The hypolipidemic effects of peptides prepared from <scp><i>Cicer arietinum</i></scp> in ovariectomized rats and HepG2 cells

Shi, Wen; Hou, Tao; Liu, Weiwei; Guo, Dayong; He, Hui

doi:10.1002/jsfa.9218

Cited by 16 publications

(14 citation statements)

References 51 publications

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“…In male rats, a 100 mg per kilogram of BW per day dose of a chickpea protein hydrolysate produced by alcalase was the most effective dose in reducing overall cholesterol concentrations in the body of male rats eating high‐fat (HFD) and nonhigh‐fat diets (Shi, Hou, Guo, et al, ). In female rats, a 200 mg per kilogram of BW per day dose of a chickpea protein hydrolysate formed through alcalase was the most effective dose in improving markers associated with hyperlipidemia (Shi, Hou, Liu, et al, ). The chickpea protein hydrolysate formed through alcalase showed to decrease cholesterol in rats by inhibiting enzymes that promote cholesterol synthesis in cells and increasing the fecal excretion of cholesterol (Table ).…”

Section: Biological Activity Of Chickpea Peptidesmentioning

confidence: 99%

“…The chickpea protein hydrolysate formed through alcalase showed to decrease cholesterol in rats by inhibiting enzymes that promote cholesterol synthesis in cells and increasing the fecal excretion of cholesterol (Table ). The peptide VFVRN was identified in the chickpea protein hydrolysate produced through alcalase mentioned, but its hypolipidemic effects have only been tested in cells (Shi, Hou, Guo, et al., ; Shi, Hou, Liu, et al., ). VFVRN was shown to decrease the expression and activity of HMG‐CoA, a key enzyme in cholesterol synthesis (Table ).…”

Section: Biological Activity Of Chickpea Peptidesmentioning

confidence: 99%

“…Alcalase has been widely used in the production of chickpea protein hydrolysates and peptides. Chickpea protein hydrolysates formed by alcalase alone have been shown to have potential antioxidant, ACE inhibitory, and hypolipidemic bioactives (Medina-Godoy et al, 2012;Shi, Hou, Liu, Guo, & He, 2019;Zhang, Jiang, Miao, Mu, & Li, 2012). Alcalase was used to hydrolyze chickpea protein isolate to produce the peptide NRYHE, whose antioxidant activity has been well studied in both chemical and cellular models (Guo, Zhang, Jiang, Miao, & Mu, 2014;Zhang, Li, Miao, & Jiang, 2011).…”

Section: Microbial Proteases (Alcalase and Flavorzyme)mentioning

confidence: 99%

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Enzymatic Production, Bioactivity, and Bitterness of Chickpea (Cicer arietinum) Peptides

Hernandez

Mejía

2019

Comp Rev Food Sci Food Safe

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Chickpeas are inexpensive, protein rich (approximately 20% dry mass) pulses available worldwide whose consumption has been correlated with positive health outcomes. Dietary peptides are important molecules derived from dietary proteins, but a comprehensive analysis of the peptides that can be produced from chickpea proteins is missing in the literature. This review provides information from the past 20 years on the enzymatic production of peptides from chickpea proteins, the reported bioactivities of chickpea protein hydrolysates and peptides, and the potential bitterness of chickpea peptides in food products. Chickpea peptides have been enzymatically produced with pepsin, trypsin, chymotrypsin, alcalase, flavorzyme, and papain either alone or in combination, but the sequences of many of the peptides in chickpea protein hydrolysates remain unknown. In addition, a theoretical hydrolysis of chickpea legumin by stem bromelain and ficin was performed by the authors to highlight the potential use of these enzymes to produce bioactive chickpea peptides. Antioxidant activity, hypocholesterolemic, and angiotensin 1‐converting enzyme inhibition are the most studied bioactivities of chickpea protein hydrolysates and peptides, but anticarcinogenic, antimicrobial, and anti‐inflammatory effects have also been reported for chickpea protein hydrolysates and peptides. Chickpea bioactive peptides are not currently commercialized, but their bitterness could be a major impediment to their incorporation in food products. Use of flavorzyme in the production of chickpea protein hydrolysates has been proposed to decrease their bitterness. Future research should focus on the optimization of chickpea bioactive peptide enzymatic production, studying the bioactivity of chickpea peptides in humans, and systematically analyzing chickpea peptide bitterness.

