The humoral immune response of sheep to antigens from larvae of the sheep blowfly (Lucilia cuprina)

Skelly, Patrick J.; Howells, A. J.

doi:10.1016/0020-7519(87)90160-3

Cited by 27 publications

(10 citation statements)

References 23 publications

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“…In the same way, sheep in field conditions sometimes have a much lower infestation of larvae, which are difficult to detect by necropsy. This phenomenon has also been noted in other parasite species causing myasis in livestock (Robertson, 1980;Skelly & Howells, 1987). The data obtained showed the high sensitivity of the ELISA tests by contrast with a low specificity.…”

Section: Discussionsupporting

confidence: 71%

Analysis of larval antigens of Oestrus ovis for the diagnosis of oestrosis by enzyme‐linked immunosorbent assay

Alcaide

Reina

Frontera

et al. 2005

Medical Vet Entomology

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A serodiagnostic test for the diagnosis of infestation by the sheep nasal bot fly, Oestrus ovis (Linné) was examined. The enzyme-linked immunosorbent assay (ELISA) technique was used to analyze and compare the production of immunoglobulin G (IgG) antibodies against excretory-secretory products (ESP) and crude extract (CE) antigens from all the different larval stages of O. ovis in the sera of 276 adult sheep sampled in summer (n = 135) and winter (n = 141). ESP from first stage larvae was the most sensitive, coating antigen in winter and ESP from second stage larvae during summer. The most specific values were obtained by ESP against L1 in winter and by CE against L3 in summer. These results show that the stage of larval development has a significant impact on the humoral immune response over the course of a season. A significant correlation (P < 0.001) was found between the number of O. ovis larvae and the serum antibody levels using all differents antigens, except L3 CE. In Spain, where a long favourable period exists for the evolution and development of the different stage larvae between March and November, the ELISA test using L1 ESP antigen during winter and L2 ESP antigen in summer may be used for ovine oestrosis immunodiagnosis.

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Section: Discussionsupporting

confidence: 71%

Analysis of larval antigens of Oestrus ovis for the diagnosis of oestrosis by enzyme‐linked immunosorbent assay

Alcaide

Reina

Frontera

et al. 2005

Medical Vet Entomology

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“…A very weak naturally acquired resistance to the Lucilia cuprina blowfly strike is developed in sheep after repeated infestations of larvae [32]. A strong humoral response is induced by excretory-secretory products of the larvae especially the salivary glands and midgut extracts [29] and can significantly inhibit larval growth in vitro [28]. However, this inhibitory effect was not observed in in vivo tests, even with purified serine proteases from ESP [33].…”

Section: No Of No Of L1 No Of L1-l2 No Of L2 No Of L3mentioning

confidence: 99%

“…Several trials of vaccination with ESP were carried out against common sheep parasites such as Fasciola hepatica [30,34] and Haemonchus contortus [27] or myiasis producing species such as Lucilia cuprina in sheep [28,29,32,33] or Hypoderma lineatum in cattle [2,9,24,26] resulting in the reduced parasite burdens and/or fecundity or larval growth.…”

Section: Introductionmentioning

confidence: 99%

Immunisation of lambs with excretory secretory products of Oestrus ovis third instar larvae and subsequent experimental challenge

Frugère¹,

Leon

Prévot³

et al. 2000

Vet. Res.

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-Excretory-secretory products (ESP) of myiasis producing agents are involved in nutrition and development of larvae and are often immunogens. This study was carried out in order to define the antigenicity, the immunogenicity of Oestrus ovis ESP and the role of sheep immune response to ESP. Twenty-four six to eight month old female lambs were randomly allocated into two groups. The first one was immunised twice, four weeks apart, with excretory-secretory products of Oestrus ovis third instar larvae (L3ESP) in complete then incomplete Freund adjuvant. The second one served as a control, and received two injections of PBS plus complete and incomplete Freund adjuvant. Fifteen and twenty-eight days after the second immunisation, animals of both groups were experimentally challenged with O. ovis first instar larvae. Twelve days after the second experimental challenge, the twenty-four lambs were necropsied. The total number of O. ovis larvae, their stages of development, weights and sizes were recorded per animal and compared between the two groups. Establishment rates were very similar in both groups: 39% and 35% in control and vaccinated groups respectively but the percentage of developing stages was higher in the control group (13%) than in the vaccinated group (6%). It was concluded that the L3ESP immunisation of sheep did not protect against larval establishment but provided an inhibitory effect on larval growth.Oestrus ovis / excretory-secretory products / sheep / immunisation / experimental challenge Vet. Res. 31 (2000) 527-535 527

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“…The humoral immune responses to infection was first reported back in the early 1980s (36), and several groups have further investigated the nature of both the systemic and local humoral response during infection (28,(37)(38)(39)(40)(41). These humoral responses have been shown to be directed against E/S products of the larvae (42,43) and the salivary antigens (37) following natural infection, while the response has been characterized by the production of IgG (36,41) with subsequent analysis identifying a number of isotypes including IgG1, IgG2, IgA and IgM (44) and IgE (45) with the primary isotype being identified as IgG1 (42).…”

Section: Sheep Blowfly Infectionsmentioning

confidence: 99%

The immunobiology of myiasis infections – whatever happened to vaccination?

Sandeman

Bowles

Colwell³

2014

Parasite Immunology

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The current state of myiasis vaccine technologies are reviewed mainly in the primary research genera of Lucilia and Hypoderma. The importance of myiasis flies as primary causes of morbidity and mortality in agricultural species and man has not diminished despite the existence of good control strategies. However, the development of vaccines against myiasis infections has been relatively quiescent for more than 10 years despite the rapid development of genomic and proteomic analysis and of skills in data interpretation. The value of vaccine research in an era of chemical primacy is analysed. In fact, recent findings of drug resistance and the impact of animal welfare concerns should mean a renewed interest in alternative controls. The reasons that this has not been true to date are explored and new possibilities discussed.

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The humoral immune response of sheep to antigens from larvae of the sheep blowfly (Lucilia cuprina)

Cited by 27 publications

References 23 publications

Analysis of larval antigens of Oestrus ovis for the diagnosis of oestrosis by enzyme‐linked immunosorbent assay

Analysis of larval antigens of Oestrus ovis for the diagnosis of oestrosis by enzyme‐linked immunosorbent assay

Immunisation of lambs with excretory secretory products of Oestrus ovis third instar larvae and subsequent experimental challenge

The immunobiology of myiasis infections – whatever happened to vaccination?

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