Spatial differences in gene expression in the bovine oviduct

Maillo, V.; Frutos, C. de; Ó’Gaora, Peadar; Forde, Niamh; Burns, Gregory W.; Spencer, Thomas E.; Gutiérrez‐Adán, Alfonso; Lonergan, Patrick; Rizos, Dimitrios

doi:10.1530/rep-16-0074

Cited by 48 publications

(43 citation statements)

References 65 publications

(67 reference statements)

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“…Interestingly, gene expression studies of the oviduct in which the early embryo develops show little change due to the presence of an embryo following transfer in the absence of SP (Maillo et al 2015). In fact, further studies reveal that gene expression in the oviduct isthmus following AI is unchanged by the presence of an embryo when compared with oviductal cells at estrus (Maillo et al 2016). The authors conclude from these studies that gene expression in the oviduct, and subsequent production of embryokines, is dependent only on estrous cycle stage.…”

Section: Current Reproductive Strategies In Domestic Species and The mentioning

confidence: 88%

A role for seminal plasma in modulating pregnancy outcomes in domestic species

Bromfield¹

2016

Reproduction

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Seminal plasma is a complex fluid produced by the accessory glands of the male reproductive tract. Seminal plasma acts primarily as a transport medium for sperm on its arduous journey through the male and then female reproductive tract following ejaculation. This spermatozoan expedition will hopefully result in the meeting of and resultant fertilization of an oocyte, perpetuating the genetic lineage of both sexes. Whereas seminal plasma has historically been perceived as only a transport medium providing a nutrient-rich fluid environment for sperm during this exchange of genetic material, new insights into a complex communication pathway between males and females has been unraveled in the past 30 years. This new research suggests seminal plasma as a method to promote early pregnancy success by modulating cellular and molecular adaptions of the maternal environment required to facilitate healthy, successful pregnancy outcomes. Whereas much work on this exciting new communication process has focused on mice and translation to human reproduction, here we review the current evidence in domestic species where artificial insemination in the absence of seminal plasma is routine. Improving artificial insemination in domestic species to optimize offspring health and productivity could have far-reaching impacts on agriculturally relevant species such as cattle, sheep, pigs and horses.Reproduction (2016) 152 R223-R232

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Section: Current Reproductive Strategies In Domestic Species and The mentioning

confidence: 88%

A role for seminal plasma in modulating pregnancy outcomes in domestic species

Bromfield¹

2016

Reproduction

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“…After flushing, each section of the oviduct was opened longitudinally and gently scraped with a blade to recover the epithelial cells. The same procedure has been previously performed by us (Maillo et al, ) in which the epithelial nature of the recovered cells was confirmed by ‘flow cytometry analysis’ using antibodies anti‐bovine‐pancadherin (C1821), anti‐bovine‐pancytokeratin (C2931) and anti‐bovine‐vimentin (V2258). The cells obtained after centrifugation (18.8 g for 1 min at 4°C) were snap‐frozen and stored at −80°C until gene expression analysis.…”

Section: Methodsmentioning

confidence: 91%

“…Sample recovery on Day 2.5 was performed in order to be certain that (a) the embryo would be found within the oviduct and (b) that the embryo location enabled the collection of the oviductal portions surrounding the embryo section (i.e., proximal and distal sections). However, as described, this early embryo recovery entailed finding the embryos around the ampullary–isthmic junction, which may mask the embryo effect due to the spatial differences in gene expression between the two sections of the oviduct (Maillo et al, ). In line with this, the fact that three out of the four embryos recovered at Day 2.5 were at the 2‐cell stage—that is before major embryo genome activation, which in cattle occurs around the 8‐cell stage (Graf et al, )—could have influenced the oviduct response, which in vitro has been demonstrated to be embryo stage‐specific (Hamdi et al, ).…”

Section: Discussionmentioning

confidence: 99%

An approach to study the local embryo effect on gene expression in the bovine oviduct epithelium in vivo

