What Can Proteomics Tell Us About Platelets?

Burkhart, Julia M.; Gambaryan, Stepan; Watson, Steve P.; Jurk, Kerstin; Walter, Ulrich; Sickmann, Albert; Heemskerk, Johan W. M.; Zahedi, René P.

doi:10.1161/circresaha.114.301598

Cited by 105 publications

(87 citation statements)

References 196 publications

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“…126,127 Subproteome evaluations provide insight into subcellular distribution of proteins and have also been undertaken successfully for platelets. 128 Proteomics is most effective when combined with other technologies or approaches rather than used as a stand-alone method. Integration of multiple -omics data can reveal differentially regulated networks at transcriptomic and proteomic levels.…”

Section: The Human Proteomementioning

confidence: 99%

Transformative Impact of Proteomics on Cardiovascular Health and Disease

Lindsey¹,

Mayr²,

Gomes³

et al. 2015

Circulation

140

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Abstract-The year 2014 marked the 20th anniversary of the coining of the term proteomics. The purpose of this scientific statement is to summarize advances over this period that have catalyzed our capacity to address the experimental, translational, and clinical implications of proteomics as applied to cardiovascular health and disease and to evaluate the current status of the field. Key successes that have energized the field are delineated; opportunities for proteomics to drive basic science research, facilitate clinical translation, and establish diagnostic and therapeutic healthcare algorithms are discussed; and challenges that remain to be solved before proteomic technologies can be readily translated from scientific discoveries to meaningful advances in cardiovascular care are addressed. Proteomics is the result of disruptive technologies, namely, mass spectrometry and database searching, which drove protein analysis from 1 protein at a time to protein mixture analyses that enable large-scale analysis of proteins and facilitate paradigm shifts in biological concepts that address important clinical questions. Over the past 20 years, the field of proteomics has matured, yet it is still developing rapidly. The scope of this statement will extend beyond the reaches of a typical review article and offer guidance on the use of next-generation proteomics for future scientific discovery in the basic research laboratory and clinical settings.

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Section: The Human Proteomementioning

confidence: 99%

Transformative Impact of Proteomics on Cardiovascular Health and Disease

Lindsey¹,

Mayr²,

Gomes³

et al. 2015

Circulation

140

View full text Add to dashboard Cite

show abstract

“…Recent proteomic studies show just how diverse is this pool [2]. Inherited platelet bleeding disorders affecting a-granule biogenesis and platelet function, therefore, have a wide interest, both clinically and from a biological view [3].…”

Section: Introductionmentioning

confidence: 99%

Should any genetic defect affecting α‐granules in platelets be classified as gray platelet syndrome?

Nurden

2016

American J Hematol

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There is much current interest in the role of the platelet storage pool of α‐granule proteins both in hemostasis and non‐hemostatic events. As well as in the arrest of bleeding, the secreted proteins participate in wound healing, inflammation, and innate immunity while in pathology they may be actors in arterial thrombosis and atherosclerosis as well as cancer and metastasis. For a long time, gray platelet syndrome (GPS) has been regarded as the classic inherited platelet disorder caused by an absence of α‐granules and their contents. While NBEAL2 is the major source of mutations in GPS, other gene variants may give rise to significant α‐granule deficiencies in platelets. These include GATA1, VPS33B, or VIPAS39 in the arthrogryposis, renal dysfunction, and cholestasis (ARC) syndrome and now GFI1B. Nevertheless, many phenotypic differences are associated with mutations in these genes. This critical review was aimed to assess genotype/phenotype variability in disorders of platelet α‐granule biogenesis and to urge caution in grouping all genetic defects of α‐granules as GPS. Am. J. Hematol. 91:714–718, 2016. © 2016 Wiley Periodicals, Inc.

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“…Through genome-wide association and proteomic studies, a number of genes have been recognized to play a role in platelet activity. 4,5 Efforts to identify and study these genes have utilized various animal models, including zebrafish. The zebrafish, Danio rerio, has risen to become a popular choice to analyze gene function during development and model human disease in vivo.…”

mentioning

confidence: 99%

Simple and Rapid Quantification of Thrombocytes in Zebrafish Larvae

Huarng

Shavit

2015

Zebrafish

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Platelets are a critical component of hemostasis, with disorders of number or function resulting in coagulation disturbances. Insights into these processes have primarily been realized through studies using mammalian models or tissues. Increasingly, zebrafish embryos and larvae have been used to study the protein and cellular components of hemostasis and thrombosis, including the thrombocyte, a nucleated platelet analog. However, investigations of thrombocytes have been somewhat limited due to lack of a robust and simple methodology for quantitation, an important component of platelet studies in mammals. Using video capture, we have devised an assay that produces a rapid, reproducible, and precise measurement of thrombocyte number in zebrafish larvae by counting fluorescently tagged cells. Averaging 1000 frames, we were able to subtract background fluorescence, thus limiting assessment to circulating thrombocytes. This method facilitated rapid assessment of relative thrombocyte counts in a population of 372 zebrafish larvae by a single operator in less than 3 days. This technique requires basic microscopy equipment and rudimentary programming, lends itself to high throughput analysis, and will enhance future studies of thrombopoiesis in the zebrafish.

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What Can Proteomics Tell Us About Platelets?

Cited by 105 publications

References 196 publications

Transformative Impact of Proteomics on Cardiovascular Health and Disease

Transformative Impact of Proteomics on Cardiovascular Health and Disease

Should any genetic defect affecting α‐granules in platelets be classified as gray platelet syndrome?

Simple and Rapid Quantification of Thrombocytes in Zebrafish Larvae

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