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Section: Biological Activity Of Chickpea Peptidesmentioning

confidence: 99%

Section: Biological Activity Of Chickpea Peptidesmentioning

confidence: 99%

Section: Microbial Proteases (Alcalase and Flavorzyme)mentioning

confidence: 99%

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Enzymatic Production, Bioactivity, and Bitterness of Chickpea (Cicer arietinum) Peptides

Hernandez

Mejía

2019

Comp Rev Food Sci Food Safe

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“…All assays were carried out in triplicate and the resulted data were expressed as means ± standard deviation (SD, n = 3). ANOVA test using SPSS 19.0 was applied to analyze the experimental data. Significant differences among the parameters means were determined by using Duncan's multiple range test.…”

Section: Discussionmentioning

confidence: 99%

“…In an ovariectomized rats model, chickpea peptides (ChPs) decreased the adipose tissue size, body weight, TC, TG, LDL, cholesterol, liver TC and TG, and serum atherogenic index [19]. The mechanism was that ChPs could inhibit the activities of HMGR and FA synthetase (FAS), down-regulate the expression of SREBP-1c and peroxisome proliferator-activated receptors (PPAR)γ, and up-regulate the expression of estrogen receptor (ER)α, ERβ, and liver X receptor (LXR)α.…”

Section: Introductionmentioning

confidence: 99%

Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

Wang

Pan

et al. 2020

Applied Sciences

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In this work, the hypolipidemic activities of two pentapeptides (VIAPW and IRWWW) from miiuy croaker (Miichthys miiuy) muscle on oleic acid (OA)-induced lipid accumulation in HepG2 cells were investigated. VIAPW and IRWWW could significantly inhibit lipid accumulation induced by OA and decreased intracellular levels of intracellular triglyceride (TG) and total cholesterol (TC) in a dose-effect dependence manner. At the concentration of 100 μm, the TG levels of VIAPW (0.201 ± 0.006 mm) and IRWWW (0.186 ± 0.005 mm) were very (p < 0.01) and extremely (p < 0.001) significantly lower than those (0.247 ± 0.004 mm) of the OA model group; the levels of TC of VIAPW (45.88 ± 0.74 μg/mg protein) and IRWWW (41.02 ± 0.14 μg/mg protein) were very (p < 0.01) and extremely (p < 0.001) significantly lower than that (53.45 ± 0.10μg/mg protein) of the OA model group (p < 0.01). The hypolipidemic mechanisms of VIAPW and IRWWW were to down-regulate the expression levels of genes of SREBP-1c, SREBP-2, FAS, ACC, and HMGR in lipid synthesis and to up-regulate the expression levels of genes of PPARα, ACOX-1, and CPT-1 in lipid oxidation. These results suggested that VIAPW and IRWWW could play their hypolipidemic activities in HepG2 cells through regulation of AMPK pathway and act as hypolipidemic nutrient ingredients applied in public healthy and functional foods.

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Study of active components and mechanisms mediating the hypolipidemic effect of Inonotus obliquus polysaccharides

Ding,

Guo,

et al. 2024

Food Science & Nutrition

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Hyperlipidemia is a multifaceted metabolic disease, which is the major risk factor for atherosclerosis and cardiovascular diseases. Traditional Chinese medicine provides valuable therapeutic strategies in the treatment of hyperlipidemia. Inonotus obliquus has been used in traditional medicine to treat numerous diseases for a long time. To screen and isolate the fractions of I. obliquus polysaccharides (IOP) that can reduce blood lipid in the hyperlipemia animals and cell models, and investigate its mechanisms. The active component IOP‐A2 was isolated, purified, and identified. In vivo, rats were randomly divided into blank control group (NG), the high‐fat treatment group (MG), lovastatin group (PG), and IOP‐A group. Compared with MG, the hyperlipidemic rats treated with IOP‐A2 had decreased body weight and organ indexes, with the level of serum total cholesterol (TC), triglyceride (TG), and low‐density lipoprotein cholesterol (LDL‐C) significantly decreased (p < .05), and level of serum high‐density lipoprotein cholesterol (HDL‐C) significantly increased (p < .05). Hepatocyte steatosis in hepatic lobules was significantly reduced. In vitro, the accumulation of lipid droplets in the model of fatty degeneration of HepG2 cells was significantly alleviated, and cellular TC and TG content was significantly decreased (p < .01). Moreover, the expression of recombinant cytochrome P450 7A1 (CYP7A1) and Liver X Receptor α (LXRα) were up‐regulated (p < .05) both in vivo and in vitro. The results showed that IOP‐A2 may exert its hypolipidemic activity by promoting cholesterol metabolism and regulating the expression of the cholesterol metabolism‐related proteins CYP7A1, LXRα, SR‐B1, and ABCA1.

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The hypolipidemic effects of peptides prepared from Cicer arietinum in ovariectomized rats and HepG2 cells

Abstract: We demonstrated that ChPs could effectively regulate lipid metabolism disorders and restrain obesity caused by estrogen deficiency. Val-Phe-Val-Arg-Asn identified from ChPs could reduce the expression of HMGR to inhibit cholesterol biosynthesis. © 2018 Society of Chemical Industry.

Cited by 16 publications

References 51 publications

Enzymatic Production, Bioactivity, and Bitterness of Chickpea (Cicer arietinum) Peptides

Enzymatic Production, Bioactivity, and Bitterness of Chickpea (Cicer arietinum) Peptides

Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

Study of active components and mechanisms mediating the hypolipidemic effect of Inonotus obliquus polysaccharides

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