The histochemistry of galactose residues of complex carbohydrates as studied by peroxidase-labeled ricinus communis agglutinin

To investigate the histochemical nature of the secretory epithelium lining the goat bulbourethral gland, glycoconjugates contained in these secretory epithelial cells have been studied by means of light- and electron-microscopic histochemistry. The methods employed involved a series of conventional staining and peroxidase-labeled lectin diaminobenzidine procedures together with combined selective methods such as digestion with enzymes. According to the results obtained in the present work, the secretory cells of the goat bulbourethral gland can be grouped into two types: cells with glycoconjugate-rich granules and those with less amounts of glycoconjugates.The gland is at least dual in the nature of its secretory activities.

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“…or Umax flavus agglutinin (LFA)]-diaminobenzidine (DAB) [Kiernan. 1975: Yamada and Shimizu. 1977: Brabecetal.. 1980: Miller etal., 1982: Pearse.…”

Section: Light Microscopymentioning

confidence: 99%

Histochemistry of Glycoconjugates in the Secretory Epithelium of the Goat Bulbourethral Gland

Tsukise

Yamada

1987

Cells Tissues Organs

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“…Digestion with almond glycopeptidase reduced apparently the intensities of both reactions not only in the histological structures of human placenta mentioned ( fig.3c) but in those of human umbilical cord ( fig.3d). In view of the known selectivities of the staining procedures employed here [10,11,15] and the substrate specificity of almond glycopeptidase [1][2][3], the above results show the presence of glycoproteins with asparagine-linked oligosaccharides in the 2 types of tissues.…”

Section: Histochemical Localization Of Oligosaccharidesmentioning

confidence: 63%

“…Deparaffinized sections were prepared routinely from the fixed tissue specimens and subjected to either PO-RCA-DAB [10] or PAS [11] staining procedure. Prior to PAS staining, all the tissue sections were digested with c~-amylase [12].…”

Section: Procedures Of Histochemical Experimentationsmentioning

confidence: 99%

Asparagine‐linked oligosaccharides in human placenta and umbilical cord as demonstrated by almond glycopeptidase

1982

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“…The lectins used were: concanavalin A (Con-A), wheat germ agglutinin (WGA), Griffonia simplicifolia agglutinin-I and -II (GSA-I and -II), Dolichos biflorus agglutinin (DBA), soy bean agglutinin (SBA), Bauhinia purpurea agglutinin (BPA), peanut agglutinin (PNA), Ulex europeaeus agglutinin-I (UEA-I), Limax flavus agglutinin (LFA) (all from E.Y. Labs./Medac and Sigma; for lectin specificity see Yamada & Shimizu 1977, Pearse 1985, Alroy et al 1988, Spicer & Schulte 1992, Danguy et al 1998. The reactions were visualized by peroxidase (PO) conjugated streptavidin, using the 3, 3'diaminobenzidine (DAB)-based BioGenex Super Sensitive Universal Immunostaining Kit (BioGenex Laboratories).…”

Section: Speciesmentioning

confidence: 99%

Possible antimicrobial defense by free sugars on the epidermal surface of aquatic vertebrates

Meyer¹,

Seegers²,

Schnapper³

et al. 2007

Aquat. Biol.

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Biochemical, lectin histochemical, and densitometrical methods demonstrated contents and spectra of free sugars in the skin of aquatic vertebrates (fishes: cod, marine trout, freshwater trout, perch; amphibians: Koa-Tao-Island caecilian, smooth newt, edible frog, South-African clawed toad; mammals: northern fur seal, common seal, otter, Capybara). Total free sugar contents, particularly sialic acids, were relatively higher in freshwater species (perch, Capybara) than in marine species. In amphibians, the caecilian and the newt showed higher amounts of free sugars than the anurans; strong reactions indicated a specific involvement of α-L-fucose in mucus functions. Lectin histochemistry demonstrated positive reactions in the stratum superficiale, the outer stratum spinosum, and specific cells of the fish epidermis. The integumental free sugar spectrum of the amphibians was narrow, with generally lower reaction intensities, except for mucus glands. In the mammals, the epidermal cells exhibited rather weak lectin histochemical staining, except for the northern fur seal and the Capybara, where the upper vital epidermis and corneal cells reacted strongly. The mammalian apocrine skin glands, particularly, exhibited positive reaction staining in their secretory endpiece. The free sugar spectrum was similar in all species: α-D-mannose, β-D-N-acetylglucosamine, α-D-N-acetylgalactosamine, α-D-galactose, β-D-galactose, α-L-fucose, sialic acids (mainly in fish epidermis and skin glands). High concentrations of free sugars on the skin surface possibly impede attacks of commensal skin micro-inhabitants (bacteria, fungi) against the integrity of the epidermis. This features a basic or general biological mechanism that operates before the specific immune system is activated.

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The histochemistry of galactose residues of complex carbohydrates as studied by peroxidase-labeled ricinus communis agglutinin

Cited by 53 publications

References 26 publications

Histochemistry of Glycoconjugates in the Secretory Epithelium of the Goat Bulbourethral Gland

Histochemistry of Glycoconjugates in the Secretory Epithelium of the Goat Bulbourethral Gland

Asparagine‐linked oligosaccharides in human placenta and umbilical cord as demonstrated by almond glycopeptidase

Possible antimicrobial defense by free sugars on the epidermal surface of aquatic vertebrates

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