorghum grown with High nitrate accumulated nitrate in concentrations >3-times higher than leaves. Removal of nitrate supply pre-anthesis resulted in the rapid reduction of stored nitrate in all organs. Plants receiving a 10-fold increase in nitrate supply pre-anthesis achieved similar grain yield and protein content and 29% larger grains than those maintained on High nitrate, despite receiving 24% less nitrate over the whole growth period. In sorghum, plant available N is important throughout development, particularly anthesis and grain filling, for grain yield and grain protein content. Nitrate accumulation in leaf sheaths presents opportunities for the genetic analysis of mechanisms behind nitrate storage and remobilization in sorghum to improve N use efficiency.

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“…Both NRT1.5 and NRT1.8 are coded by multiple genes in Brachypodium, cucumber, grape, maize, rice and sorghum NRT1.6 and NRT1.7 are coded by single genes in Brachypodium, poplar and rice. No NRT1.6 and NRT1.7 genes were found in Cucumber, grape, maize and sorghum genomes [305] [433] [434].…”

Section: Plant Nitrome (Nitro (Gen)ome)mentioning

confidence: 99%

“…No NRT1.4 homolog could be found in poplar. In cucumber NRT1.4A is specifically expressed only in young and old petioles, while NRT1.4B is expressed in cotyledons and leaves [305] [434]. NRT1.5 is coded by multiple genes in poplar (3), rice (2), maize (2), sorghum (2), Brachypodium (2), grape (2) and cucumber (3) (Both in rice and cucumber NRT1.5A gene is expressed all over the plant while NRT1.5B in rice is expressed in panicle.…”

Section: Evolution Of Nitrogen Metabolism Genesmentioning

confidence: 99%

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Nitrogen Nutrition, Its Regulation and Biotechnological Approaches to Improve Crop Productivity

Reddy¹,

Ulaganathan²

2015

AJPS

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Nitrogen is the most important macronutrient needed for plant growth and development. The availability of nitrogen in the soil fluctuates greatly in both time and space. Crop plants, except leguminous plants, depend on supply of nitrogen as fertilizers. Large quantities of nitrogen fertilizers are applied to crop plants, but only 33% of it is utilized by the plant. Plants have developed efficient mechanisms to sense the varying levels of nitrogen forms and uptake them. They also have well developed mechanisms to assimilate the incoming nitrogen immediately or translocate to different parts of the plant wherever it is needed. Maintenance of nitrogen homeostasis is essential to avoid toxicity. Apart from translocation and assimilation, plants have developed different mechanisms, nitrogen efflux; vacuolar nitrogen storage and downward transport of nitrogen from aerial parts to roots, for maintaining nitrogen homeostasis. In crop plants the "grain yield per unit of available nitrogen in the soil" is referred as the nitrogen use efficiency (NUE) for which remobilization of nitrogen, mediated by various transporters plays a crucial role. All these processes are tightly regulated by proteins and microRNA in response to both external and internal nitrogen levels, carbon status of the plant and hormones. As most crop plants are non-leguminous and depend on soil nitrogen, more production could be achieved if crop plants can be made to utilize the available nitrogen efficiently. The recent explosion of research information and the mechanisms behind nitrogen sensing, signaling, transport and utilization enables biotechnological interventions for better nitrogen nutrition of crop plants. This review discusses such possibilities in the context of recent understanding of nitrogen nutrition and the genomic revolution sweeping the crop science.

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The genomic organization and transcriptional pattern of genes encoding nitrate transporters 1 (NRT1) in cucumber

Cited by 24 publications

References 45 publications

Reliable Reference Genes for Normalization of Gene Expression in Cucumber Grown under Different Nitrogen Nutrition

Reliable Reference Genes for Normalization of Gene Expression in Cucumber Grown under Different Nitrogen Nutrition

Post-anthesis nitrate uptake is critical to yield and grain protein content in Sorghum bicolor

Nitrogen Nutrition, Its Regulation and Biotechnological Approaches to Improve Crop Productivity

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