The Human Homologue (GFI1B) of the Chicken GFI Gene Maps to Chromosome 9q34.13—A Locus Frequently Altered in Hematopoietic Diseases

Rödel, Bernd; Wagner, Torsten; Zörnig, Martin; Niessing, Jürgen; Möröy, Tarik

doi:10.1006/geno.1998.5601

Cited by 29 publications

(25 citation statements)

References 4 publications

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“…At the N terminus they contain a small conserved SNAG (Snail/Gfi1) domain that is also present in other transcriptional repressors (Figure 1). [1][2][3][4] The SNAG domain serves as an interaction domain to recruit proteins that modify histones. 5 At the C terminus both proteins contain six C 2 H 2 -type zinc fingers of which zinc fingers 3-5 are essential for DNA binding.…”

Section: Structure and Expression Regulation Of Gfi1 And Gfi1b Proteinsmentioning

confidence: 99%

Gfi1 and Gfi1b: key regulators of hematopoiesis

2010

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Transcription factor Growth factor independence 1 (Gfi1) is required for multilineage blood cell development, from stem and progenitor cells to differentiated lymphoid and myeloid cells. Gfi1 expression is rapidly induced by cytokines that control both the adaptive and innate immune systems. Gfi1 itself represses the expression of genes implicated in cell survival, proliferation and differentiation. Changes in Gfi1 expression and function have not only been implicated in neutropenia, allergy, autoimmunity and hyperinflammatory responses, but also in lymphoma and more recently in the development of leukemia. In this study, we review how Gfi1 and its paralogue Gfi1b control the development of blood cells, discuss how changes in Gfi1 and Gfi1b function contribute to hematological disease and report on the molecular function of these proteins.

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Section: Structure and Expression Regulation Of Gfi1 And Gfi1b Proteinsmentioning

confidence: 99%

Gfi1 and Gfi1b: key regulators of hematopoiesis

2010

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“…16,19 There is an increasing number of in vitro and in vivo reports on RNAi-mediated silencing of BCR-ABL fusion gene. 9,10,20 These constructs induce target-specific cleavage of BCR-ABL mRNA without affecting the expression of c-BCR or c-ABL mRNA. 21 Moreover, the first report on BCR-ABL siRNA by Wilda et al 22 showed that BCR-ABL silencing was accompanied by strong induction of apoptotic cell death.…”

Section: Discussionmentioning

confidence: 99%

“…The use of several siRNAs to induce additive effects toward target cells has already been described in other systems. 9,10 In experiments focused on cell cycle analysis, regulatory RNA and apoptotic responses induced by RNAi with cotreatment of GFI1B siRNA and BCR-ABL siRNA seemed to be fluctuating. There are several pharmacological mechanisms by which CML cells develop resistance to TKIs or other treatments, such as increased drug-efflux-related surface molecules including MDR1, or aberrant regulation of signal transduction.…”

Section: Discussionmentioning

confidence: 99%

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Additive antileukemia effects by GFI1B- and BCR–ABL-specific siRNA in advanced phase chronic myeloid leukemic cells

Koldehoff¹,

Zakrzewski²,

Beelen³

et al. 2013

Cancer Gene Ther

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Previous studies demonstrated selective inhibition of the BCR-ABL (breakpoint cluster region-Abelson murine leukemia oncogene) tyrosine kinase by RNA interference in leukemic cells. In this study, we evaluated the effect of BCR-ABL small interfering RNA (siRNA) and GFI1B siRNA silencing on chronic myeloid leukemia (CML) cells in myeloid blast crises. The GFI1B gene was mapped to chromosome 9 and is, therefore, located downstream of the BCR-ABL translocation in CML cells. Co-transfection of BCR-ABL siRNA and GFI1B siRNA dramatically decreased cell viability and significantly induced apoptosis and inhibited proliferation in K562 cells (Po0.0001) and primary advanced phase CML cells (Po0.0001) versus controls. Furthermore, combining of BCR-ABL siRNA and GFI1B siRNA significantly modified the expression of several relevant genes including Myc, MDR1, MRP1 and tyrosylphosphoproteins in primary CML cells. Our data suggest that silencing of both BCR-ABL siRNA and GFI1B siRNA is associated with an additive antileukemic effect against K562 cells and primary advanced CML cells, further validating these genes as attractive therapeutic targets.

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“…In 1933, Gilks first identified the GFI1 gene in the integration sites of rat T cell lymphoma, which is located in chromosome 1p22 of the human genome (Gilks et al, 1993, Rödel et al, 1998. In 1998, Rödel (Elmaagacli et al, 2007) found that the GFI1B gene was located in chromosome 9q34.13 of the human genome.…”

Section: Introductionmentioning

confidence: 99%

Associations between polymorphisms of the GFI1B gene and growth traits of indigenous Chinese goats

Cai¹,

Chen²,

Luo³

2014

Genet. Mol. Res.

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ABSTRACT. This study aimed to investigate polymorphisms of the eighth exon in the GFI1B gene among three indigenous Chinese goat breeds (QianBei Ma goats, GuiZhou white goats, and GuiZhou black goats). Furthermore, association analysis was conducted between these polymorphisms and growth traits. Polymerase chain reactionsingle strand conformation polymorphism (PCR-SSCP), direct DNA sequencing, and PCR-restricted fragment length polymorphism (RFLP) were applied to detect polymorphism sites, and a general linear model was used to analyze their association with growth traits. We found two consistent single nucleotide polymorphism (SNP) sites in the eighth exon of the GFI1B gene among the three breeds: 263 bp G→T and 340 bp G→A. The fixed effects model used to analyze growth traits revealed significant differences in body weight, body length, chest depth, and chest breadth between genotypes CD, CC, and DD (P < 0.01). The 340(G/C) polymorphic sites identified here will provide a basis to further study associations between the GFI1B gene and growth Polymorphism of GFI1B gene and growth traits of goat traits, as well as establish a theoretical foundation to develop better feeding and genetic resources of indigenous goats.

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The Human Homologue (GFI1B) of the Chicken GFI Gene Maps to Chromosome 9q34.13—A Locus Frequently Altered in Hematopoietic Diseases

Cited by 29 publications

References 4 publications

Gfi1 and Gfi1b: key regulators of hematopoiesis

Gfi1 and Gfi1b: key regulators of hematopoiesis

Additive antileukemia effects by GFI1B- and BCR–ABL-specific siRNA in advanced phase chronic myeloid leukemic cells

Associations between polymorphisms of the GFI1B gene and growth traits of indigenous Chinese goats

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