The Horse Chestnut Lines Harboring the rol Genes

Zdravković-Korać, Snežana; Ćalić﻿﻿﻿, Dušica; Druart, Philippe; Radojević, Ljiljana

doi:10.1023/b:biop.0000041051.81210.9b

Cited by 19 publications

(14 citation statements)

References 23 publications

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“…The presence of the rol genes in all transgenic hairy root‐derived cultures was confirmed by PCR amplification using gene‐specific primers for rolA and rolB as described by Zdravković‐Korać et al . (2004) and for rolC , as described by Di Guardo et al . (2003) (Table ).…”

Section: Methodsmentioning

confidence: 99%

“…Transformation of H. tomentosum and H. tetrapterum was achieved with wild-type agropine strains of A. rhizogenes A4 and ATCC 15834 (American Type Cultures Collection, Manassas, USA), and the transgenic character of hairy roots was demonstrated according to Komarovsk a et al (2009). [21] The presence of the rol genes in all transgenic hairy rootderived cultures was confirmed by PCR amplification using gene-specific primers for rolA and rolB as described by Zdravkovi c-Kora c et al (2004) [26] and for rolC, as described by Di Guardo et al (2003) [20] (Table 1). The hairy roots were cultivated in the darkness in the MS/B5 medium.…”

Section: Hairy Roots Cultures and Transgenic Plantsmentioning

confidence: 99%

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Chemometric evaluation of hypericin and related phytochemicals in 17 in vitro cultured Hypericum species, hairy root cultures and hairy root-derived transgenic plants

Nigutová

Kusari

Sezgin

et al. 2017

Journal of Pharmacy and Pharmacology

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Section: Methodsmentioning

confidence: 99%

Section: Hairy Roots Cultures and Transgenic Plantsmentioning

confidence: 99%

Chemometric evaluation of hypericin and related phytochemicals in 17 in vitro cultured Hypericum species, hairy root cultures and hairy root-derived transgenic plants

Nigutová

Kusari

Sezgin

et al. 2017

Journal of Pharmacy and Pharmacology

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“…Before using the hairy root cultures in this experiment, the presence of the rol genes (rolA ? rolB) was repeatedly determined by PCR amplification using specific primers as described by Zdravkovic-Korac et al (2004); rolC, as described by Di Guardo et al (2003), and rolD, by Komarovská et al (2009). Roots grown under photoperiod conditions showed the lowest biomass increases among all species tested so the hairy roots were grown only in continuous darkness.…”

Section: Root and Hairy Roots Culturesmentioning

confidence: 99%

Xanthones from roots, hairy roots and cell suspension cultures of selected Hypericum species and their antifungal activity against Candida albicans

et al. 2015

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Highest xanthone contents were found in Hypericum pulchrum and H. annulatum untransformed roots. The best anti- Candida activity was obtained for hairy roots extracts of H. tetrapterum clone 2 ATCC 15834. Extracts of root cultures, hairy roots and cell suspensions of selected Hypericum spp. were screened for the presence of xanthones and tested for their antifungal activity against Candida albicans strain ATCC 10231. At least one of the following xanthones, 5-methoxy-2-deprenylrheediaxanthone; 1,3,6,7-tetrahydroxyxanthone; 1,3,5,6-tetrahydroxyxanthone; paxanthone; kielcorin or mangiferin was identified in methanolic extracts of the untransformed root cultures. The highest total xanthone content, with five xanthones, was found in untransformed H. pulchrum and H. annulatum root cultures. Hairy roots and the controls of H. tetrapterum contained 1,7-dihydroxyxanthone, while hairy root cultures and the corresponding controls of H. tomentosum contained toxyloxanthone B, 1,3,6,7- and 1,3,5,6-tetrahydroxyxanthone. Two xanthones, cadensin G and paxanthone, were identified in cell suspension cultures of H. perforatum. Their content increased about two-fold following elicitation with salicylic acid. The anti-Candida activity of the obtained extracts ranged from MIC 64 to >256 µg ml(-1). Among the extracts of Hypericum untransformed roots, the best antifungal activity was obtained for extracts of H. annulatum grown under CD conditions. Extracts of hairy roots clones A4 and 7 ATCC15834 of H. tomentosum and clone 2 ATCC15834 of H. tetrapterum displayed inhibition of 90% of Candida growth with 256 μg ml(-1). Extracts from chitosan-elicitated cells did not show antifungal activity.

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“…However, differentiated tissues display both genetic and biochemical stability (Flores & Curtis, 1992). For this reason, genetically transformed roots are particularly interesting due to their capacity for long-term growth (Flores et al, 1999;Momčilović et al, 2001;Zdravković-Korać et al, 2003), and therefore widely used as a source of many useful compounds (Flores & Curtis, 1992;Oksman-Caldentey & Hiltunen, 1996;Flores et al, 1999).…”

Section: Resultsmentioning

confidence: 99%

Determination of escin content in androgenic embryos and hairy root culture ofAesculus hippocastanum

Ćalić¹,

Zdravković-Korać²,

Šavikin-Fodulović³

et al. 2010

Pharmaceutical Biology

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The Horse Chestnut Lines Harboring the rol Genes

Cited by 19 publications

References 23 publications

Chemometric evaluation of hypericin and related phytochemicals in 17 in vitro cultured Hypericum species, hairy root cultures and hairy root-derived transgenic plants

Chemometric evaluation of hypericin and related phytochemicals in 17 in vitro cultured Hypericum species, hairy root cultures and hairy root-derived transgenic plants

Xanthones from roots, hairy roots and cell suspension cultures of selected Hypericum species and their antifungal activity against Candida albicans

Determination of escin content in androgenic embryos and hairy root culture ofAesculus hippocastanum

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