Rodríguez‐Alonso

Hamdi²,

Sánchez

et al. 2019

Reprod Domestic Animals

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This study aimed to examine the local embryo effect on the transcriptomic response of the epithelial cells of the oviduct in vivo. Fifteen heifers were synchronized and artificially inseminated to a standing heat. All heifers were slaughtered on Day 2.5 after oestrus. The oviducts from 13 animals were isolated, trimmed free of tissue and divided between ampulla/isthmus. The ipsilateral isthmus was divided into smaller sections (2 cm). Each section was sequentially flushed until the embryo was located (4/13) and then opened and scraped longitudinally to obtain the epithelial cells. Cells were snap‐frozen in LN2 for gene expression analysis. All recovered embryos were found at the beginning of the isthmus. The 2 cm sections selected for the transcriptomic analysis were as follows: embryo section (in which the embryo was found); proximal section (through which the embryo had passed); distal section (on the uterine side of the embryo); and contralateral section (section from the contralateral isthmus). The expression pattern of eight genes (STK32A, KERA, QRFPR, MCTP1, PRELP, VAT1L, SOCS3 and CCL20) differentially expressed between the isthmus of pregnant (multiple embryo model) and cyclic heifers were assessed by RT‐qPCR. One‐way ANOVA and t test was used for statistical analysis. Comparisons between ipsilateral and contralateral oviduct or along the ipsilateral oviduct resulted in no differences for all genes. Despite the failure to detect a site‐specific response of a single embryo on the abundance of distinct transcripts in the bovine oviduct in vivo on Day 2.5, the current methodology with proposed modifications would be useful for future studies to examine the local embryo effect.

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“…The oviduct is a dynamic organ comprising different anatomical and functional regions that may show differences in mRNA expression levels and in the synthesis and secretion of various proteins (Maillo et al, 2016). Thus, the present study focuses on the expression of MMP2, MMP9, TIMP1 and TIMP2 in the llama oviductal segments and the active and inactive MMP forms present in the OF.…”

Section: Discussionmentioning

confidence: 99%

SegmentSpecific Expression of MMP/TIMP in the Oviduct of Llama (Lama glama) and Gelatinolytic Activity in the Oviductal Fluid

et al. 2017

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SUMMARY:Oviductal molecules have the potential to improve the reproductive biotechnologies. In camelids, knowledge and assessment of the oviductal environment are necessary to successfully develop species-specific reproductive technologies, especially because of the camelids reproductive particularities. Among the oviductal factors, the matrix metalloproteinases/tissue inhibitor of matrix metalloproteinases system (MMPs/TIMPs) should be investigated more thoroughly due to their participation in reproductive processes. Consequently, the current study assayed gene and protein expression of MMPs throughout the llama oviduct. MMPs zymogen and active forms in the oviductal fluid were also characterized. MMP2 and MMP9 transcripts were detected in ampulla, isthmus, uterotubal junction and papilla, being MMP2 and MMP9 2.15 and 1.10 folds higher in papilla than in ampulla, respectively. In addition, differences in immunolocalization of MMP2 and MMP9 between the epithelial mucosa layers of the oviductal segments were observed. The presence of MMPs in the epithelium suggests their secretion into the oviductal lumen. Coincidently, bands of 62 and 94 kDa, corresponding to MMP2 and MMP9 were detected by zymography in the oviductal fluid. Treatment with an exogenous activator (APMA) suggests that they are present as proMMPs. TIMP2 and TIMP1, the specific inhibitors of MMP2 and MMP9, respectively, were expressed in each oviductal segment, indicating a well-regulated control of MMP proteolytic activity in the oviduct. These findings prove that the llama oviduct produces and secretes MMPs into the oviductal lumen, suggesting that these enzymes may have an unknown role in the preparation of the oviductal environment for gametes, fertilization and early embryo development in camelids.

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Spatial differences in gene expression in the bovine oviduct

Cited by 48 publications

References 65 publications

A role for seminal plasma in modulating pregnancy outcomes in domestic species

A role for seminal plasma in modulating pregnancy outcomes in domestic species

An approach to study the local embryo effect on gene expression in the bovine oviduct epithelium in vivo

SegmentSpecific Expression of MMP/TIMP in the Oviduct of Llama (Lama glama) and Gelatinolytic Activity in the Oviductal Fluid

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Spatial differences in gene expression in the bovine oviduct

Cited by 48 publications

References 65 publications

A role for seminal plasma in modulating pregnancy outcomes in domestic species

A role for seminal plasma in modulating pregnancy outcomes in domestic species

An approach to study the local embryo effect on gene expression in the bovine oviduct epithelium in vivo

Segment­Specific Expression of MMP/TIMP in the Oviduct of Llama (Lama glama) and Gelatinolytic Activity in the Oviductal Fluid

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SegmentSpecific Expression of MMP/TIMP in the Oviduct of Llama (Lama glama) and Gelatinolytic Activity in the Oviductal Fluid