The Human Aldose Reductase AKR1B1 Qualifies as the Primary Prostaglandin F Synthase in the Endometrium

Bresson, Eva; Boucher-Kovalik, Sofia; Chapdelaine, Pierre; Madore, Éric; Harvey, Nathalie; Laberge, Philippe Y.; Lebœuf, Mathieu; Fortier, Michel A.

doi:10.1210/jc.2010-1589

Cited by 54 publications

(65 citation statements)

References 42 publications

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“…The aldose reductase gene (AKR1B1) encodes the enzyme aldolase reductase responsible for metabolizing progesterone, and is involved in the production of prostaglandin F2α by the endometrium in cattle and humans (Madore et al 2003, Bresson et al 2011. This gene was upregulated in biopsies derived from blastocysts which failed to establish a pregnancy after transfer (El-Sayed et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Spatial differences in gene expression in the bovine oviduct

et al. 2016

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The aim of this study was to compare the transcriptome of the oviductal isthmus of pregnant heifers with that of cyclic heifers as well as to investigate spatial differences between the transcriptome of the isthmus and ampulla of the oviduct in pregnant heifers. After synchronizing crossbred beef heifers, those in standing oestrus (=Day 0) were randomly assigned to cyclic (non-bred, n = 6) or pregnant (artificially inseminated, n = 11) groups. They were slaughtered on Day 3 and both oviducts from each animal were isolated and cut in half to separate ampulla and isthmus. Each portion was flushed to confirm the presence of an oocyte/embryo and was then opened longitudinally and scraped to obtain epithelial cells which were snap-frozen. Oocytes and embryos were located in the isthmus of the oviduct ipsilateral to the corpus luteum. Microarray analysis of oviductal cells revealed that proximity to the corpus luteum did not affect the transcriptome of the isthmus, irrespective of pregnancy status. However, 2287 genes were differentially expressed (P < 0.01) between the ampulla and isthmus of the oviduct ipsilateral to the corpus luteum in pregnant animals. Gene ontology revealed that the main biological processes overrepresented in the isthmus were synthesis of nitrogen, lipids, nucleotides, steroids and cholesterol as well as vesicle-mediated transport, cell cycle, apoptosis, endocytosis and exocytosis, whereas cell motion, motility and migration, DNA repair, calcium ion homeostasis, carbohydrate biosynthesis, and regulation of cilium movement and beat frequency were overrepresented in the ampulla. In conclusion, large differences in gene expression were observed between the isthmus and ampulla of pregnant animals at Day 3 after oestrus.Reproduction (2016) 152 37-46

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Section: Discussionmentioning

confidence: 99%

Spatial differences in gene expression in the bovine oviduct

et al. 2016

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“…It has been suggested that an AKR1C subclass is a PGF synthase responsible for PGF 2a production in the porcine endometrium [9]; however, the expression pattern of this enzyme does not seem to correlate with the levels of uterine endometrial and luminal PGF 2a during the estrous cycle and early pregnancy. Interestingly, recent studies have shown that AKR1B1 is the major PGF synthase responsible for PGF 2a synthesis from PGH 2 in the bovine and human uterine endometrium [24][25][26]. It has been reported that AKR1B1 is expressed in the porcine uterine endometrium, proposing that AKR1B1 may be involved in glucose metabolism [27].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Prostaglandin Metabolic Enzyme Expression During Pregnancy and the Characterization of AKR1B1 as a Prostaglandin F Synthase at the Maternal-Conceptus Interface in Pigs1

