Associations between polymorphisms of the GFI1B gene and growth traits of indigenous Chinese goats

Cai, Hongneng; Chen, Z; Luo, Wenjun

doi:10.4238/2014.february.13.5

Cited by 8 publications

(6 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Qianbei Ma goats are resistant to adversity and disease, and retain the desirable traits of meekness, low odor, tender meat, and early sexual maturity ( 35 ). It is a valuable local breed in Guizhou Province, and the average litter size of it is lower than other domestic goat breeds ( 36 , 37 ). To determine whether CTSS is a candidate gene for molecular breeding analysis in the high-reproductive performance Qianbei Ma goat population, we examined the expression of CTSS mRNA in the gonadal axis of single and multilamb ewes.…”

Section: Discussionmentioning

confidence: 99%

Effect of CTSS non-synonymous mutations on litter size in Qianbei Ma goats

Zhang,

Chen,

Ruan

et al. 2023

Front. Vet. Sci.

View full text Add to dashboard Cite

Cathepsin S (CTSS) is a member of the cysteine protease family closely related to reproductive regulation in goats. However, its effect on litter size in goats remains unclear. In this study, the relationship between CTSS gene polymorphisms and litter size was revealed by analyzing the DNA sequence and mRNA expression of CTSS in the gonadal axis of Qianbei Ma goats. In addition, bioinformatics methods were used to evaluate the effect of non-synonymous mutations on CTSS protein structure and function. CTSS was expressed in all parts of the gonadal axis of Qianbei Ma goats, with the highest expression in the uterus in the multi-lamb group and in the fallopian tube in the single-lamb group. The sequencing results showed that four SNPs in CTSS, including g.7413C → T, g.8816A → T, g.9191 T → G and g.10193G → A, were significantly correlated with litter size (p < 0.05). All four analyzed mutation sites were in strong linkage disequilibrium (r2 > 0.33, D′ > 0.70). Additionally, the haplotype Hap1/2 had a significantly higher frequency than the other haplotypes (p < 0.05). g.7413C → T and g.8816A → T were non-synonymous mutations. The g.7413C → T mutation resulted in the substitution of serine 161 of the CTSS protein with phenylalanine (p.S161F), and the g.8816A → T mutation resulted in the substitution of aspartate 219 with tyrosine (p.N219Y). p.S161F was highly conserved across 13 species and that p.N219Y was relatively conserved in cloven-hoofed species. Mutations at two sites changed the local conformation of the CTSS protein, reduced its stability, and affected its function and goat breed evolution. These findings confirm that CTSS affects the lambing traits of goats and provide a theoretical basis for the regulatory mechanism of CTSS in affecting litter size.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of CTSS non-synonymous mutations on litter size in Qianbei Ma goats

Zhang,

Chen,

Ruan

et al. 2023

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…The Qianbei Ma goat is one of the three excellent local goat breeds in Guizhou and displays roughage tolerance, strong disease resistance, good grouping, ease of keeping in captivity, a docile temperament, and strong adaptability [16],It has greater development potential and value. Based on the importance of testes for Qianbei Ma goat reproduction,In this experiment, we analyzed the mRNA expression profiles of the testes of Guizhou Qianbei Ma goats at 3 months of age (before sexual maturity) and 9 months of age (after sexual maturity) by transcriptomics, screened the differentially expressed genes in different periods, and explored the functions of the differentially expressed genes to provide basic data for the enhancement of the reproductive ability of Guizhou Qianbei Ma goats.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the gene expression profile of testicular tissue before and after sexual maturity in Qianbei Ma goats

