The hydrophobic surface of PaAMP from pokeweed seeds is essential to its interaction with fungal membrane lipids and the antifungal activity

Peng, Cheng; Dong, Chunxia; Hou, Qiaomin; Xu, Chunyu; Zhao, Jindong

doi:10.1016/j.febslet.2005.03.046

Cited by 7 publications

(6 citation statements)

References 23 publications

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“…For relationship between structure and activity, linearization of cyclic antimicrobial peptides generally alters their activities as well as their ability to interact with cell membranes28–33. Furthermore, Peng et al reported that the positive patch and the hydrophobic surface were both important for the antifungal function of Pa ‐AMP from poke weed ( P. americana ) seeds34. Most of antimicrobial peptides with chitin‐binding capability were classified into knottin type or hevein type.…”

Section: Discussionmentioning

confidence: 99%

The chitin‐binding capability of Cy‐AMP1 from cycad is essential to antifungal activity

Yokoyama

Iida

Kawasaki

et al. 2009

Journal of Peptide Science

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Antimicrobial peptides are important components of the host innate immune responses by exerting broad-spectrum microbicidal activity against pathogenic microbes. Cy-AMP1 found in the cycad (Cycas revoluta) seeds has chitin-binding ability, and the chitin-binding domain was conserved in knottin-type and hevein-type antimicrobial peptides. The recombinant Cy-AMP1 was expressed in Escherichia coli and purified to study the role of chitin-binding domain. The mutants of Cy-AMP1 lost chitin-binding ability completely, and its antifungal activity was markedly decreased in comparison with native Cy-AMP1. However, the antimicrobial activities of the mutant peptides are nearly identical to that of native one. It was suggested that the chitin-binding domain plays an essential role in antifungal, but not antimicrobial, activity of Cy-AMP1.

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Section: Discussionmentioning

confidence: 99%

The chitin‐binding capability of Cy‐AMP1 from cycad is essential to antifungal activity

Yokoyama

Iida

Kawasaki

et al. 2009

Journal of Peptide Science

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“…Another study showed the relationship between the hydrophobic character of a small protein and its antifungal activity, reported by Peng et al . [ 29 ], who produced mutant genes of a specific seed to obtain a change in the protein-surface hydrophobicity, and showed that a hydrophobic surface is essential to increase the antifungal property.…”

Section: Resultsmentioning

confidence: 99%

Effects of a Thiosemicarbazide Camphene Derivative on Trichophyton mentagrophytes

et al. 2009

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Thiosemicarbazides are compounds known for their biological activity, particularly their antimicrobial properties, which include activity against fungi. The difficulty of treating fungal diseases induced us to assess the antifungal properties of some novel thiosemicarbazide compounds. We selected the natural products limonene and camphene as sources for the preparation of these new thiosemicarbazide derivatives. The compound N(4)-[2,2-dimethyl-3-methylnorbornane]-thiosemicarbazide (TIO C) showed an antifungal effect on Trichophyton mentagrophytes, with values of MIC = 55 μmol L-1 and MFC = 110 μmol L-1. Scanning-electron microscopy showed a decrease in mycelium development and morphological alterations of T. mentagrophytes cultured on nail fragments and treated with TIO C. In an attempt to discover its mode of action, we noted that ergosterol is apparently not a target of TIO C activity. An effect of TIO C on T. mentagrophytes cell walls and dividing cross-walls was shown by observed impairment of the fluorescence of tissues stained with calcofluor white, a specific marker for fungal chitin, suggesting that the compound can affect and damage the cell-wall structure or may interfere with its formation, during cell division, growth, and morphogenesis. This approach to the synthesis of new derivatives might provide interesting compounds with greater biological activity in pharmacological research.

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“…There is little information on the mechanism of action of these peptides beyond the fact that the P. americana knottin binds to the sphingolipids of fungal membranes and its antifungal activity is critically dependent on the presence of the hydrophobic patch (Peng et al 2005). As for biotechnological applications, one of the M. jalapa peptides increased resistance to the fungus Alternaria solani when introduced in tomato (Schaefer et al 2005).…”

Section: Knottins and Cyclotidesmentioning

confidence: 98%

New Antimicrobial Agents of Plant Origin

Sampedro

Valdivia

2013

Antimicrobial Compounds

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Plants are constantly under attack by microbial pathogens. As part of their defensive arsenal, they use antimicrobial peptides such as thionins, defensins, lipid transfer proteins, hevein-like peptides, knottins, cyclotides, b-barrelins, and others. In addition, they produce a diversity of antimicrobial metabolites. Those where the evidence for a role in plant defense is stronger include benzoxazinoids, camalexin, and glucosinolates among the alkaloids; flavonoids and stilbenes among the phenylpropanoids; and also terpenoids such as saponins. Our understanding of these plant antimicrobial agents has increased significantly in recent years with new information on their distribution, synthesis, regulation, in vivo function, and mechanism of action. Plant antimicrobial agents have a large potential for biotechnological applications. Engineered plants with increased disease resistance have been achieved using almost every family of antimicrobial peptides. There have been also been successes in using metabolic engineering to increase the production of antimicrobial compounds, as in the case of stilbenes and glucosinolates. Commercial applications using both approaches are likely to appear soon. The use of plant antimicrobials in human medicine is probably further in the future, although there are promising antifungal agents like defensin peptides, and saponins. With more than a quarter million species and a particularly diverse specialized metabolism, the richness of plant antimicrobials has barely been explored. Plant Antimicrobial DefensesWild plants are quite successful at keeping bacterial and fungal pathogens at bay. In addition to physical barriers, they have an immune system capable of detecting and responding to the presence of pathogens. The first layer of defense is based on

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The hydrophobic surface of PaAMP from pokeweed seeds is essential to its interaction with fungal membrane lipids and the antifungal activity

Cited by 7 publications

References 23 publications

The chitin‐binding capability of Cy‐AMP1 from cycad is essential to antifungal activity

The chitin‐binding capability of Cy‐AMP1 from cycad is essential to antifungal activity

Effects of a Thiosemicarbazide Camphene Derivative on Trichophyton mentagrophytes

New Antimicrobial Agents of Plant Origin

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