The histochemistry of mucosaccharides in some organs of germfree rats

Abstract

In order to study the histochemical nature of mucosaccharides in germfree animals, the organs in natural contact with bacteria (stomach, small and large intestine) and those naturally remote from bacteria (tracheal and ear cartilage and aorta) were studied by means of light microscopic methods for mucosaccharides in germfree and conventional rats. In the stomach (surface and foveolar cells) of germfree rats the histochemical reactions for acid and neutral mucosaccharides were apparently less intense than in that of conventional rats, whereas in the small and large intestine (goblet cells) of germfree rats the reactions were significantly more intense than in those of conventional rats. In the cartilage (intercellular matrix, lacunar border and chondrocyte cytoplasm) and aorta (interelastic spaces) of germfree animals the reactions were less intense than in those of conventional animals. In addition, some differences in the histochemical nature of mucosaccharides between the organs of germfree and conventional rats were noted, as revealed by the effects of chemical modifications and digestions with enzymes upon the histochemical reactions studied.

Gnotobiotics and the Microbiome

“…The GF rat appears to be unable to decrease the reabsorption of bile acids in the lower gut, a function of normal microbial flora. Work indicates that differences in the histochemical nature of mucosaccharides are dependent on whether they are located in areas of normal bacterial flora in CV rats or in areas relatively free of intestinal flora (Yamada and Ukai, 1976).…”