Chen,

Guo

et al. 2024

BMC Vet Res

View full text Add to dashboard Cite

Background With long-term research on the reproductive ability of Qianbei Ma goat, we found that the puberty of the male goats comes at the age of 3 months and reaches sexual maturity at 4 months,the male goats are identified as physically mature at 9 months and able to mate. Compared with other kinds of breeds of goats, Qianbei Ma goat is featured with more faster growth and earlier sexual maturity.Therefore, in order to explore the laws of growth of Qianbei Ma goat before sexual maturity(3-month-old)and after sexual maturity (9-month-old). The testicular tissue was collected to explore their changes in morphology through HE staining, the serum was collected to detect the hormone content, and the mRNA expression profile of the testis was analyzed by transcriptomics. In this way, the effect of testicular development on the reproduction of Qianbei ma goats was further analyzed. Results The results showed that the area and diameter of spermatogenic tubules were larger at 9 months than 3 months, and the number of spermatocytes, interstitial cells, spermatogonia and secondary spermatocytes in the lumen of the tubules showed a similar trend. The appearance of spermatozoa at age 3 months indicated that puberty had begun in Qianbei Ma goats. The Elasa test for testosterone, luteinizing hormone, follicle stimulating hormone and anti-Müllerian hormone showed that the levels of these hormones in the serum at age 9 months were all highly significantly different than those at age 3 months (P < 0.01). There were 490 differentially expressed genes (DEGs) between the (|log2(fold change)| > 1 and p value < 0.05) 3-month-old and 9-month-old groups, of which 233 genes were upregulated and 257 genes were downregulated (3 months of age was used as the control group and 9 months of age was used as the experimental group). According to the GO and KEGG enrichment analyses of DEGs, PRSS58, ECM1, WFDC8 and LHCGR are involved in testicular development and androgen secretion, which contribute to the sexual maturation of Qianbei Ma goats. Conclusions Potential biomarker genes and relevant pathways involved in the regulation of testicular development and spermatogenesis in Qianbei Ma goats were identified, providing a theoretical basis and data support for later studies on the influence of testicular development and spermatogenesis before and after sexual maturity in Qianbei Ma goats.

show abstract

“…Shaanbei white cashmere (SBWC) goats were bred from Liaoning white cashmere goat and Ziwuling black goat ( 19 ), which has high cashmere value and meat value ( 20 ). Guizhou black (GB) goats are an excellent local breed with good meat quality and coarse feeding tolerance ( 21 ). Nubian (NB) goats have good value for meat and milk and have higher meat content than other dual-purpose goats ( 22 ).…”

Section: Introductionmentioning

confidence: 99%

Detection distribution of CNVs of SNX29 in three goat breeds and their associations with growth traits

Wang,

Song,

et al. 2023

Front. Vet. Sci.

View full text Add to dashboard Cite

As a member of the SNX family, the goat sorting nexin 29 (SNX29) is initially identified as a myogenesis gene. Therefore, this study aimed to examine the polymorphism in the SNX29 gene and its association with growth traits. In this study, we used an online platform to predict the structures of the SNX29 protein and used quantitative real-time PCR to detect potential copy number variation (CNV) in Shaanbei white cashmere (SBWC) goats (n = 541), Guizhou black (GB) goats (n = 48), and Nubian (NB) goats (n = 39). The results showed that goat SNX29 protein belonged to non-secretory protein. Then, five CNVs were detected, and their association with growth traits was analyzed. In SBWC goats, CNV1, CNV3, CNV4, and CNV5 were associated with chest width and body length (P < 0.05). Among them, the CNV1 individuals with gain and loss genotypes were superior to those individuals with a median genotype, but CNV4 and CNV5 of individuals with the median genotype were superior to those with the loss and gain genotypes. In addition, individuals with the gain genotype had superior growth traits in CNV3. In brief, this study suggests that the CNV of SNX29 can be used as a molecular marker in goat breeding.

show abstract

Associations between polymorphisms of the GFI1B gene and growth traits of indigenous Chinese goats

Cited by 8 publications

References 14 publications

Effect of CTSS non-synonymous mutations on litter size in Qianbei Ma goats

Effect of CTSS non-synonymous mutations on litter size in Qianbei Ma goats

Comparison of the gene expression profile of testicular tissue before and after sexual maturity in Qianbei Ma goats

Detection distribution of CNVs of SNX29 in three goat breeds and their associations with growth traits

Contact Info

Product

Resources

About