The Histopathologic Effects of Balloon Dacryoplasty on the Rabbit Nasolacrimal Duct

“…In a study by Goldstein, Katowitz and Syed [37], the effectiveness of balloon dacryoplasty procedure in humans supports this procedure in treating adults and children with nasolacrimal duct obstruction.…”

Section: Figure 6b Recanalized Lumen Of the Nasolacrimal Ductmentioning

confidence: 97%

Optimizing clinical outcomes for endoscopic lacrimal duct recanalization in patients with complete PANDO

Javate

2022

Int Ophthalmol

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The study aims to describe modi cations and re nements in the technique and technology of Transcanalicular Endoscopic Lacrimal Duct Recanalization (TELDR) based on anatomical foundations to optimize its clinical outcomes in patients with complete Primary Acquired Nasolacrimal Duct Obstruction (PANDO). MethodsThe medical records of 115 patients who underwent Transcanalicular Endoscopic Lacrimal Duct Recanalization procedures from January 2018 to July 2020 were reviewed retrospectively. Of the 115 patients, only those 35 patients with complete PANDO characterized by longstanding epiphora of 5-7 years duration, dense, diffuse brous tissue obstruction involving the sac, sac duct junction and the entire length of the nasolacrimal duct were included in the study. Parameters for success were analyzed based on patency on irrigation, functional endoscopic dye test, and improvement of epiphora. ResultsForty-ve cases from 35 patients with complete PANDO were included in the study. The mean length of time from the date of operation to silicone stent removal was 8.1 weeks while the mean length of followup starting from the removal of silicone stent to last follow-up was 61.0 weeks. There were 95.6% anatomic patency on canalicular irrigation with saline and 95.6% functional patency based on functional endoscopic dye test. There was signi cant improvement of epiphora (p-value of < 0.0001) postoperatively. ConclusionThe results of modi ed TELDR improved clinical outcomes and could be a de nitive treatment in patients with complete PANDO with longstanding, dense, diffuse, brous tissue obstruction. Patients who experience reobstruction, may undergo a repeat of the recanalization approach.

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“…Any stenosis or obstruction in the lacrimal duct system can induce symptoms of epiphora, chronic ocular surface inflammation, and pyorrhea, which may increase the risk of endophthalmitis after intraocular surgery (Lopez et al, 1993;Prabhasawat and Tseng, 1998;Kam et al, 2014). Various surgical treatments, including traditional or laser-assisted dacryocystorhinostomy, silastic tube intubation, balloon dacryoplasty, and laser lacryocystoplasty, can be applied to alleviate obstruction in lacrimal duct systems (Goldstein et al, 2006;Avgitidou et al, 2015;Takahashi et al, 2015). However, the effectiveness of these treatments depends on the integrity of lacrimal duct mucosa (Takahashi et al, 2013;Baran et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

潜在候选细胞构建组织工程的泪道上皮: 兔的组织学和细胞学研究

Xie

Cui

2015

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Injury and deficiency of the lacrimal duct epithelium (LDE) can lead to a variety of lacrimal diseases. The purpose of this study was to characterize potential candidate cells for constructing a tissue-engineered LDE. Methods: Different areas of the conjunctiva and lacrimal duct tissue were removed from male adult New Zealand white rabbits for histological evaluation. Hematoxylin and eosin staining and immunohistochemical staining of cytokeratin AE1+AE3, cytokeratin 4, Ki-67, and MUC5AC were observed by light microscopy. The surface morphologies of different epithelial tissues and cellular structures were examined using field-emission scanning electron microscopy and transmission electron microscopy. Epithelial cells were isolated from tissues and identified by specific markers. In vitro, proliferative ability and Western blot analyses of the proliferating cell nuclear antigen (PCNA) of different epithelial cells cultured in identical environments were investigated and compared. Results: Histologically, the epithelial specific markers, cytokeratin AE1+AE3 and cytokeratin 4, were expressed in the conjunctiva epithelium and the LDE. Notably, highly proliferative cells stained with Ki-67 were concentrated under the epithelium in a dome structure of the posterior palpebral conjunctiva. Differentiated goblet cells were also found to a lesser extent in this region. Primary palpebral and fornical conjunctival epithelial cells (PFCECs), bulbar conjunctival epithelial cells (BCECs), and lacrimal duct epithelial cells (LDECs) were successfully separated from tissues. In vitro, rabbit PFCECs and LDECs grew faster and expressed more PCNA than BCECs. Conclusions: PFCECs are anatomically similar to LDECs. They also have similar morphological characteristics, immune phenotypes, and proliferation features. PFCECs are therefore potential candidate cells to replace LDECs in tissue engineering to treat lacrimal duct diseases.

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The Histopathologic Effects of Balloon Dacryoplasty on the Rabbit Nasolacrimal Duct

Cited by 13 publications

References 12 publications

Dacriocistoplastia con balón catéter en niños con obstrucción nasolagrimal congénita en los que ha fracasado el sondaje

Dacriocistoplastia con balón catéter en niños con obstrucción nasolagrimal congénita en los que ha fracasado el sondaje

Optimizing clinical outcomes for endoscopic lacrimal duct recanalization in patients with complete PANDO

潜在候选细胞构建组织工程的泪道上皮: 兔的组织学和细胞学研究

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