The histone chaperone HIR maintains chromatin states to control nitrogen assimilation and fungal virulence

Jenull, Sabrina; Mair, Theresia; Tscherner, Michael; Penninger, Philipp; Zwolanek, Florian; Silao, Fitz Gerald S.; Vicente, Kontxi Martinez de San; Riedelberger, Michael; Bandari, Naga Chandra; Shivarathri, Raju; Petryshyn, Andriy; Chauhan, Neeraj; Zacchi, Lucía F.; Landmann, Salomé; Ljungdahl, Per O.; Kuchler, Karl

doi:10.1016/j.celrep.2021.109406

Cited by 12 publications

(7 citation statements)

References 158 publications

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“…Whereas in the absence of Hir1, earlier it had been reported that the levels of histone H3 are increased at the promoter of hyphal‐specific genes such as HWP1 and UME6 in the planktonic mode of growth (Jenull et al, 2017) implying a contrasting effect of CAF‐1 and HIR on biofilm circuit regulation. However, the promoters of nitrogen metabolism become more accessible upon Hir1 deletion (Jenull et al, 2021) hinting at a pathway‐specific function for CAF‐1 and HIR chaperone complexes. But unlike mammals, CAF‐1 and HIR subunits are not essential for viability in C. albicans .…”

Section: Discussionmentioning

confidence: 99%

Negative regulation of biofilm development by the CUG‐Ser1 clade‐specific histone H3 variant is dependent on the canonical histone chaperone CAF‐1 complex in Candida albicans

Singha

Aggarwal

Sanyal

2023

Molecular Microbiology

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The CUG‐Ser1 clade‐specific histone H3 variant (H3VCTG) has been reported to be a negative regulator of planktonic to biofilm growth transition in Candida albicans. The preferential binding of H3VCTG at the biofilm gene promoters makes chromatin repressive for the biofilm mode of growth. The two evolutionarily conserved chaperone complexes involved in incorporating histone H3 are CAF‐1 and HIRA. In this study, we sought to identify the chaperone complex(es) involved in loading H3VCTG. We demonstrate that C. albicans cells lacking either Cac1 or Cac2 subunit of the CAF‐1 chaperone complex, exhibit a hyper‐filamentation phenotype on solid surfaces and form more robust biofilms than wild‐type cells, thereby mimicking the phenotype of the H3VCTG null mutant. None of the subunits of the HIRA chaperone complex shows any significant difference in biofilm growth as compared to the wild type. The occupancy of H3VCTG is found to be significantly reduced at the promoters of biofilm genes in the absence of CAF‐1 subunits. Hence, we provide evidence that CAF‐1, a chaperone known to load canonical histone H3 in mammalian cells, is involved in chaperoning of variant histone H3VCTG at the biofilm gene promoters in C. albicans. Our findings also illustrate the acquisition of an unconventional role of the CAF‐1 chaperone complex in morphogenesis in C. albicans.

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Section: Discussionmentioning

confidence: 99%

Negative regulation of biofilm development by the CUG‐Ser1 clade‐specific histone H3 variant is dependent on the canonical histone chaperone CAF‐1 complex in Candida albicans

Singha

Aggarwal

Sanyal

2023

Molecular Microbiology

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“…The ability to properly chaperone histones is paramount to an organism’s ability to respond to changing environments and to preserve genomic integrity, which is crucial for opportunistic fungal pathogens. Recently, the HIR complex subunit Hir1 was shown to coordinate the transcriptional response to sensing nutritional changes, namely, protein as a major nitrogen source through a role in controlling chromatin accessibility in C. albicans ( 38 ). Similarly, Asf1 influences transcriptional responses to stimuli, including nutrient availability ( 39 ) and DNA damage ( 29 ) in S. cerevisiae .…”

Section: Discussionmentioning

confidence: 99%

A J Domain Protein Functions as a Histone Chaperone to Maintain Genome Integrity and the Response to DNA Damage in a Human Fungal Pathogen

Horianopoulos

Lee

Schmitt

et al. 2021

mBio

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“…Read counts obtained from the gene expression analysis were used as the input data in DESeq method to carry out differential expression analysis under different conditions (Anders & Huber, 2010). Genes are considered to be differentially expressed if they have log 2 (fold change), that is log 2 (100 Jm −2 /0 Jm −2 ) values >0.58 (upregulated, fold change >1.5, or > 50% increase in transcript level) or < − 0.58 (down‐regulated, fold change <0.67, or >50% decrease in transcript level) (Jenull et al, 2021; Koeppen et al, 2021; Ravazzolo et al, 2020; Tomasch et al, 2011). Accession numbers for DRPs and house‐keeping genes used in this study were shown in Table S1 and S2.…”

Section: Methodsmentioning

confidence: 99%

Responses of dinoflagellate cells to ultraviolet‐C irradiation

Kwok

Lam

et al. 2022

Environmental Microbiology

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Dinoflagellates are important aquatic microbes and major harmful algal bloom (HAB) agents that form invasive species through ship ballast transfer. UV‐C installations are recommended for ballast treatments and HAB controls, but there is a lack of knowledge in dinoflagellate responses to UV‐C. We report here dose‐dependent cell cycle delay and viability loss of dinoflagellate cells irradiated with UV‐C, with significant proliferative reduction at 800 Jm−2 doses or higher, but immediate LD50 was in the range of 2400–3200 Jm−2. At higher dosages, some dinoflagellate cells surprisingly survived after days of recovery incubation, and continued viability loss, with samples exhibiting DNA fragmentations per proliferative resumption. Sequential cell cycle postponements, suggesting DNA damages were repaired over one cell cycle, were revealed with flow cytometric analysis and transcriptomic analysis. Over a sustained level of other DNA damage repair pathways, transcript elevation was observed only for several components of base pair repair and mismatch repair. Cumulatively, our findings demonstrated special DNA damage responses in dinoflagellate cells, which we discussed in relation to their unique chromo‐genomic characters, as well as indicating resilience of dinoflagellate cells to UV‐C.

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The histone chaperone HIR maintains chromatin states to control nitrogen assimilation and fungal virulence

Cited by 12 publications

References 158 publications

Negative regulation of biofilm development by the CUG‐Ser1 clade‐specific histone H3 variant is dependent on the canonical histone chaperone CAF‐1 complex in Candida albicans

Negative regulation of biofilm development by the CUG‐Ser1 clade‐specific histone H3 variant is dependent on the canonical histone chaperone CAF‐1 complex in Candida albicans

A J Domain Protein Functions as a Histone Chaperone to Maintain Genome Integrity and the Response to DNA Damage in a Human Fungal Pathogen

Responses of dinoflagellate cells to ultraviolet‐C irradiation

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