Seo

Choi

Shim

et al. 2014

Biology of Reproduction

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Prostaglandins (PGs) are important lipid mediators regulating various reproductive processes in many species. In pigs, the expression pattern of PGE2 and PGF2α metabolic enzymes and the regulatory mechanism controlling PGE2 and PGF2α levels in the uterus during pregnancy are not completely understood. This study determined endometrial expression of the genes (PLA2G4A, PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, CBR1, and HPGD) involved in PGE2 and PGF2α metabolism during the estrous cycle and pregnancy and measured levels of PGE2 and PGF2α in uterine endometrial tissues and uterine flushings at the time of conceptus implantation in pigs. Except PTGES3, expression of the genes studied changed in a pregnancy-stage-specific manner, and localization of PTGES, AKR1B1, CBR1, and HPGD mRNAs were cell-type specific in the uterine endometrium. Levels of both PGE2 and PGF2α in uterine endometrial tissues and uterine lumen were higher on Day 12 of pregnancy than those of the estrous cycle and affected by different morphology of spherical and filamentous conceptuses. Furthermore, we determined that endometrial expression of AKR1B1, known to encode a PGF2α synthase in other species, was increased by estrogen and interleukin-1beta and that AKR1B1 exhibited PGF2α synthase activity in the porcine uterine endometrium. These results in pigs indicate that the PGE2 and PGF2α metabolic enzymes are expressed stage specifically in the endometrium during pregnancy and regulate the abundance of PGE2 and PGF2α in the uterus at the time of implantation and that AKR1B1 may act as a major PGF synthase in the endometrium during early pregnancy.

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“…1bed). The status of FP receptor immunoreactivity tended to be correlated with Ki-67 LI (p ¼ 0.0587), but no statistically significant correlations were detected between FP receptor status and any of the clinicopathological parameters (Supplementary Table 2 Previous studies showed that PGF synthases expression levels were altered depending on the menstrual cycle in the endometrium, suggesting that the function or activation of the FP receptor was determined by the expression of these enzymes (Bresson et al, 2011). Therefore, we then immunolocalized currently identified PGF synthases, AKR1C3 and AKR1B1, in the breast cancer tissues detailed above to explore the significance of combined expression of FP receptor and PGF synthase expression.…”

Section: Fp Receptor Expression Had a Trend Towards Association With mentioning

confidence: 98%

“…The antigeneantibody complex was then visualized with 3, 3'-diaminobenzidine (DAB) solution and counterstained with hematoxylin. Normal Endometrium was used as the positive control of immunostaining of PTGFR and AKR1B1 (Dorniak et al, 2011;Bresson et al, 2011), adrenal gland as that of AKR1C3 (Nakamura et al, 2009) and skin epidermis as that of Slug (Mistry et al, 2014).…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…AKR1C3 is known to be abundantly expressed in breast cancer tissues, and high levels are often associated with adverse clinical outcome (Vihko et al, 2005;Oduwole et al, 2004). The other known PGF synthase, AKR1B1 is only recently identified (Bresson et al, 2011;Chapdelaine et al, 2006), and its expression and function have not been previously studied in breast cancers.…”

Section: Introductionmentioning

confidence: 99%

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11β-Prostaglandin F2α, a bioactive metabolite catalyzed by AKR1C3, stimulates prostaglandin F receptor and induces slug expression in breast cancer

Yoda

Kikuchi

Miki

et al. 2015

Molecular and Cellular Endocrinology

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The Human Aldose Reductase AKR1B1 Qualifies as the Primary Prostaglandin F Synthase in the Endometrium

Abstract: The human aldose reductase AKR1B1 currently associated with diabetes complications is also a highly functional PGF synthase responsible for PGF2α production in the human endometrium and a potential target for treatment of menstrual disorders.

Cited by 54 publications

References 42 publications

Spatial differences in gene expression in the bovine oviduct

Spatial differences in gene expression in the bovine oviduct

Comprehensive Analysis of Prostaglandin Metabolic Enzyme Expression During Pregnancy and the Characterization of AKR1B1 as a Prostaglandin F Synthase at the Maternal-Conceptus Interface in Pigs1

11β-Prostaglandin F2α, a bioactive metabolite catalyzed by AKR1C3, stimulates prostaglandin F receptor and induces slug expression in breast cancer

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