Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

Abstract

In this work, the hypolipidemic activities of two pentapeptides (VIAPW and IRWWW) from miiuy croaker (Miichthys miiuy) muscle on oleic acid (OA)-induced lipid accumulation in HepG2 cells were investigated. VIAPW and IRWWW could significantly inhibit lipid accumulation induced by OA and decreased intracellular levels of intracellular triglyceride (TG) and total cholesterol (TC) in a dose-effect dependence manner. At the concentration of 100 μm, the TG levels of VIAPW (0.201 ± 0.006 mm) and IRWWW (0.186 ± 0.005 mm) were very (p < 0.01) and extremely (p < 0.001) significantly lower than those (0.247 ± 0.004 mm) of the OA model group; the levels of TC of VIAPW (45.88 ± 0.74 μg/mg protein) and IRWWW (41.02 ± 0.14 μg/mg protein) were very (p < 0.01) and extremely (p < 0.001) significantly lower than that (53.45 ± 0.10μg/mg protein) of the OA model group (p < 0.01). The hypolipidemic mechanisms of VIAPW and IRWWW were to down-regulate the expression levels of genes of SREBP-1c, SREBP-2, FAS, ACC, and HMGR in lipid synthesis and to up-regulate the expression levels of genes of PPARα, ACOX-1, and CPT-1 in lipid oxidation. These results suggested that VIAPW and IRWWW could play their hypolipidemic activities in HepG2 cells through regulation of AMPK pathway and act as hypolipidemic nutrient ingredients applied in public healthy and functional foods.

Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill (Euphausia superba) Proteins

“…Bioactive peptides are composed of 2 to 20 amino acid residues and released from their parent proteins using different methods of hydrolysis, including enzymatic hydrolysis, chemical hydrolysis, and biological fermentation [ 11 , 12 , 13 ]. Beyond the recognized nutritional value, bioactive peptides exhibited various biological functions, including hypolipidemic [ 14 ], hypotensive [ 15 ], anticoagulant [ 16 ], anticancer [ 17 , 18 ], and antimicrobial activities [ 19 ]. Among the most studied bioactive peptides, antioxidant peptides derived from marine living resources and their processing by-products, such as yellowfin tuna ( Thunnus albacares ) skin [ 20 ], monkfish muscle [ 21 , 22 ], red tilapia ( Oreochromis sp.)…”

Lipid Inhibitory Effect of (−)-loliolide Isolated from Sargassum horneri in 3T3-L1 Adipocytes: Inhibitory Mechanism of Adipose-Specific Proteins

“…Hypolipidemic effects of marine-derived polysaccharides have been reported [ 39 , 40 , 41 , 42 ]. Nevertheless, few anti-obesity studies have been published on marine peptides, polyphenolic compounds, and pigments [ 23 , 43 , 44 , 45 ]. Therefore, to fill this knowledge gap, we investigated the potential inhibitory effect of the phenolic compound (−)-loliolide isolated from S. horneri on lipid accumulation in 3T3-L1 cells.…”

Preparation, Identification, Molecular Docking Study and Protective Function on HUVECs of Novel ACE Inhibitory Peptides from Protein Hydrolysate of Skipjack Tuna Muscle

“…The cell viability of TMAP1 and TMAP2 on HUVECs were assessed using the MTT assay on manufacturer’s instructions [ 65 , 66 ]. In brief, cells were incubated in 96-well plates at density of 0.8 × 10 4 cells per well with 180 μL completed DMEM.…”