The hydroxyl radical scavengers dimethylsulfoxide and dimethylthiourea protect rats against thioacetamide-induced fulminant hepatic failure

Bruck, Rafael; Aeed, Hussein; Shirin, Haim; Matas, Zipora; Zaidel, Liliana; Avni, Yona; Halpern, Zamir

doi:10.1016/s0168-8278(99)80160-3

Cited by 131 publications

(96 citation statements)

References 59 publications

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“…Oxidative stress has been demonstrated in rats with thioacetamide-induced fulminant hepatic failure (Bruck et al 1999;Reddy et al 2004;Sathyasaikumar et al 2007) with blood and brain ammonia levels of ∼1.5 mM and ∼710 µM respectively (Sathyasaikumar et al 2007). In our present study, the 4-week PCA model of chronic hyperammonemia and minimal HE was not accompanied with an induction of oxidative stress.…”

Section: Discussionsupporting

confidence: 50%

“…This increase was seen at 160 min following the injection, but returned to baseline levels after 240 min, suggesting this ammonia-induced ROS increase is transient. Numerous studies have demonstrated that treatment with antioxidants against ammonia-induced oxidative stress is very efficacious (Bruck et al 1999;Guerrini 1994;Widmer et al 2007) implying oxidative stress is treatable and reversible. However it is uncertain whether the beneficial effect of antioxidants is a direct effect on attenuating oxidative stress or solely by ameliorating liver function and subsequently reducing ammonia.…”

Section: Discussionmentioning

confidence: 99%

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Portacaval anastomosis-induced hyperammonemia does not lead to oxidative stress

et al. 2010

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Yang, X. et al., 2010. Portacaval anastomosis-induced hyperammonemia does not lead to oxidative stress. Metabolic Brain Disease, 25(1), ABSTRACTAmmonia is neurotoxic and believed to play a major role in the pathogenesis of hepatic encephalopathy (HE). It has been demonstrated, in vitro and in vivo, that acute and high ammonia treatment induces oxidative stress. Reactive oxygen species (ROS) are highly reactive and can lead to oxidization of proteins resulting in protein damage. The present study was aimed to assess oxidative status of proteins in plasma and brain (frontal cortex) of rats with 4-week portacaval anastomosis (PCA). Markers of oxidative stress, 4-hydroxy-2-nonenal (HNE) and carbonylation were evaluated by immunoblotting in plasma and frontal cortex. Western blot analysis did not demonstrate a significant difference in either HNE-linked or carbonyl derivatives on proteins between PCA and sham-operated control rats in both plasma and frontal cortex. The present study suggests PCA-induced hyperammonemia does not lead to systemic or central oxidative stress.

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Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Portacaval anastomosis-induced hyperammonemia does not lead to oxidative stress

et al. 2010

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“…Reactive oxygen intermediates contributes to the pathogenesis of various hepatic disorders such as paracetamol intoxication, hemochromatosis, toxic hepatitis, and alcoholic liver injury [1][2][3][4] . Increased oxygen radical production leads to lipid peroxidation by induced cytochrome P4502E1 [5,6] .…”

Section: Introductionmentioning

confidence: 99%

“…Nacetylcysteine (NAC) exerts a strong antioxidant activity, and is the treatment of choice in acetaminophen intoxication. NAC provides protection from toxic liver damage by elevating intacellular glutathione concentrations [1,11] . It has also been used in the treatment of CCl 4 poisoning [12] .…”

Section: Introductionmentioning

confidence: 99%

N-acetylcysteine attenuates alcohol-induced oxidative stress in the rat

Özaras

Tahan²,

Aydın³

et al. 2003

WJG

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AIM:There is increasing evidence that alcohol-induced liver damage may be associated with increased oxidative stress. We aimed to investigate free-radical scavenger effect of nacetylcysteine in rats intragastrically fed with ethanol. METHODS:Twenty-four rats divided into three groups were fed with ethanol (6 g/kg/day, Group 1), ethanol and nacetylcysteine (1 g/kg, Group 2), or isocaloric dextrose (control group, Group 3) for 4 weeks. Then animals were sacrificed under ether anesthesia, intracardiac blood and liver tissues were obtained. Measurements were performed both in serum and in homogenized liver tissues. Malondialdehyde (MDA) level was measured by TBARS method. Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels were studied by commercial kits. Kruskal-Wallis test was used for statistical analysis. RESULTS:ALT and AST in Group 1 (154 U/L and 302 U/L, respectively) were higher than those in Group 2 (94 U/L and 155 U/L) and Group 3 (99 U/L and 168 U/L) (P=0.001 for both). Serum and tissue levels of MDA in Group 1 (1.84 nmol/ mL and 96 nmol/100 mg-protein) were higher than Group 2 (0.91 nmol/mL and 64 nmol/100 mg-protein) and Group 3 (0.94 nmol/mL and 49 nmol/100 mg-protein) (P<0.001 for both). On the other hand, serum GSH-Px level in Group 1 (8.21 U/g-Hb) was lower than Group 2 (16 U/g-Hb) and Group 3 (16 U/g-Hb) (P<0.001). Serum and liver tissue levels of SOD in Group 1 (11 U/mL and 26 U/100 mg-protein) were lower than Group 2 (18 U/mL and 60 U/100 mg-protein) and Group 3 (20 U/mL and 60 U/100 mg-protein) (P<0.001 for both). CONCLUSION:This study demonstrated that ethanolinduced liver damage is associated with oxidative stress, and co-administration of n-acetylcysteine attenuates this damage effectively in rat model. Ozaras R, Tahan V, Aydin S, Uzun H, Kaya S, Senturk H. Nacetylcysteine attenuates alcohol-induced oxidative stress in the rat. World J Gastroenterol 2003; 9(1): 125-128

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“…32 Therefore, these data suggest that Lycium barbarum may aggravate the extent of liver injury by increasing hydroxyl radical release in this model. In this study, Lycium barbarum treatment significantly improved intestinal damage in the TAAinduced liver injury model (Figure 2c).…”

Section: Groups Necrosis Inflammationmentioning

confidence: 63%

Effects of Lycium barbarum on bacterial translocation in thioacetamide-induced liver injury in rats

et al. 2015

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Background and study aim: The aim of the present study was to investigate the effects of Lycium barbarum (LB) on bacterial translocation (BT) frequency in thioacetamide (TAA)-induced liver injury in rats. Materials and methods: Group 1 was the control. In group 2 (TAA), rats received TAA daily for 3 days. In group 3 (TAA+LB), Lycium barbarum was administered orally 25 mg/kg for 21 days prior to the first TAA injection. In group 4 (LB), rats received only Lycium barbarum. Results: In our study, Lycium barbarum treatment did not attenuate liver damage. Lycium barbarum treatment decreased ileal E. coli counts and intestinal damage but it did not alter BT frequency. Conclusions: In conclusion, the effects of Lycium barbarum on BT may be related to ongoing severe liver damage in this model.

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The hydroxyl radical scavengers dimethylsulfoxide and dimethylthiourea protect rats against thioacetamide-induced fulminant hepatic failure

Cited by 131 publications

References 59 publications

Portacaval anastomosis-induced hyperammonemia does not lead to oxidative stress

Portacaval anastomosis-induced hyperammonemia does not lead to oxidative stress

N-acetylcysteine attenuates alcohol-induced oxidative stress in the rat

Effects of Lycium barbarum on bacterial translocation in thioacetamide-induced liver injury in rats

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