The Human Platelet Membrane Proteome Reveals Several New Potential Membrane Proteins

Moebius, Jan; Zahedi, René P.; Lewandrowski, Urs; Berger, C; Walter, Ulrich; Sickmann, Albert

doi:10.1074/mcp.m500209-mcp200

Cited by 150 publications

(141 citation statements)

References 47 publications

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“…Many drug targets are membrane proteins, and thus there is considerable interest in the plasma membrane subproteome. 122 Dedicated studies used gel-based 99,103,123 or gel-free 108 approaches, with the latter yielding ≈650 membrane proteins, identifying even low abundant 7 transmembrane domain receptors, such as the P2Y 1 receptor for ADP (≈150 copies per platelet). These studies clearly extended our knowledge of plasma membrane proteins and receptors.…”

Section: Platelet Subproteomesmentioning

confidence: 99%

What Can Proteomics Tell Us About Platelets?

Burkhart

Gambaryan

Watson

et al. 2014

Circulation Research

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105

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More than 130 years ago, it was recognized that platelets are key mediators of hemostasis. Nowadays, it is established that platelets participate in additional physiological processes and contribute to the genesis and progression of cardiovascular diseases. Recent data indicate that the platelet proteome, defined as the complete set of expressed proteins, comprises >5000 proteins and is highly similar between different healthy individuals. Owing to their anucleate nature, platelets have limited protein synthesis. By implication, in patients experiencing platelet disorders, platelet (dys)function is almost completely attributable to alterations in protein expression and dynamic differences in post-translational modifications. Modern platelet proteomics approaches can reveal (1) quantitative changes in the abundance of thousands of proteins, (2) post-translational modifications, (3) protein–protein interactions, and (4) protein localization, while requiring only small blood donations in the range of a few milliliters. Consequently, platelet proteomics will represent an invaluable tool for characterizing the fundamental processes that affect platelet homeostasis and thus determine the roles of platelets in health and disease. In this article we provide a critical overview on the achievements, the current possibilities, and the future perspectives of platelet proteomics to study patients experiencing cardiovascular, inflammatory, and bleeding disorders.

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Section: Platelet Subproteomesmentioning

confidence: 99%

What Can Proteomics Tell Us About Platelets?

Burkhart

Gambaryan

Watson

et al. 2014

Circulation Research

Self Cite

105

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“…Very recently, the integration of proteomic, genomic, and bioinformatic data of platelets has been applied in order to obtain a better understanding of platelet functions in health and disease [140,155,156]. Several reviews related to different aspects of platelet proteomics have been reported [157][158][159][160][161][162][163][164].…”

Section: Plateletsmentioning

confidence: 99%

Vascular proteomics

Vivanco

Mas

Dardé

et al. 2007

Proteomics Clinical Apps

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The characterization of patients with acute coronary syndromes (ACS) at the molecular and cellular levels provides a novel vision for understanding the pathological and clinical expression of the disease. Recent advances in proteomic technologies permit the evaluation of systematic changes in protein expression in many biological systems and have been extensively applied to cardiovascular diseases (CVD). The cardiovascular system is in permanent intimate contact with blood, making blood-based biomarker discovery a particularly worthwhile approach. Thus, proteomics can potentially yield novel biomarkers reflecting CVD, establish earlier detection strategies, and monitor response to therapy. Here we review the different proteomic strategies used in the study of atherosclerosis and the novel proteins differentially expressed and secreted by atherosclerotic lesions which constitute novel potential biomarkers (HSP-27, Cathepsin D). Special attention is paid to MS-Imaging of atheroma plaque and the generation, for the first time, of 2-D images of lipids, showing the distribution of these molecules in the different areas of the atherosclerotic lesions. In addition new potential biomarkers have been identified in plasma (amyloid A1a, transtherytin), circulating cells (protein profile in monocytes from ACS patients) and individual cells constituents of atheroma plaques (endothelial, VSMC, macrophages) which provide novel insights into vascular pathophysiology.

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“…The 1-DE can be combined with many chromatographic techniques such as RP separation of proteins, and has proved to be very successful in the analysis of complex membrane proteins. Cao et al [68] analysed isolated PM proteins using SDS-PAGE and microcapillary RP LC-MS/MS to identify 428 proteins, including 204 integral membrane or membraneassociated proteins.…”

Section: Modern Strategies For the Expression And Functional Proteomimentioning

confidence: 99%

Membrane and membrane‐associated proteins involved in the aggressive phenotype displayed by highly invasive cancer cells

2008

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Invasion, the penetration of tumour cells into adjacent tissues, is a fundamental characteristic of malignant carcinomas and a first step in the metastatic process. The molecular mechanisms involved in tumour cell invasion are complex, but over the last couple of decades the knowledge base has grown quite considerably and many proteins with important roles in invasion have been identified and characterised. Benign tumours typically are encapsulated, which inhibits their ability to behave in a malignant manner, meaning these tumours do not grow in a location-limited less aggressive manner, do not invade surrounding tissues and do not metastasise. The ability of malignant tumours to invade and metastasise is the major cause of death for cancer patients. A greater insight into the molecular basis of cancer invasion and metastasis will lead to the development of novel therapies and specific panels of biomarkers for use in the treatment and diagnosis/monitoring in many types of metastatic cancer.

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The Human Platelet Membrane Proteome Reveals Several New Potential Membrane Proteins

Cited by 150 publications

References 47 publications

What Can Proteomics Tell Us About Platelets?

What Can Proteomics Tell Us About Platelets?

Vascular proteomics

Membrane and membrane‐associated proteins involved in the aggressive phenotype displayed by highly invasive cancer cells

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